Abstract: Embodiments of the present invention relate to a rapid, automated, and objective method for using oculomotor measures to determine whether a person is being truthful or deceitful. More specifically, embodiments of the present invention measure a plurality of oculomotor and behavioral dependent measures while a subject reads and responds to written items that are both related and unrelated to a suspected crime. The oculomotor and behavioral measures may include pupil diameter, response times, the number of fixations during reading, the time spent reading and rereading items, and the rate of eye blinks on the current and subsequent item. Several behavioral and oculomotor measures were diagnostic of deception, and a weighted combination of four of those variables correctly classified 84% of guilty and 89% of innocent subjects.