Patents by Inventor Douwe Van Sinderen
Douwe Van Sinderen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11674117Abstract: The present invention relates to a starter culture comprising at least two Streptococcus thermophilus strains, wherein a first and a second Streptococcus thermophilus strain are chosen from RGP group 1, RGP group 2, RGP group 3 and RGP group 4, with the proviso that the first and second Streptococcus thermophilus strains do not belong to the same RGP group.Type: GrantFiled: December 14, 2017Date of Patent: June 13, 2023Assignee: DSM IP ASSETS B.V.Inventors: Roelof Hendrik Matthijs Kouwen, Douwe Van Sinderen, Brian McDonnell, Pieter Emiel Verloren Van Themaat, Jennifer Mahony
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Publication number: 20200377961Abstract: The multiple embodiments described herein comprise the genome of a probiotic Bifidobacterium longum bifidobacteria strain and genes encoded by the genome. Various novel Bifidobacterium longum are described.Type: ApplicationFiled: May 12, 2020Publication date: December 3, 2020Applicant: PrecisionBiotics Group LimitedInventors: Douwe Van Sinderen, Jun Xu, Wenzhu Zhao, Raymond A. Grant, Yuli Song, Charles Bascom, Duane Larry Charbonneau, Liam O'Mahony
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Publication number: 20200317738Abstract: The invention describes peptide, and proteins and fusion proteins comprising the peptides, that are capable of increasing the proliferation of cells, especially epithelial cells in the mammalian gastrointestinal tract. The peptides comprise the hydrophilic domain of the TadE subunit of the Tad pilus of Bifidobacterium, especially the pilus encoded by the Tad2003 gene cluster identified in Bifidobacterium breve UCC2003, and functional fragments of the hydrophilic domain. The invention informs new treatments for diseases and conditions that involve attenuated epithelial cell proliferation and growth, such as inflammatory conditions of the gastrointestinal tract and trauma of the skin including burns and other wounds. The invention also informs treatments of newborn babies to promote proliferation and growth of gut epithelial tissue by administration of agents comprising the peptide of the invention in the form of infant formula.Type: ApplicationFiled: May 26, 2017Publication date: October 8, 2020Inventors: Mary O'Connell Motherway, Douwe Van Sinderen, Fergus Shanahan, Aileen Houston
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Patent number: 10689719Abstract: The multiple embodiments described herein comprise the genome of a probiotic Bifidobacterium longum bifidobacteria strain and genes encoded by the genome. Various novel Bifidobacterium longum are described.Type: GrantFiled: August 23, 2017Date of Patent: June 23, 2020Assignee: PrecisionBiotics Group LimitedInventors: Douwe Van Sinderen, Jun Xu, Wenzhu Zhao, Raymond A. Grant, Yuli Song, Charles Bascom, Duane Larry Charbonneau, Liam O'Mahony
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Patent number: 10538818Abstract: A kit useful for determining the phage susceptibility of one or more strains of Lactococcus lactis is described. The disclosure also describes methods for formulation of mixed defined starter cultures using strains from different phage sensitivity groups.Type: GrantFiled: March 13, 2014Date of Patent: January 21, 2020Assignee: DSM IP ASSETS B.V.Inventors: Douwe Van Sinderen, Finn Vogensen, Jennifer Mahony, Witold Kot, Stuart Ainsworth
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Patent number: 10519516Abstract: The present invention relates to a kit suitable for detection of Streptococcus thermophilus infecting phages, said kit comprising: (a) a first primer pair suitable to generate an amplicon characteristic for a 987 phage; and (b) a second primer pair suitable to generate an amplicon characteristic for a 5093 phage. Further, the present invention relates to a method for detecting the presence of Streptococcus thermophilus infecting phages in a sample.Type: GrantFiled: May 8, 2017Date of Patent: December 31, 2019Assignee: DSM IP ASSETS B.V.Inventors: Roelof Hendrik Matthijs Kouwen, Douwe Van Sinderen, Brian McDonnell
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Patent number: 10519519Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.Type: GrantFiled: July 2, 2018Date of Patent: December 31, 2019Assignee: DSM IP ASSETS B.V.Inventors: Roelof Hendrik Matthijs Kouwen, Pim Van Hee, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
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Publication number: 20190367866Abstract: The present invention relates to a starter culture comprising at least two Streptococcus thermophilus strains, wherein a first and a second Streptococcus thermophilus strain are chosen from RGP group 1, RGP group 2, RGP group 3 and RGP group 4, with the proviso that the first and second Streptococcus thermophilus strains do not belong to the same RGP group.Type: ApplicationFiled: December 14, 2017Publication date: December 5, 2019Inventors: Roelof Hendrik Matthijs KOUWEN, Douwe VAN SINDEREN, Brian MCDONNELL, Pieter Emiel VERLOREN VAN THEMAAT, Jennifer MAHONY
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Patent number: 10244771Abstract: The present invention provides a method for the construction of a bacteriophage-insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance mechanism but based on another mechanism. The method provides a protocol by which this can be achieved by inactivating one or more of the CRISPR systems present in a given strain. The invention also provides the bacteriophage-insensitive mutants as well as their use in a process for making a dairy product.Type: GrantFiled: July 23, 2015Date of Patent: April 2, 2019Assignee: DSM IP ASSETS B.V.Inventors: Roelof Hendrik Matthijs Kouwen, Laurens Leendert Hanemaaijer, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
