Patents by Inventor Dries BRAEKEN
Dries BRAEKEN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12037568Abstract: A semiconductor cell culture device for three-dimensional cell culture comprises: a semiconductor material layer in which a cell culture portion of semiconductor material is defined, wherein the cell culture portion defines an area within the semiconductor material layer surrounded by semiconductor material, wherein the cell culture portion comprises a mesh structure having island structures being interconnected by bridge structures and defining through-pores between the island structures allowing for selective transport of cell constructs, cellular components, proteins or other large molecules through the semiconductor material layer and on opposite sides of the cell culture portion in the semiconductor material layer, and a supporting structure connected to the cell culture portion.Type: GrantFiled: December 19, 2020Date of Patent: July 16, 2024Assignees: IMEC VZW, Katholieke Universiteit LeuvenInventors: Aaron Delahanty, Dries Braeken, Alexandru Andrei, Peter Peumans, Carolina Mora Lopez, Veerle Reumers, Veronique Rochus, Bart Weekers
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Patent number: 11687031Abstract: A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity termType: GrantFiled: December 19, 2020Date of Patent: June 27, 2023Assignees: IMEC VZW, KATHOLIEKE UNIVERSITEIT LEUVENInventors: Zhenxiang Luo, Abdulkadir Yurt, Dries Braeken, Liesbet Lagae, Richard Stahl
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Patent number: 11668697Abstract: A device for analysis of cells comprises: an integrated circuit arrangement on a substrate; a dielectric layer formed above the integrated circuit arrangement; a microelectrode array layer formed above the dielectric layer, said microelectrode array layer comprising a plurality of individual electrodes, wherein each electrode is connected to the integrated circuit arrangement through a via in the dielectric layer; and wherein a plurality of longitudinal trenches in the dielectric layer and the microelectrode array layer are for stimulating cell growth on a surface of the deviceType: GrantFiled: June 24, 2019Date of Patent: June 6, 2023Assignees: IMEC VZW, KATHOLIEKE UNIVERSITEIT LEUVEN, KU LEUVEN R&DInventors: Dries Braeken, Veerle Reumers, Alexandru Andrei, Andrea Firrincieli, Thomas Pauwelyn
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Publication number: 20220333196Abstract: Systems and methods are provided for labelling nucleic acids in a cell of a cell culture or of a tissue. The systems and methods include contacting the cell culture or tissue with a first label and applying an electrical field to the cell, thereby increasing the permeability of the cell membrane, allowing the first label to be introduced into the cell. The cell culture or tissue can be disposed on a multielectrode array, and the electrical field can be applied by operating one or more electrodes of the array that are proximate to (e.g., beneath) the cell in order to control the location of the cell whose permeability is increased and/or to control the timing of such permeability increase.Type: ApplicationFiled: April 13, 2022Publication date: October 20, 2022Inventor: Dries Braeken
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Publication number: 20210371796Abstract: An electrode unit for performing electroporation of a biological cell is provided, including at least one electrode which can contact a cell. A stimulation unit and an impedance measurement device are connected to the cell, respectively to provide signals to provide cell electroporation and to measure the impedance of the electrode in contact with the cell. Further, a memory stores a predetermined value of an impedance parameter of the biological cell, and it is arranged to be readable by a comparing element. The comparing element is configured to compare the value stored in the memory with a value measured with the impedance measurement device, and to produce an adjustment signal to the stimulation unit, forming a feedback loop. The unit is further configured to apply a further electrical signal for providing electroporation of the cell upon receiving the adjustment signal from the comparing element.Type: ApplicationFiled: May 21, 2021Publication date: December 2, 2021Inventors: Bastien Duckert, Dries Braeken, Maarten Fauvart
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Publication number: 20210189310Abstract: A semiconductor cell culture device for three-dimensional cell culture comprises: a semiconductor material layer in which a cell culture portion of semiconductor material is defined, wherein the cell culture portion defines an area within the semiconductor material layer surrounded by semiconductor material, wherein the cell culture portion comprises a mesh structure having island structures being interconnected by bridge structures and defining through-pores between the island structures allowing for selective transport of cell constructs, cellular components, proteins or other large molecules through the semiconductor material layer and on opposite sides of the cell culture portion in the semiconductor material layer, and a supporting structure connected to the cell culture portion.Type: ApplicationFiled: December 19, 2020Publication date: June 24, 2021Inventors: Aaron DELAHANTY, Dries BRAEKEN, Alexandru ANDREI, Peter PEUMANS, Carolina MORA LOPEZ, Veerle REUMERS, Veronique ROCHUS, Bart WEEKERS
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Publication number: 20210191316Abstract: A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity termType: ApplicationFiled: December 19, 2020Publication date: June 24, 2021Inventors: Zhenxiang LUO, Abdulkadir YURT, Dries BRAEKEN, Liesbet LAGAE, Richard STAHL
