Abstract: Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.

Abstract: Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.

Abstract: Disclosed herein, a power supply may be recharged with a generally used system. In various embodiments, cleaning systems may be used to affect a generator to cause the generator to charge a power supply. Such charging systems may be used without the need for separate or specialized power charging systems. A charging source or system may, therefore, also charge a power supply without requiring additional steps.

Abstract: The disclosure provides methods for amplification of paired transcript sequences from a single cell, such as, but not limited to alpha and beta T cell receptor (TCR) sequences from a single T cell. The method can include: placing a single T cell into a cell lysis solution to provide a T cell lysate comprising RNA; generating first strand cDNA from the RNA; amplifying the first strand cDNA to provide amplified cDNA; and amplifying the alpha and beta TCR sequences from the amplified cDNA in a single reaction, wherein the alpha and beta TCR sequences are amplified using three sets of TCR amplification primers. In some instances, the methods can be performed using no more than six sets of primers.

Abstract: A combustor dome may be formed by way of additive layer manufacturing. The combustor dome may further include a raised outer surface and a recessed outer surface on a hot side of the combustor dome. The recessed outer surfaces may be closer to the cold side than the raised outer surfaces. The combustor dome may include a shadow surface defined between the raised outer surface and recessed outer surface. The shadow surface may define a corresponding cooling outlet in fluid communication with an internal cooling channel defined inside of the combustor dome. The cooling outlet may release air from the internal cooling channel to the hot axial side of the combustor dome.

Abstract: Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

Abstract: Processes and kits are provided for producing sequence specific fragments of nucleic acid molecules, whether from a genome or transcriptome, where one end of the molecule is highly diverse and/or the full-length molecule, whether a gene or a mRNA, is too long for it to be sequenced using currently available sequencing methods. Methods of preparing a sequencing library configured for 5? or 3? anchored sequencing, wherein the opposing termini of the library molecules are differentially truncated, and methods of parallel sequencing such libraries are described.

Abstract: This disclosure relates to the production and use of an isolated, purified and/or recombinant T cell receptor (TCR) that specifically binds to a mutant IDH1 protein, or a fragment thereof, wherein the mutant IDH1 protein or fragment thereof comprises an R132H mutation.

Abstract: Disclosed herein, a power supply may be recharged with a generally used system. In various embodiments, cleaning systems may be used to affect a generator to cause the generator to charge a power supply. Such charging systems may be used without the need for separate or specialized power charging systems. A charging source or system may, therefore, also charge a power supply without requiring additional steps.

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.

Abstract: Disclosed herein, a power supply may be recharged with a generally used system. In various embodiments, cleaning systems may be used to affect a generator to cause the generator to charge a power supply. Such charging systems may be used without the need for separate or specialized power charging systems. A charging source or system may, therefore, also charge a power supply without requiring additional steps.

Abstract: Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.

Abstract: A combustor dome may be formed by way of additive layer manufacturing. The combustor dome may further include a raised outer surface and a recessed outer surface on a hot side of the combustor dome. The recessed outer surfaces may be closer to the cold side than the raised outer surfaces. The combustor dome may include a shadow surface defined between the raised outer surface and recessed outer surface. The shadow surface may define a corresponding cooling outlet in fluid communication with an internal cooling channel defined inside of the combustor dome. The cooling outlet may release air from the internal cooling channel to the hot axial side of the combustor dome.

Abstract: Disclosed herein, a power supply may be recharged with a generally used system. In various embodiments, cleaning systems may be used to affect a generator to cause the generator to charge a power supply. Such charging systems may be used without the need for separate or specialized power charging systems. A charging source or system may, therefore, also charge a power supply without requiring additional steps.

Abstract: A free space optical communication system (100) and method including a constellation of several satellites (102). Each of satellites including: several inter-satellite optical telescopes (204) for optical communication with multiple neighboring satellites, each inter-satellite optical telescope is capable of adjusting its elevation angle to accommodate changes in the number of satellites in the constellation; and several up/down link optical telescopes (206) for optical communication with multiple ground sites, where each ground site has several ground optical telescopes. As the constellation passes a given ground site, some of the up/down-link telescopes of a given satellite are configured to track at least two respective ground optical telescopes of the given ground site and send data to the ground optical telescope with the clearest line of sight to the given satellite.

Abstract: A free space optical communication system (100) and method including a constellation of several satellites (102). Each of satellites including: several inter-satellite optical telescopes (204) for optical communication with multiple neighboring satellites, each inter-satellite optical telescope is capable of adjusting its elevation angle to accommodate changes in the number of satellites in the constellation; and several up/down link optical telescopes (206) for optical communication with multiple ground sites, where each ground site has several ground optical telescopes. As the constellation passes a given ground site, some of the up/down-link telescopes of a given satellite are configured to track at least two respective ground optical telescopes of the given ground site and send data to the ground optical telescope with the clearest line of sight to the given satellite.

Abstract: A free space optical communication system (100) and method including: several optical beam expanders (414) for receiving incoming optical signals from ground sites and neighboring satellites; several optical preamplifiers (412) for preamplifying the received optical signals; one or more optical main amplifiers (404) for amplifying the preamplified optical signals; and an optical switch (408) for directing respective amplified optical signals to respective destinations via a respective optical beam expander. The respective amplified optical signals are inputted to a respective optical beam expander (414) for transmission to said respective destinations, as outgoing optical signals.

Abstract: A free space optical communication system (100) and method including a constellation of several satellites (102). Each of satellites including: several inter-satellite optical telescopes (204) for optical communication with multiple neighboring satellites, each inter-satellite optical telescope is capable of adjusting its elevation angle; and several up/down link optical telescopes (206) for optical communication with multiple ground sites. As the constellation passes a given ground site, some of the up/down-link telescopes of a given satellite are configured to track at least two respective ground optical telescopes of the given ground site and send data to the ground optical telescope with the clearest line of sight to the given satellite. Moreover, each of the satellites includes optical circuitry (208, 210, 212, 216) for optically processing and switching incoming and outgoing optical signals without converting the optical signals into electrical signals.

Abstract: An architecture for creating a plasma-processing-related application is provided. The architecture includes a foundation layer having a plurality of framework components, wherein the framework components including at least one of security component, data management component and user interface (UI) component. The architecture also includes an analysis view layer having a set of views, wherein the set of views are generated utilizing components from the foundation layer. The architecture further includes an analysis module layer having a plurality of analysis modules, wherein each analysis module of the plurality of analysis modules is assembled from one or more view of the set of views. The architecture moreover includes an application layer having a plurality of applications, wherein each application of the plurality of applications is assembled from one or more analysis module.