Patents by Inventor Douglas Prasher

Douglas Prasher has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Green fluorescent protein
    Patent number: 6146826
    Abstract: This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green fluorescent protein operatively linked to a DNA sequence encoding the green fluorescent protein. This invention also provides living organisms which comprise the above-described cell. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green fluorescent protein; b) culturing the introduced cells under conditions permitting expression of the green fluorescent protein and the protein of interest; and c) selecting the cultured cells which express green fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green fluorescent protein.
    Type: Grant
    Filed: June 18, 1996
    Date of Patent: November 14, 2000
    Assignees: The Trustees of Columbia University in the City of New York, Woods Hole Oceanographic Institution
    Inventors: Martin Chalfie, Douglas Prasher
  • Recombinant DNA vectors capable of expressing apoaequorin
    Patent number: 5798441
    Abstract: A gene which codes for the protein apoaequorin is disclosed along with recombinant DNA vectors containing this gene. Homogeneous peptides having the bioluminescence properties of natural, mixed apoaeqorin are also disclosed.
    Type: Grant
    Filed: November 29, 1994
    Date of Patent: August 25, 1998
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Milton J. Cormier, Douglas Prasher
  • Recombinant DNA vectors capable of expressing apoaequorin
    Patent number: 5766941
    Abstract: A gene which codes for the protein apoaequorin is disclosed along with recombinant DNA vectors containing this gene. Homogeneous peptides having the bioluminescence properties of natural, mixed apoaeqorin are also disclosed.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: June 16, 1998
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Milton J. Cormier, Douglas Prasher
  • Recombinant DNA vectors capable of expressing apoaequorin
    Patent number: 5744579
    Abstract: A gene which codes for the protein apoaequorin is disclosed along with recombinant DNA vectors containing this gene. Homogeneous peptides having the bioluminescence properties of natural, mixed apoaequorin are also disclosed.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: April 28, 1998
    Assignee: University of Georgia Research Foundation
    Inventors: Milton J. Cormier, Douglas Prasher
  • Modified apoaequorin having increased bioluminescent activity
    Patent number: 5541309
    Abstract: The present invention encompasses modified apoaequorin nucleotide and amino acid sequences capable of emitting light in the presence of luciferin and a light-triggering cation such as Ca.sup.2+, which possess bioluminescent activity greater than unmodified apoaequorin and methods of use therefore.
    Type: Grant
    Filed: October 28, 1994
    Date of Patent: July 30, 1996
    Assignee: Woods Hole Oceanographic Institution (WHOI)
    Inventor: Douglas Prasher
  • Uses of green-fluorescent protein
    Patent number: 5491084
    Abstract: This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green-fluorescent protein operatively linked to a DNA sequence encoding the green-fluorescent protein. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a. introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green-fluorescent protein; b. culturing the introduced cells in conditions permitting expression of the green-fluorescent protein and the protein of interest; and c. selecting the cultured cells which express green-fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green-fluorescent protein.
    Type: Grant
    Filed: September 10, 1993
    Date of Patent: February 13, 1996
    Assignees: The Trustees of Columbia University in the City of New York, Woods Hole Oceanographic Institution
    Inventors: Martin Chalfie, Douglas Prasher
  • Recombinant DNA vectors capable of expressing apoaequorin
    Patent number: 5422266
    Abstract: A gene which codes for the protein apoaequorin is disclosed alone with recombinant DNA vectors containing this gene. Homogeneous peptides having the bioluminescence properties of natural, mixed apoaeqorin are also disclosed.
    Type: Grant
    Filed: October 9, 1992
    Date of Patent: June 6, 1995
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Milton J. Cormier, Douglas Prasher
  • Modified apoaequorin having increased bioluminescent activity
    Patent number: 5360728
    Abstract: The present invention encompasses modified apoaequorin nucleotide and amino acid sequences capable of emitting light in the presence of luciferin and a light-triggering cation such as Ca.sup.2+, which possess bioluminescent activity greater than unmodified apoaequorin and methods of use therefore.
    Type: Grant
    Filed: December 1, 1992
    Date of Patent: November 1, 1994
    Assignee: Woods Hole Oceanographic Institution (W.H.O.I.)
    Inventor: Douglas Prasher