Abstract: In one or more embodiments, a method includes selecting a cemented carbide substrate from a plurality of cemented carbide substrates in a substrate inventory. Each of the plurality of cemented carbide substrates have a substantially planar top surface. The method also includes emitting a plurality of laser pulses from a laser towards at least the substantially planar top surface of the cemented carbide substrate to ablate selected regions of the cemented carbide substrate thereby forming the cemented carbide substrate into a selected shape.

Abstract: An Identity and Access Management Service implements persistent source values PSVs) for assumed identities. A source value (e.g., an original identifier of an entity) is persisted across assumed identities, facilitating identification of entities (users or applications) responsible for actions taken by the assumed (e.g., alternative) identities. The Manager receives a request to assume an identity. The request includes the entities current credentials and a PSV. The current credentials are authenticated and a persistent source value policy may be relied on to determine whether and/or how to grant the assumed identity. The PSV may be copied from credentials in the request in order to be included in the credentials for the requested identity that the Manager provides in response to the request. Use of the requested credentials, including the PSV, to access services or resources may be logged, the logs including the PSV from the request to assume the identity.

Abstract: An Identity and Access Management Service implements persistent source values PSVs) for assumed identities. A source value (e.g., an original identifier of an entity) is persisted across assumed identities, facilitating identification of entities (users or applications) responsible for actions taken by the assumed (e.g., alternative) identities. The Manager receives a request to assume an identity. The request includes the entities current credentials and a PSV. The current credentials are authenticated and a persistent source value policy may be relied on to determine whether and/or how to grant the assumed identity. The PSV may be copied from credentials in the request in order to be included in the credentials for the requested identity that the Manager provides in response to the request. Use of the requested credentials, including the PSV, to access services or resources may be logged, the logs including the PSV from the request to assume the identity.

Abstract: In one example in accordance with the present disclosure, a printing system is described. The printing system includes a media sensor to detect a presence of media at a particular point within the printing system. A stepper motor moves media through the printing system. A controller 1) monitors, for at least one pass of the media, a number of steps of the stepper motor to pass the media between the media sensor and a print position, 2) stores the number of steps of the stepper motor in a memory device, and 3) adjusts operation of subsequent passes of the media based on stored number of steps. A memory device of the printing system stores the number of steps of the stepper motor, for at least one pass of the media.

Abstract: In one example in accordance with the present disclosure, a printing system is described. The printing system includes a media sensor to detect a presence of media at a particular point within the printing system. A stepper motor moves media through the printing system. A controller 1) monitors, for at least one pass of the media, a number of steps of the stepper motor to pass the media between the media sensor and a print position, 2) stores the number of steps of the stepper motor in a memory device, and 3) adjusts operation of subsequent passes of the media based on stored number of steps. A memory device of the printing system stores the number of steps of the stepper motor, for at least one pass of the media.

Abstract: A method and system of processing seismic data is presented. The method may include, for each of a plurality of seismic traces, generating a respective intermediate set of reflectivity coefficients and a partial deconvolution of an estimated wavelet from the respective seismic trace. The method may also include decomposing a model into a plurality of orthogonal components, and projecting each of a plurality of eigenvectors corresponding to one of the orthogonal components onto intermediate reflectivity coefficients corresponding with all of the plurality of seismic traces at each of a plurality of times to generate a plurality of eigen-coefficients associated with each of the plurality of times. The eigen-coefficients may be used to generate a plurality of basis coefficients, which may then be used to generate a respective updated set of reflectivity coefficients for each of the seismic traces.

Abstract: A molecular detection assay including treating a biological sample directly with a bisulphite agent under conditions that allow cell disruption and nucleic acid treatment; removing the bisulphite agent from the treated sample; and detecting a target nucleic acid in the treated sample.

Abstract: A method and system of processing seismic data is presented. The method may include, for each of a plurality of seismic traces, generating a respective intermediate set of reflectivity coefficients and a partial deconvolution of an estimated wavelet from the respective seismic trace. The method may also include decomposing a model into a plurality of orthogonal components, and projecting each of a plurality of eigenvectors corresponding to one of the orthogonal components onto intermediate reflectivity coefficients corresponding with all of the plurality of seismic traces at each of a plurality of times to generate a plurality of eigen-coefficients associated with each of the plurality of times. The eigen-coefficients may be used to generate a plurality of basis coefficients, which may then be used to generate a respective updated set of reflectivity coefficients for each of the seismic traces.