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Publication number: 20200348288Abstract: A method for analysis of cardiomyocyte function comprises: receiving (302) in a measurement position a substrate (110) carrying cardiomyocytes on a cell culturing surface; recording (304) electrical signals from the cardiomyocytes; simultaneously with said recording, acquiring (306) a sequence of images of the cardiomyocytes on the cell culturing surface based on detecting light wavefront information of reflected light; determining (308) an electrical representation relating to an intracellular and/or extracellular action potential, and/or an impedimetric electrical image of a cardiomyocyte based on the recorded electrical signals; and determining (310) a contractile representation relating to cellular deformation during contractility action of a group of cardiomyocytes based on the acquired sequence of images, wherein the electrical representation and the contractile representation apply to a common period of time.Type: ApplicationFiled: December 19, 2018Publication date: November 5, 2020Inventors: Dries BRAEKEN, Thomas PAUWELYN, Liesbet LAGAE
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Patent number: 10697882Abstract: The present disclosure relates to devices and methods configured to perform drug screening on cells. At least one embodiment relates to a lens-free device for performing drug screening on cells. The lens-free device includes a substrate having a surface. The lens-free device also includes a light source positioned to illuminate the cells, when present, on the substrate surface with a light wave. The lens-free device further includes a sensor positioned to detect an optical signal caused by illuminating the cells. The substrate surface includes a microelectrode array for sensing an electrophysiological signal from the cells.Type: GrantFiled: April 3, 2015Date of Patent: June 30, 2020Assignee: IMEC VZWInventors: Veerle Reumers, Dries Braeken, Geert Vanmeerbeeck, Richard Stahl, Andy Lambrechts
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Publication number: 20190391127Abstract: A device for analysis of cells comprises: an integrated circuit arrangement on a substrate; a dielectric layer formed above the integrated circuit arrangement; a microelectrode array layer formed above the dielectric layer, said microelectrode array layer comprising a plurality of individual electrodes, wherein each electrode is connected to the integrated circuit arrangement through a via in the dielectric layer; and a plurality of longitudinal trenches in the dielectric layer and the microelectrode array layer for stimulating cell growth on a surface of the device.Type: ApplicationFiled: June 24, 2019Publication date: December 26, 2019Inventors: Dries BRAEKEN, Veerle REUMERS, Alexandru ANDREI, Andrea FIRRINCIELI, Thomas PAUWELYN
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Patent number: 10159419Abstract: In an aspect of the disclosure, a stimulation device includes a probe attached to a first support. The probe includes at least one grating coupler for coupling light into the probe. The device further includes at least one optical source for providing an optical stimulation signal mounted on a second support, and at least one means for detachably attaching the first support to the second support. The position of the at least one optical source is aligned with the position of the at least one grating coupler to allow light emitted from the at least one optical source to be received by the at least one grating coupler.Type: GrantFiled: May 29, 2014Date of Patent: December 25, 2018Assignees: IMEC, KATHOLIEKE UNIVERSITEIT LEUVEN, KU LEUVEN R&DInventors: Luis Diego Leon Hoffman, Dries Braeken, Silke Musa
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Publication number: 20180161065Abstract: A surgical insertion device is disclosed, comprising an elongated central flat region tapering from one distal end having a device area, configured to receive an electronic device, towards the opposite distal end having a sharp tip.Type: ApplicationFiled: December 6, 2017Publication date: June 14, 2018Applicants: Imec VZW, Universiteit GentInventors: Maria Op de Beeck, Bjorn Vandecasteele, Dries Braeken
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Publication number: 20170115201Abstract: The present disclosure relates to devices and methods configured to perform drug screening on cells. At least one embodiment relates to a lens-free device for performing drug screening on cells. The lens-free device includes a substrate having a surface. The lens-free device also includes a light source positioned to illuminate the cells, when present, on the substrate surface with a light wave. The lens-free device further includes a sensor positioned to detect an optical signal caused by illuminating the cells. The substrate surface includes a microelectrode array for sensing an electrophysiological signal from the cells.Type: ApplicationFiled: April 3, 2015Publication date: April 27, 2017Applicant: IMEC VZWInventors: Veerle Reumers, Dries Braeken, Geert Vanmeerbeeck, Richard Stahl, Andy Lambrechts