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Patent number: 10184127Abstract: The present invention concerns a method for genetically transforming a Bifidobacterium strain comprising a step of methylation of a shuttle vector in an E. coli or a Gram-positive bacterium strain by two type II DNA methyltransferases from a Bifidobacterium: a methyltransferase enzyme that methylates the adenine base at position 4 of the nucleotide sequence RTCAGG and a methyltransferase enzyme that methylates the cytosine base at position 4 of the nucleotide sequence GGWCC. The present invention also concerns genetic tools and culture media useful for carrying out said method.Type: GrantFiled: December 2, 2013Date of Patent: January 22, 2019Assignees: Compagnie Gervais Danone, University College Cork—National University of Ireland, CorkInventors: Douwe van Sinderen, Mary O'Connell Motherway, Debbie Watson, Tamara Smokvina, Peggy Garault
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Publication number: 20180305777Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.Type: ApplicationFiled: July 2, 2018Publication date: October 25, 2018Inventors: Roelof Hendrik Matthijs KOUWEN, Pim Van Hee, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
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Patent number: 10041135Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.Type: GrantFiled: February 20, 2015Date of Patent: August 7, 2018Assignee: DSM IP ASSETS B.V.Inventors: Roelof Hendrik Matthijs Kouwen, Pim Van Hee, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
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Publication number: 20180016647Abstract: The multiple embodiments described herein comprise the genome of a probiotic Bifidobacterium longum bifidobacteria strain and genes encoded by the genome. Various novel Bifidobacterium longum are described.Type: ApplicationFiled: August 23, 2017Publication date: January 18, 2018Inventors: Douwe Van Sinderen, Jun Xu, Wenzhu Zhao, Raymond A. Grant, Yuli Song, Charles Bascom, Duane Larry Charbonneau, Liam O'Mahony
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Publication number: 20170327912Abstract: The present invention relates to a kit suitable for detection of Streptococcus thermophilus infecting phages, said kit comprising: (a) a first primer pair suitable to generate an amplicon characteristic for a 987 phage; and (b) a second primer pair suitable to generate an amplicon characteristic for a 5093 phage. Further, the present invention relates to a method for detecting the presence of Streptococcus thermophilus infecting phages in a sample.Type: ApplicationFiled: May 8, 2017Publication date: November 16, 2017Inventors: Roelof Hendrik Matthijs Kouwen, Douwe Van Sinderen, Brian McDonnell
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Patent number: 9771624Abstract: The multiple embodiments described herein comprise the genome of a probiotic Bifidobacterium longum bifidobacteria strain and genes encoded by the genome. Various novel Bifidobacterium longum are described.Type: GrantFiled: November 11, 2009Date of Patent: September 26, 2017Assignee: The Procter & Gamble CompanyInventors: Douwe Van Sinderen, Jun Xu, Wenzhu (Steven) Zhao, Raymond A. Grant, Song Yuli, Charles Bascom, Duane Larry Charbonneau, Liam O'Mahony
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Publication number: 20170218468Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.Type: ApplicationFiled: February 20, 2015Publication date: August 3, 2017Inventors: Roelof Hendrik Matthijs KOUWEN, Pim VAN HEE, Douwe VAN SINDEREN, Brian MCDONNELL, Jennifer MAHONY
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Publication number: 20170196233Abstract: The present invention provides a method for the construction of a bacteriophage-insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance mechanism but based on another mechanism. The method provides a protocol by which this can be achieved by inactivating one or more of the CRISPR systems present in a given strain. The invention also provides the bacteriophage-insensitive mutants as well as their use in a process for making a dairy product.Type: ApplicationFiled: July 23, 2015Publication date: July 13, 2017Inventors: Roelof Hendrik Matthijs KOUWEN, Laurens Leendert HANEMAAIJER, Douwe VAN SINDEREN, Brian MCDONNELL, Jennifer MAHONY
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Publication number: 20160304885Abstract: The present invention concerns a method for genetically transforming a Bifidobacterium strain comprising a step of methylation of a shuttle vector in an E. coli or a Gram-positive bacterium strain by two type II DNA methyltransferases from a Bifidobacterium: a methyltransferase enzyme that methylates the adenine base at position 4 of the nucleotide sequence RTCAGG and a methyltransferase enzyme that methylates the cytosine base at position 4 of the nucleotide sequence GGWCC. The present invention also concerns genetic tools and culture media useful for carrying out said method.Type: ApplicationFiled: December 2, 2013Publication date: October 20, 2016Applicants: Compagnie Gervais Danone, University College Cork - National University of Ireland, CorkInventors: Douwe van Sinderen, Mary O'Connell Motherway, Debbie Watson, Tamara Smokvina, Peggy Garault
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Publication number: 20160017408Abstract: A kit useful for determining the phage susceptibility of one or more strains of Lactococcus lactis is described. The disclosure also describes methods for formulation of mixed defined starter cultures using strains from different phage sensitivity groups.Type: ApplicationFiled: March 13, 2014Publication date: January 21, 2016Inventors: Douwe VAN SINDEREN, Finn VOGENSEN, Jennifer MAHONY, Witold KOT, Stuart AINSWORTH
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Publication number: 20150359909Abstract: The current invention relates to the use of a bacterial species in the preparation of a composition adapted for oral administration for the delivery of an agent to a site in the body. The site in the body may be an organ or a tumour site. The bacterial species is a food grade, non-pathogenic, gram-positive bacteria capable of anaerobic growth.Type: ApplicationFiled: July 14, 2015Publication date: December 17, 2015Inventors: Gerald O'SULLIVAN, Mark TANGNEY, Douwe VAN SINDEREN, Michelle CRONIN