Abstract: A multifunctional thermal exposure indicator can provide immediate reversible indications of overtemperature as well as irreversible indications of excessive thermal loading. Additionally, the multifunctional thermal exposure indicator can provide semi-reversible indications of undertemperature thermal loading, such as exposure to temperatures below the freezing point of water.

Abstract: Methods and reagent for determining the presence and/or for quantifying the amount of a target nucleic acid sequences in a sample are provided. In some aspects, the methods comprise performing a melt analysis by detecting a signal from a hairpin probe that forms only in the presence of the target nucleic acid.

Abstract: This disclosure relates to apparatus and methods for thermally cycling a sample. Particular embodiments comprise a first pivot arm configured to pivot around a first pivot axis; a second pivot arm configured to pivot around a second pivot axis; a first thermal mass and a second thermal mass coupled to the first pivot arm; and a third thermal mass and a fourth thermal mass coupled to the second pivot arm, wherein the first and third thermal masses are proximal to the sample when the first and second pivot arms are in a first position, and the second and fourth thermal masses are proximal to the sample when the first and second pivot arms are in a second position.

Abstract: Methods and reagent for determining the presence and/or for quantifying the amount of a target nucleic acid sequences in a sample are provided. In some aspects, the methods comprise performing a melt analysis by detecting, a signal from a probe at a temperature that is lower than the Tm of the probe and a signal at a temperature that is higher than the Tm of the probe, without detecting a signal at the Tm of the probe.

Abstract: Methods and reagent for determining the presence and/or for quantifying the amount of a target nucleic acid sequences in a sample are provided. In some aspects, the methods comprise performing a melt analysis by detecting, a signal from a probe at a temperature that is lower than the Tm of the probe and a signal at a temperature that is higher than the Tm of the probe, without detecting a signal at the Tm of the probe.

Abstract: This disclosure relates to apparatus and methods for thermally cycling a sample. Particular embodiments comprise a first pivot arm configured to pivot around a first pivot axis; a second pivot arm configured to pivot around a second pivot axis; a first thermal mass and a second thermal mass coupled to the first pivot arm; and a third thermal mass and a fourth thermal mass coupled to the second pivot arm, wherein the first and third thermal masses are proximal to the sample when the first and second pivot arms are in a first position, and the second and fourth thermal masses are proximal to the sample when the first and second pivot arms are in a second position.

Abstract: Method of nucleic acid amplifying are provided. In some aspects, methods involve amplifying at least two different target nucleic acids in a reaction mixture.

Abstract: Methods and reagent for determining the presence and/or for quantifying the amount of a target nucleic acid sequences in a sample are provided. In some aspects, the methods comprise performing a melt analysis by detecting, a signal from a probe at a temperature that is lower than the Tm of the probe and a signal at a temperature that is higher than the Tm of the probe, without detecting a signal at the Tm of the probe.

Abstract: Methods for querying biological samples to detect genetic mutations, particularly insertions and deletions, by co-amplification of a gene of interest in conjunction with a paralogous gene. When the gene of interest and the corresponding paralogous gene are selected from the CYP450 family, the resulting ratios may predict how a particular patient metabolizes certain prescription drugs.

Abstract: Methods for querying biological samples to detect genetic mutations, particularly insertions and deletions, by co-amplification of a gene of interest in conjunction with a paralogous gene. When the gene of interest and the corresponding paralogous gene are selected from the CYP450 family, the resulting ratios may predict how a particular patient metabolizes certain prescription drugs.