Patents by Inventor Duane Inlow
Duane Inlow has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7851433Abstract: Highly purified preparations of TFPI or TFPI analogs can be prepared using a method that generally involves the following steps: (1) expression of TFPI or TFPI analog in E. coli, (2) isolation of refractile bodies, (3) dissolution of the refractile bodies and refolding of the expressed TFPI or TFPI analog, (4) SP-Sepharose fast flow (FF) chromatography, (5) a first concentration and diafiltration step, (6) Q-Sepharose high (HP) performance chromatography, (7) butyl hydrophobic interaction chromatography (HIC), (8) SP-Sepharose HP chromatography, and (9) a second concentration/diafiltration step. Less than about 12% of the TFPI or TFPI analog molecules in such preparations are modified TFPI or TFPI analog species (i.e., oxidized, carbamylated, acetylated, deamidated, aggregated, or misfolded species).Type: GrantFiled: January 8, 2004Date of Patent: December 14, 2010Assignees: Novartis Vaccines and Diagnostics, Inc., Pharmacia & Upjohn CompanyInventors: David H. Reifsnyder, Duane Inlow, Glenn Dorin, Patricio T. Riquelme, Cynthia A. Cowgill, Douglas G. Bolesch, Mark E. Gustafson
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Patent number: 7070960Abstract: A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One- to three-fold increases in antibody yield have been obtained by these methods.Type: GrantFiled: May 9, 2005Date of Patent: July 4, 2006Assignee: Chiron CorporationInventors: Brian Maiorella, Duane Inlow, William Howarth
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Publication number: 20050221393Abstract: A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One- to three-fold increases in antibody yield have been obtained by these methods.Type: ApplicationFiled: May 9, 2005Publication date: October 6, 2005Applicant: Chiron CorporationInventors: Brian Maiorella, Duane Inlow, William Howarth
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Patent number: 6905847Abstract: A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One-to three-fold increases in antibody yield have been obtained by these methods.Type: GrantFiled: May 30, 2001Date of Patent: June 14, 2005Assignee: Chiron CorporationInventors: Brian Maiorella, Duane Inlow, William Howarth
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Publication number: 20050037475Abstract: Highly purified preparations of TFPI or TFPI analogs can be prepared using a method that generally involves the following steps: (1) expression of TFPI or TFPI analog in E. coli, (2) isolation of refractile bodies, (3) dissolution of the refractile bodies and refolding of the expressed TFPI or TFPI analog, (4) SP-Sepharose fast flow (FF) chromatography, (5) a first concentration and diafiltration step, (6) Q-Sepharose high (HP) performance chromatography, (7) butyl hydrophobic interaction chromatography (HIC), (8) SP-Sepharose HP chromatography, and (9) a second concentration/diafiltration step. Less than about 12% of the TFPI or TFPI analog molecules in such preparations are modified TFPI or TFPI analog species (i.e., oxidized, carbamylated, acetylated, deamidated, aggregated, or misfolded species).Type: ApplicationFiled: January 8, 2004Publication date: February 17, 2005Applicants: Chiron Corporation, Pharmacia & Upjohn CompanyInventors: David Reifsnyder, Duane Inlow, Glenn Dorin, Patricio Riquelme, Cynthia Cowgill, Douglas Bolesch, Mark Gustafson
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Publication number: 20020119530Abstract: A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One- to three-fold increases in antibody yield have been obtained by these methods.Type: ApplicationFiled: May 30, 2001Publication date: August 29, 2002Inventors: Brian Maiorella, Duane Inlow, William Howarth
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Patent number: 6238891Abstract: A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One- to three-fold increases in antibody yield have been obtained by these methods.Type: GrantFiled: June 7, 1995Date of Patent: May 29, 2001Assignee: Cetus Oncology CorporationInventors: Brian Maiorella, Duane Inlow, William Howarth
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Patent number: 6048728Abstract: Protein-free cell culture media supplements are described consisting of synergistic combinations of medium components, which when added to cell culture media, either serum supplemented or serum-free, enhance cell growth, culture longevity and product expression.Type: GrantFiled: June 7, 1995Date of Patent: April 11, 2000Assignee: Chiron CorporationInventors: Duane Inlow, Brian Maiorella, William Howarth
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Patent number: 5372943Abstract: Lipid microemulsions which can be added to cell culture media to provide essential lipids in a bioavailable form and their components are disclosed. Methods to disperse lipids in culture media by the use of one or more emulsifiers are described. Further disclosed are media which support growth of cells and production of recombinant, viral and/or native products wherein lipids are supplied in the form of microemulsion.Type: GrantFiled: July 12, 1993Date of Patent: December 13, 1994Assignee: Cetus CorporationInventors: Duane Inlow, Brian Maiorella
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Patent number: 5156964Abstract: This invention is in the field of cell and/or tissue culture. In particular, this invention relates to methods which adapt cells to a desired phenotype by exposing the cells to high levels of ammonia in culture, and subsequently transferring the adapted cells to a new culture medium in which there is no initial level of ammonia or the initial level of ammonia is below the level to which cells have been exposed to during the adaptation process. In this new culture medium, the adapted cells express the desired phenotype of growing to a higher viable cell density, and/or remaining viable for a longer period of time, and/or producing more of a desired cell product than their non-adapted counterparts grown in the same medium. This invention includes the adapted cells produced thereby and their cell products.Type: GrantFiled: January 21, 1992Date of Patent: October 20, 1992Assignee: Cetus CorporationInventors: Duane Inlow, Brian Maiorella, Andrea E. Shauger
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Patent number: 5024947Abstract: Serum-free media which support the growth of insect cells and the production thereby of recombinant proteins and viral products are herein disclosed. The serum free media can support insect cell growth at large scale under agitated and/or sparged, preferably well-aerated conditions.The serum free medium disclosed support insect cell growth to cell densities comparable to serum-containing culture and the production of viral and recombinant products to levels equivalent to those found in serum containing culture.Type: GrantFiled: July 24, 1987Date of Patent: June 18, 1991Assignee: Cetus CorporationInventors: Duane Inlow, Brian Maiorella