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Patent number: 9551698Abstract: The electronic device for sensing and/or actuating comprises a device surface to which an biological cell (40) is applied, further comprising a sensor and/or an actuator (25), and an access channel (20) with a channel port (21), said channel port being located in said surface. The access channel (20) is designed such that the biological cell (40) can enter the access channel (20) to thereby provide access to the sensor (25). Particularly the cell (40) forms a protruding portion (41) by entering the access channel (20), which portion is sensed. The access channel (20) may be provided with a specific sensor port at which the sensor (25) is present. The device may be used for sensing or actuating biological cells, such as neurons, for instance in electroporation treatments.Type: GrantFiled: December 30, 2010Date of Patent: January 24, 2017Assignees: IMEC, Katholieke Universiteit Leuven, KU Leuven R&DInventors: Roeland Huys, Wolfgang Eberle, Dries Braeken, Liesbeth Micholt
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Publication number: 20140356892Abstract: In an aspect of the disclosure, a stimulation device includes a probe attached to a first support. The probe includes at least one grating coupler for coupling light into the probe. The device further includes at least one optical source for providing an optical stimulation signal mounted on a second support, and at least one means for detachably attaching the first support to the second support. The position of the at least one optical source is aligned with the position of the at least one grating coupler to allow light emitted from the at least one optical source to be received by the at least one grating coupler.Type: ApplicationFiled: May 29, 2014Publication date: December 4, 2014Applicants: IMEC, KATHOLIEKE UNIVERSITEIT LEUVEN, KU LEUVEN R&DInventors: Luis Diego Leon Hoffman, Dries Braeken, Silke Musa
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Patent number: 8569006Abstract: The invention relates to a sensor comprising a sensing layer and a surface layer, wherein said surface layer comprises, a first region suitable for adherent growth of cells, and a second region, adjacent to said second layer, suitable for the attachment of proteins, wherein the first and second region are in contact with the sensing layer.Type: GrantFiled: February 29, 2008Date of Patent: October 29, 2013Assignees: IMEC, Katholieke Universiteit Leuven, K.U. Leuven R&DInventors: Dries Braeken, Danielle Rand, Carmen Bartic
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Publication number: 20130045528Abstract: The electronic device for sensing and/or actuating comprises a device surface to which an biological cell (40) is applied, further comprising a sensor and/or an actuator (25), and an access channel (20) with a channel port (21), said channel port being located in said surface. The access channel (20) is designed such that the biological cell (40) can enter the access channel (20) to thereby provide access to the sensor (25). Particularly the cell (40) forms a protruding portion (41) by entering the access channel (20), which portion is sensed. The access channel (20) may be provided with a specific sensor port at which the sensor (25) is present. The device may be used for sensing or actuating biological cells, such as neurons, for instance in electroporation treatments.Type: ApplicationFiled: December 30, 2010Publication date: February 21, 2013Applicants: KATHOLIEKE UNIVERSITEIT LEUVEN, K.U. LEUVEN R&D, IMECInventors: Roeland Huys, Wolfgang Eberle, Dries Braeken, Liesbeth Micholt
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Publication number: 20110269172Abstract: A mixed micro-fluidic multi-electrode grid array (MEGA) device (10) suitable for holding a tissue slice (6) and for recording and/or stimulating neuron cells from said tissue slice (6), the MEGA device (10) comprising at least a top substrate in the form of a grid (4) comprising at least an electrical and/or optical multi-electrode array and a bottom substrate in the form of a stack made of a grid (1) comprising an electrical and/or optical multi-electrode array and a backbone (2) underneath said grid (1) comprising a micro-fluidic perfusion system. Furthermore said MEGA device (10) comprises means (5) for pressing and positioning said first and second substrate together and adhering a tissue slice in between said two substrates.Type: ApplicationFiled: April 27, 2011Publication date: November 3, 2011Applicants: KATHOLIEKE UNIVERSITEIT LEUVEN, K.U. LEUVEN R&D, IMECInventors: Wolfgang Eberle, Dries Braeken, Roeland Huys, Dimiter Prodanov
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Publication number: 20110257501Abstract: A bio-hybrid implant suitable for recording and/or stimulating cells, the implant comprising (a) at least one closed insulated chamber (1) containing a substrate (502) with a neural interface (13) for connecting neurons to an electronic circuit, (b) at least one flexible guiding channel (10) having a first interface (11) to connect to at least one of the closed insulated chambers (1) and a second interface (9) to connect to a hosts' nerve system (7) or to another insulated chamber.Type: ApplicationFiled: April 18, 2011Publication date: October 20, 2011Applicants: KATHOLIEKE UNIVERSITEIT LEUVEN, K.U. LEUVEN R&D, IMECInventors: Roeland Huys, Dries Braeken, Dimiter Prodanov, Wolfgang Eberle, Kris Verstreken
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Publication number: 20110003322Abstract: The invention relates to a sensor (1) comprising a sensing layer (2) and a surface layer (3), wherein said surface layer comprises, a first region (4) suitable for adherent growth of cells (6), and a second region (5), adjacent to said second layer, suitable for the attachment of proteins, wherein the first and second region are in contact with the sensing layer.Type: ApplicationFiled: February 29, 2008Publication date: January 6, 2011Inventor: Dries BRAEKEN