Patents by Inventor Douglas E. Williams
Douglas E. Williams has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240158398Abstract: Described herein are compounds that inhibit ALK2 and its mutants, pharmaceutical compositions including such compounds, and methods of using such compounds and compositions.Type: ApplicationFiled: April 20, 2023Publication date: May 16, 2024Inventors: Natasja Brooijmans, Jason D. Brubaker, Paul E. Fleming, Brian Lewis Hodous, Joseph L. Kim, Brett D. Williams, Douglas Wilson, Kevin J. Wilson, Mark Cronin
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Publication number: 20240010705Abstract: Disclosed herein are extracellular vesicles comprising an immunomodulating component. Also provided are methods for producing the extracellular vesicles and methods for using the extracellular vesicles for treating cancer, GvHD, and autoimmune diseases.Type: ApplicationFiled: October 26, 2022Publication date: January 11, 2024Applicant: LONZA SALES AGInventors: Nuruddeen D. LEWIS, Yu ZHOU, Sriram SATHYANARAYANAN, John KULMAN, Douglas E. WILLIAMS, Leonid A. GAYDUKOV, Ke XU, Shelly MARTIN
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Publication number: 20240009322Abstract: The present invention relates to methods of preparing a therapeutic exosome using a protein newly-identified to be enriched on the surface of exosomes. Specifically, the present invention provides methods of using the proteins for affinity purification of exosomes. It also provides methods of localizing a therapeutic peptide on exosomes, and targeting exosomes to a specific organ, tissue or cell by using the proteins. The methods involve generation of surface-engineered exosomes that include one or more of the exosome proteins at higher density, or a variant or a fragment of the exosome protein.Type: ApplicationFiled: October 26, 2022Publication date: January 11, 2024Applicant: LONZA SALES AGInventors: Kevin P. DOOLEY, Rane A. HARRISON, Russell E. MCCONNELL, Ke XU, Damian J. HOUDE, Nikki ROSS, Sonya HAUPT, John D. KULMAN, Douglas E. WILLIAMS
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Publication number: 20240000944Abstract: The present invention relates to methods of preparing a therapeutic exosome using proteins newly identified to be enriched in the lumen of exosomes. Specifically, the present invention provides methods of localizing a therapeutic peptide or protein in exosomes. The methods involve generation of lumen-engineered exosomes that include one or more of the exosome proteins at higher concentrations, a modification or a fragment of the exosome protein, or a fusion protein of the exosome protein and a therapeutic or a cargo protein.Type: ApplicationFiled: November 21, 2022Publication date: January 4, 2024Applicant: LONZA SALES AGInventors: Russell E. MCCONNELL, Kevin P. DOOLEY, Rane A. HARRISON, Ke XU, Damian J. HOUDE, Sonya HAUPT, John D. KULMAN, Douglas E. WILLIAMS, Madeleine YOUNISS
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Publication number: 20240002785Abstract: Described herein are methods for the production of extracellular vesicles comprising culturing a population of producer cells in single-cell suspension, wherein the cells are cultured in chemically-defined culture medium, wherein the culture medium lacks animal-derived serum and animal-derived components; and obtaining from the cell culture an extracellular vesicle preparation comprising extracellular vesicles. In certain embodiments, the methods comprise perfusion culturing methods, including single-cell perfusion culturing methods and batch-refeed culturing methods. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.Type: ApplicationFiled: February 21, 2023Publication date: January 4, 2024Applicant: LONZA SALES AGInventors: Agata A. VILLIGER, Andrew F. GRUBE, Tik Yan CHAN, Scott D. ESTES, Kathryn E. GOLDEN, Konstantin KONSTANTINOV, Douglas E. WILLIAMS
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Publication number: 20230365971Abstract: Disclosed herein are compositions and methods comprising extracellular vesicles comprising nucleic acid that target genes, leading to macrophage polarization of tumor associated macrophages. In certain embodiments, disclosed herein are methods and compositions for increasing macrophage polarization for the treatment of cancer.Type: ApplicationFiled: November 4, 2022Publication date: November 16, 2023Applicant: CODIAK BIOSCIENCES, INC.Inventors: Sriram SATHYANARAYANAN, Douglas E. WILLIAMS, Dalia BURZYN, Adam Thomas BOUTIN, Sushrut KAMERKAR
