Abstract: A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite below an inhibitory concentration in the cell culture medium for the at least one metabolite, wherein the at least one metabolite is aconitic acid (AA), leucinic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid (MSA), trigonelline (TRI), N-acetylputrescinium (NAP), or a combination thereof, and wherein the enzyme comprises ADH5, BCAT1, CAT, GOT1, HADHB, HOGA1, SLC35A1, or a combination thereof.

Abstract: A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite below an inhibitory concentration in the cell culture medium for the at least one metabolite, wherein the at least one metabolite is aconitic acid (AA), leucinic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid (MSA), trigonelline (TRI), N-acetylputrescinium (NAP), or a combination thereof, and wherein the enzyme comprises ADH5, BCAT1, CAT, GOT1, HADHB, HOGA1, SLC35A1, or a combination thereof.

Abstract: A printing apparatus includes: a flushing determination unit, and a storage unit, wherein the flushing determination unit is configured to: calculate a temporary return position, a temporary reciprocating movement time that is necessary when the carriage is returned at the temporary return position, and a threshold value that is a longest non-ejection time during which printing can be performed in a stable manner at an environmental temperature measured by a thermometer, based on print data to be printed in reciprocating movement of the carriage; and determine whether the temporary reciprocating movement time exceeds the threshold value, wherein when the temporary reciprocating movement time exceeds the threshold value, the flushing determination unit sets a position at which the one reciprocating movement time becomes longer than the temporary reciprocating movement time as a return position in the reciprocating movement, and determines to perform the flushing in a return path.

Abstract: A printing apparatus includes: a flushing determination unit, and a storage unit, wherein the flushing determination unit is configured to: calculate a temporary return position, a temporary reciprocating movement time that is necessary when the carriage is returned at the temporary return position, and a threshold value that is a longest non-ejection time during which printing can be performed in a stable manner at an environmental temperature measured by a thermometer, based on print data to be printed in reciprocating movement of the carriage; and determine whether the temporary reciprocating movement time exceeds the threshold value, wherein when the temporary reciprocating movement time exceeds the threshold value, the flushing determination unit sets a position at which the one reciprocating movement time becomes longer than the temporary reciprocating movement time as a return position in the reciprocating movement, and determines to perform the flushing in a return path.

Abstract: A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite below an inhibitory concentration in the cell culture medium for the at least one metabolite, wherein the at least one metabolite is aconitic acid (AA), leucinic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid (MSA), trigonelline (TRI), N-acetylputrescinium (NAP), or a combination thereof, and wherein the enzyme comprises ADH5, BCAT1, CAT, GOT1, HADHB, HOGA1, SLC35A1, or a combination thereof.