Abstract: A method for spectrographically providing a chemical profile of mammalian cells in vitro using capillary (10) electrophoresis is described. The method enables rapid determination of the chemicals, especially human cell metabolites, in the case of diabetes. The method is rapid, efficient and reproducible and enables the diagnosis of the progress of a disease at the cellular level.

Abstract: A method for spectrographically providing a chemical profile of mammalian cells in vitro using capillary (10) electrophoresis is described. The method enables rapid determination of the chemicals, especially human cell metabolites, in the case of diabetes. The method is rapid, efficient and reproducible and enables the diagnosis of the progress of a disease at the cellular level.

Abstract: A test method and test kit for determining a risk of diabetic complications based upon abnormal aldose reductase genetic material expression is described. Cells isolated from a patient which exhibit elevated levels of aldose reductase genetic material expression at pathophysiologic levels of glucose (about 20 mM) which can occur commonly in the cells of diabetic patients are evaluated based upon a level of expression of DNA or RNA in the cells with the glucose at the pathophysiologic level. The cells can be used to isolate DNA or RNA for a probe which detects the abnormal aldose reductase gene expression. The method can be used to determine when particular aldose reductase inhibitors can be effective for a particular patient.