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Publication number: 20230301917Abstract: Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.Type: ApplicationFiled: October 11, 2022Publication date: September 28, 2023Applicant: Codiak BioSciences, Inc.Inventors: Aaron R. NOYES, Michael P. MERCALDI, Kathryn E. GOLDEN, Raymond W. BOURDEAU, Michael F. DOHERTY, Konstantin KONSTANTINOV, Douglas E. WILLIAMS
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Patent number: 11679164Abstract: The present invention relates to methods of preparing a therapeutic exosome using a protein newly-identified to be enriched on the surface of exosomes. Specifically, the present invention provides methods of using the proteins for affinity purification of exosomes. It also provides methods of localizing a therapeutic peptide on exosomes, and targeting exosomes to a specific organ, tissue or cell by using the proteins. The methods involve generation of surface-engineered exosomes that include one or more of the exosome proteins at higher density, or a variant or a fragment of the exosome protein.Type: GrantFiled: December 20, 2019Date of Patent: June 20, 2023Assignee: CODIAK BIOSCIENCES, INC.Inventors: Kevin P. Dooley, Rane A. Harrison, Russell E. McConnell, Ke Xu, Damian J. Houde, Nikki Ross, Sonya Haupt, John D. Kulman, Douglas E. Williams
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Patent number: 11512315Abstract: Disclosed herein are compositions and methods comprising extracellular vesicles comprising nucleic acid that target genes, leading to macrophage polarization of tumor associated macrophages. In certain embodiments, disclosed herein are methods and compositions for increasing macrophage polarization for the treatment of cancer.Type: GrantFiled: February 12, 2019Date of Patent: November 29, 2022Assignee: CODIAK BIOSCIENCES, INC.Inventors: Sriram Sathyanarayanan, Douglas E. Williams, Dalia Burzyn, Adam Thomas Boutin, Sushrut Kamerkar
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Publication number: 20220326158Abstract: Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of intrinsic fluorescence of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.Type: ApplicationFiled: March 28, 2022Publication date: October 13, 2022Applicant: Codiak BioSciences, Inc.Inventors: Damian J. HOUDE, Young Jun CHOI, John D. KULMAN, Douglas E. WILLIAMS
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Publication number: 20220249373Abstract: The present disclosure relates to heterologous exosomal vesicle proteins (HEVPs), engineered exosomes comprising HEVPs, and methods of preparing and using these compositions, including therapeutic applications.Type: ApplicationFiled: February 4, 2020Publication date: August 11, 2022Applicant: Codiak BioSciences, Inc.Inventors: Kevin P. DOOLEY, Douglas E. WILLIAMS, James E. THORNTON
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Publication number: 20220144942Abstract: The present disclosure provides a therapeutic method of treating cancer metastasis by inducing clearance of EVs using a binding agent specific to an EV protein. The method utilizes one or more binding agents specific to EV proteins, where the EV proteins are selected from prostaglandin F2 receptor negative regulator (PTGFRN); basigin (BSG); immunoglobulin superfamily member 2 (IGSF2); immunoglobulin superfamily member 3 (IGSF3); immunoglobulin superfamily member 8 (IGSF8); integrin beta-1 (ITGB1); integrin alpha-4 (ITGA4); 4F2 cell-surface antigen heavy chain (SLC3A2); a class of ATP transporter proteins (ATP1A1, ATP1A2, ATP1A3, ATP1A4, ATP1B3, ATP2B1, ATP2B2, ATP2B3, ATP2B4); CD13 (aminopeptidase N); MME (neprilysin), ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase family member 1); and NRP1 (neuropilin-1). Further provided herein includes a pharmaceutical composition for the treatment of cancer metastasis.Type: ApplicationFiled: February 4, 2020Publication date: May 12, 2022Applicant: CODIAK BIOSCIENCES, INC.Inventors: Kevin P. DOOLEY, Douglas E. WILLIAMS
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Publication number: 20220017907Abstract: The present disclosure relates to therapeutic exosomes enriched in proteins that are present in the luminal surface of exosomes. The present disclosure provides methods of manufacturing exosomes enriched in proteins that are present in the luminal surface of exosomes, method of associating a therapeutic peptide or protein to the luminal surface of exosomes, and method of use, e.g., methods of therapeutic or diagnostic use. The methods of manufacture involve generating of luminal-surface-engineered exosomes that include one or more of the EV, e.g., exosome proteins at concentrations higher that those observed in wild type exosomes, a modification or a fragment of the EV, e.g., exosome protein, or a fusion protein of the EV, e.g., exosome protein, and a payload, e.g., biologically active molecule such as a therapeutic protein.Type: ApplicationFiled: May 22, 2019Publication date: January 20, 2022Applicant: Codiak BioSciences, Inc.Inventors: Kevin P. DOOLEY, Rane A. HARRISON, Ke XU, Damian J. HOUDE, Sonya HAUPT, John D. KULMAN, Douglas E. WILLIAMS, Russell E. MCCONNELL, Madeleine YOUNISS
