Abstract: A brush assembly and method of using such brush assembly for obtaining endocervical tissue samples. The brush assembly includes a handle having an outer surface with a plurality of pointed edges and grooved portions disposed in an alternating manner about the outer surface and extending the length of the handle to facilitate manipulation and rotation of the handle during use without slippage. A brush is attached to one end of the handle for collecting tissue. A semi-hemispherical bead is attached to the end of the brush to prevent patient discomfort during insertion of the brush assembly into the vagina. A sleeve surrounding the brush assembly protects the brush from contamination by vaginal tissue during insertion and removal of the brush assembly. During insertion, the sleeve covers the brush. When the brush is in its desired sampling position, the sleeve is slid away from the brush to expose the brush.