Abstract: A molecular detection assay including treating a biological sample directly with a bisulphite agent under conditions that allow cell disruption and nucleic acid treatment; removing the bisulphite agent from the treated sample; and detecting a target nucleic acid in the treated sample.

Abstract: The invention relates to the use of enzymes for copying or amplifying bisulphite modified or treated nucleic acids, wherein the enzymes are more effective in copying or amplifying the nucleic acid compared with native Taq polymerase under substantially the same conditions.

Abstract: A lubrication system is provided for delivering a lubricant to a plurality of nozzles. The lubrication system includes a reservoir for the lubricant; a pump fluidly coupled to the reservoir and configured to remove a flow of the lubricant from the reservoir; a lubrication sensor positioned between the pump and the plurality of nozzles and configured to detect a blockage in the plurality of nozzles and to generate a blockage signal when the blockage is detected; and an indicator coupled to the lubrication sensor and configured to generate a warning based on the blockage signal from the lubrication sensor.

Abstract: A method for detecting a microorganism comprising reducing the complexity of a microbial genome or microbial nucleic acid by generating a simplified form of the microbial genome or microbial nucleic acid in which substantially all of the positions naturally occupied by cytosines are occupied by uracil or thymine; and assaying for a microbial specific nucleic acid in the simplified form of the microbial genome or microbial nucleic acid, wherein presence of the microbial specific nucleic acid is indicative of the microorganism.

Abstract: The invention is directed to a method for isothermal DNA amplification comprising providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing a non-regular base, a second primer at least partially complementary to a region of DNA and containing a non- regular base, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises a non-regular base in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near the non-regular base; and amplifying the DNA substantially without thermal cycling.

Abstract: A lubrication system is provided for delivering a lubricant to a plurality of nozzles.

Abstract: The invention relates to an assay for determining a health state of a subject using a combination of detecting the presence of a virus and detecting the presence of a genomic target or marker indicative of a health state.

Abstract: Damping systems and engines including the damping systems are provided. In one embodiment, and by way of example only, the damping system includes a rotor, a bearing assembly mounted to the rotor, a spring cage disposed around the bearing assembly, an axially-extending housing surrounding the spring cage, a first squeeze film damper formed between the bearing assembly and the spring cage, and a second squeeze film damper formed between the spring cage and the axially-extending housing.

Abstract: Methods for treating nucleic acid including: (a) providing an alkali environment to a nucleic acid sample; (b) reacting the nucleic acid sample with a bisulphite reagent and incubating the reaction so as to form a treated nucleic acid sample where methylated nucleotides in the nucleic acid sample remain unchanged while unmethylated nucleotides are converted to another form; (c) removing unwanted reagents or diluents from the treated nucleic acid sample; and (d) carrying out de-sulphonation of the precipitated treated nucleic acid at a temperature from 70° C. to 95° C. by adjusting the precipitated treated nucleic acid to a pH of between 10 and less than 12.5 to remove sulphonate groups present on the treated nucleic acid and obtain a nucleic acid sample substantially free of sulphonate groups.

Abstract: A method for isothermal DNA amplification comprising: providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing Xanthosine, a second primer at least partially complementary to a region of DNA and containing Xanthosine, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises Xanthosine in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near Xanthosine; and amplifying the DNA substantially without thermal cycling.

Abstract: A tool disposable within a well pipe, such as for detecting a freepoint of a well pipe, includes an electromagnet capable of inducing a magnetic field within a wall of a well pipe. The tool includes a Barkhausen noise sensing device capable of sensing magnetic Barkhausen noise in response to the electromagnet inducing the magnetic field within the wall of the well pipe. The tool may be moved along the well pipe so as make two passes of the tool along the well pipe, with one pass being performed while the well pipe is less stressed and the other pass being performed while the well pipe is more stressed, with the output of the tool during a first pass being compared to the output of the tool during a second pass to determine the location of the freepoint of the well pipe.

Abstract: A portable swing seat comprises a flexible seat portion, a support assembly having a pair of flexible support straps affixed to the seat portion, a spreader extending between the support straps distally from the seat portion, and an attachment strap affixed to the support assembly. The attachment strap is attachable to an object whereby when in use the attachment strap is affixed to an object above the spreader and an individual may be supported while sitting on the seat portion with the support straps extending upward from the seat portion such that the spreader maintains spacing between the support straps.