Abstract: The disclosure provides methods, systems and related software, for automated or semi-automated sorting and/or isolating cells with visually distinguishable phenotypes. In some embodiments, the methods comprise providing a plurality cells with a photo-activatable detectable marker in their respective nuclei. The plurality of cells are imaged and, based on the image, the status for one or more visually identifiable phenotypes are determined. Cells determined to have the desired phenotype status are specifically exposed to a light wavelength for a time sufficient to uniquely activate the photo-activatable detectable marker in the individual cells with the desired phenotype status. The cells are then sorted on the basis of the activated detectable marker. The disclosure also provides methods for preparation and isolation of nuclei from fixed, adherent cells for analysis.

Abstract: The disclosure provides compositions and methods for suppressing off-target editing guide RNA-nuclease complexes. The disclosed strategies incorporate use of catalytically inactive truncated guide RNA/nuclease complexes to shield off-target editing. In some embodiments, the disclosure provides a method of inhibiting off-target cleavage of DNA by a first guide RNA-endonuclease complex by contacting the DNA with a second guide RNA-endonuclease complex that comprises a second guide RNA corresponding to the off-target site but with a recognition sequence of 16 or fewer nucleotides. In another aspect, the disclosure provides a method for preventing cleavage of DNA after editing and subsequent homology-directed repair (HDR) by contacting the repaired DNA with a guide RNA-endonuclease complex that comprises a guide RNA with a guide RNA corresponding to the repaired sequence but with a recognition sequence of 16 or fewer nucleotides. Additional methods, compositions, and kits are also provided.

Abstract: An apparatus comprises an optics assembly and a plate. The optics assembly configured to focus light from an EUV source onto a reticle or sensor. The plate has an opening to allow the EUV light to pass through disposed between the optics assembly and the reticle or sensor. The plate is cooled to a temperature less than that of the reticle or sensor. The plate is engineered to balance out heat absorbed from the reticle or sensor with heat absorbed by the plate. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.

Abstract: A new and useful portable adjustable multi-purpose exercise device which is collapsible when not in use, relatively inexpensive, allows for a multitude of exercises to be performed for every major part of the body and focuses on exercises which implement the user's body weight as resistance. The device essentially comprises two triangular support frames and an attached stabilizer bar, which when assembled yields a pyramidal prismatic structure upon which the user performs a variety of exercises. The configuration of the device changes as the user optionally places an exercise grip bar in various receiving grooves. This device is believed to be useful in the home setting, whereby the individual user may obtain a full work-out without the need for larger home gym systems. A device according to the invention is also believed to be favorable for use in large gym environments, hospital therapy rooms, classes and other fitness-related facilities.

Abstract: An image sensor is disclosed that includes a solid state semiconductor imager having a metallized catch pad, a collimator having a metallized layer that faces a sensor anode, the metallized layer joined with the metallized catch pad to form a metal bond between the solid state semiconductor imager and the collimator. Methods of making the joined solid state semiconductor imager and collimator assembly are also disclosed.

Abstract: An apparatus comprises an optics assembly and a plate. The optics assembly configured to focus light from an EUV source onto a reticle or sensor. The plate has an opening to allow the EUV light to pass through disposed between the optics assembly and the reticle or sensor. The plate is cooled to a temperature less than that of the reticle or sensor. The plate is engineered to balance out heat absorbed from the reticle or sensor with heat absorbed by the plate. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.

Abstract: A new and useful portable adjustable multi-purpose exercise device which is collapsible when not in use, relatively inexpensive, allows for a multitude of exercises to be performed for every major part of the body and focuses on exercises which implement the user's body weight as resistance. The device essentially comprises two triangular support frames and an attached stabilizer bar, which when assembled yields a pyramidal prismatic structure upon which the user performs a variety of exercises. The configuration of the device changes as the user optionally places an exercise grip bar in various receiving grooves. This device is believed to be useful in the home setting, whereby the individual user may obtain a full work-out without the need for larger home gym systems. A device according to the invention is also believed to be favorable for use in large gym environments, hospital therapy rooms, classes and other fitness-related facilities.

Abstract: An image sensor is disclosed that includes a solid state semiconductor imager having a metallized catch pad, a collimator having a metallized layer that faces a sensor anode, the metallized layer joined with the metallized catch pad to form a metal bond between the solid state semiconductor imager and the collimator. Methods of making the joined solid state semiconductor imager and collimator assembly are also disclosed.

Abstract: An apparatus that provides multi-mode digital input to computers, tablets, personal digital assistants, global positioning satellite systems, and other such devices. Digital information can be transferred via passive inputs such as resistive or capacitive touch using a human finger or a stylus, via passive acoustic surface wave or electro-optical devices, via active technology such as inductive and RF inputs, and via active wired inputs such as a mouse or a touch pad. The preferred input mode can be selected by an operator, or simultaneous input modes can be sensed, differentiated, and prioritized by the apparatus.

Abstract: A fixed body wide field-of-view conformal antenna array suitable for broadband precision direction finding on missile platforms. The array is configured as multiple sub-arrays of spiral antennas that cover particular regions within the desired field-of-view of the entire array. A lower cost, more reliable and more accurate direction finding solution for missile needs is provided, primarily by the elimination of conventional radomes and antenna gimbal structures. The array can be configured to include multi-mode sensors.

Abstract: A fixed body wide field-of-view conformal antenna array suitable for broadband precision direction finding on missile platforms. The array is configured as multiple sub-arrays of spiral antennas that cover particular regions within the desired field-of-view of the entire array. A lower cost, more reliable and more accurate direction finding solution for missile needs is provided, primarily by the elimination of conventional radomes and antenna gimbal structures. The array can be configured to include multi-mode sensors.

Abstract: A fixed body wide field-of-view conformal antenna array suitable for broadband precision direction finding on missile platforms. The array is configured as multiple sub-arrays of spiral antennas that cover particular regions within the desired field-of-view of the entire array. A lower cost, more reliable and more accurate direction finding solution for missile needs is provided, primarily by the elimination of conventional radomes and antenna gimbal structures. The array can be configured to include multi-mode sensors.

Abstract: This disclosure relates to apparatus for washing trays and/or buggies, and includes a housing having means therein for conveying buggies from an entrance opening to an exit opening through respective washing and rinse zones beneath which are respective wash water and rinse water collecting reservoirs, a plurality of nozzles in the washing zone disposed in an inverted generally U-shaped configuration for directing high pressure streams of wash water downwardly and across the washing zone, a plurality of nozzles disposed at an upper area of the rinse zone for directing rinse water generally downwardly through the rinse zone, and means responsive to detecting means for detecting the level of collected wash water and rinse water in the respective washing and rinse reservoirs for adding make-up water to the washing and rinse nozzles.

Abstract: This disclosure relates to apparatus for and a novel method of washing trays and/or buggies, and includes a housing having means therein for conveying buggies from an entrance opening to an exit opening through respective washing and rinse zones beneath which are respective wash water and rinse water collecting reservoirs, a plurality of nozzles in the washing zone disposed in an inverted generally U-shaped configuration for directing high pressure streams of wash water downwardly and across the washing zone, a plurality of nozzles disposed at an upper area of the rinse zone for directing rinse water generally downwardly through the rinse zone, and means responsive to detecting means for detecting the level of collected wash water and rinse water in the respective washing and rinse reservoirs for adding make-up water to the washing and rinse nozzles.