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Publication number: 20210395721Abstract: Compositions and methods for reducing nucleotide oxidation during electroporation, specifically the use of free radical scavengers to reduce electroporation-induced oxidation, are described. Compositions and methods for enhancing transfection efficiency are also described.Type: ApplicationFiled: October 23, 2019Publication date: December 23, 2021Applicants: Codiak BioSciences, Inc., MaxCyte, Inc.Inventors: Raymond W. BOURDEAU, Delai CHEN, Rane HARRISON, Kathryn E. GOLDEN, Douglas E. WILLIAMS, Sergey DZEKUNOV, Madhusudan PESHWA
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Publication number: 20210322327Abstract: Provided herein are compositions comprising EV, e.g., exosome, encapsulated STING agonists and methods of producing the compositions described. Also provided herein are methods of modulating an immune response via administration of a therapeutic amount of EV, e.g., exosomes encapsulating STING agonists. The immune response may be an IEN? response or activation of myeloid dendritic cells (mDCs). Also provided herein are methods of modulating an immune response that does not induce systemic inflammation via administration of exosomes encapsulating STING agonists.Type: ApplicationFiled: March 22, 2019Publication date: October 21, 2021Inventors: Su Chul JANG, Chang Ling SIA, Nuruddeen D. LEWIS, Rane A. HARRISON, Raymond J. MONIZ, Sriram SATHYANARAYANAN, Douglas E. WILLIAMS, Kyriakos ECONOMIDES
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Publication number: 20210290556Abstract: The instant application describes improved methods and compositions for the systemic delivery of therapeutic exosomes to a subject in need thereof. In certain embodiments, the current invention reduces the amount of exosomes delivered to liver, spleen and combinations thereof to allow greater distribution to other areas of the body such as, but not limited to, the brain, pancreas, lung, kidney, muscle. In certain embodiments, the methods involve the injection of one or multiple doses of non-therapeutic exosomes prior to the injection of a suitable therapeutic dose of exosomes with a therapeutic payload. Also included are methods to improve immune clearance of exosomes in subjects by inhibiting phagocytosis.Type: ApplicationFiled: August 21, 2017Publication date: September 23, 2021Applicant: Codiak BioSciences, Inc.Inventors: Douglas E. Williams, John D. Kulman
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Publication number: 20210262931Abstract: Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of light scattering of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.Type: ApplicationFiled: June 21, 2019Publication date: August 26, 2021Applicant: Codiak BioSciences, Inc.Inventors: Young Jun CHOI, Damian J. HOUDE, Delai CHEN, Douglas E. WILLIAMS
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Publication number: 20210093568Abstract: Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.Type: ApplicationFiled: September 8, 2020Publication date: April 1, 2021Inventors: Aaron R. NOYES, Michael P. MERCALDI, Kathryn E. GOLDEN, Raymond W. BOURDEAU, Michael F. DOHERTY, Konstantin KONSTANTINOV, Douglas E. WILLIAMS
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Publication number: 20200407725Abstract: Disclosed herein are compositions and methods comprising extracellular vesicles comprising nucleic acid that target genes, leading to macrophage polarization of tumor associated macrophages. In certain embodiments, disclosed herein are methods and compositions for increasing macrophage polarization for the treatment of cancer.Type: ApplicationFiled: February 12, 2019Publication date: December 31, 2020Applicant: CODIAK BIOSCIENCES, INC.Inventors: Sriram SATHYANARAYANAN, Douglas E. WILLIAMS, Dalia BURZYN, Adam Thomas BOUTIN, Sushrut KAMERKAR
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Publication number: 20200407419Abstract: Disclosed herein are extracellular vesicles comprising an immunomodulating component. Also provided are methods for producing the extracellular vesicles and methods for using the extracellular vesicles for treating cancer, GvHD, and autoimmune diseases.Type: ApplicationFiled: July 6, 2020Publication date: December 31, 2020Applicant: Codiak BioSciences, Inc.Inventors: Nuruddeen D. LEWIS, Yu ZHOU, Sriram SATHYANARAYANAN, John KULMAN, Douglas E. WILLIAMS, Leonid A. GAYDUKOV, Ke XU, Shelly MARTIN