Abstract: A multi-functional centrifuge bucket designed for use with a cell processing cassette (CPC) in automated cell processing platforms. The centrifuge bucket features a secure mounting interface for precise CPC alignment, an integrated temperature control system to maintain stable conditions, and a pivoting mechanism for tilting or rotation to enable uniform mixing. Real-time monitoring of cell sedimentation and distribution is facilitated by optical sensors, while a data communication system supports automated adjustments to centrifugation and mixing parameters. The heating mechanism includes thermal insulation layers and advanced fail-safe controls, ensuring precise temperature regulation within the CPC while preventing external overheating. Methods of use include spinoculation, sedimentation, and precise fluid mixing, enabled by controlled motion and feedback systems.

Abstract: A functionally closed cell processing platform designed to minimize contamination and automate cell processing. The platform integrates multiple specialized cassettes, including a reagent sample cassette (RSC) for reagent delivery and sample collection, a process fluids cassette (PFC) for waste and buffer management, and a cell processing cassette (CPC) for cell handling, along with a transfer syringe cassette (TSC) for precise fluid transfer. The system features a centrifuge capable of multi-mode operation for cell concentration and mixing, pneumatic controls for fluid and microbubble manipulation, and UV sanitizers to maintain aseptic conditions. Advanced functionalities include optical and thermal sensors for real-time monitoring, automated control of fluid and reagent handling, and a microbubble cell selection system for precise target cell isolation.

Abstract: The invention relates to a method and system for maintaining aseptic conditions and verifying filter integrity in a cell processing platform. The platform features an integrated pressure decay testing module to evaluate the functionality of hydrophilic and hydrophobic filters before and after processing. The system operates by pressurizing filters, measuring pressure decay, and determining integrity based on predefined thresholds, with results logged for compliance. The platform includes a cell processing cassette (CPC) equipped for sequential cell processing steps, such as washing, selection, activation, and harvesting, within a single chamber. Automated fluid and gas transfer systems, incorporating filters and emergency recovery pathways, ensure contamination-free operations. Additional features include UV disinfection, adaptive control systems for parameter optimization, and temperature-controlled environments to support cell viability.

Abstract: A method for enhancing genetic modification of target cells through automated spinoculation within a functionally closed cell processing platform. The process begins with introducing a cell suspension into a tapered cell processing cassette (CPC) that facilitates sedimentation and concentration of target cells. Genetic modification is achieved by injecting a gene transfer vector into the CPC, followed by controlled spinoculation to enhance vector-cell interaction through centrifugal force while maintaining optimal temperature and conditions. Optical sensors and automated feedback systems monitor and adjust centrifugation parameters to maximize transduction efficiency and cell viability. The method includes precise mixing, reagent delivery, washing to remove contaminants, and harvesting of genetically modified cells, all within a sterile environment.

Abstract: An automatic balancing system for a centrifuge designed to maintain stability and minimize vibration during rotation. The system incorporates a central rotor with radially aligned linear actuators to adaptively counterbalance variations in fluid volume and weight distribution within cell processing cassettes (CPCs). It features accelerometers and position sensors for real-time monitoring and adjustment, with a user interface enabling automated or manual optimization of counterbalance weights. Additional innovations include radar-based fluid-level sensing for precise weight estimations, advanced optical detection systems for monitoring cell sedimentation, and a centrifuge control module to adjust rotational speed based on cell density feedback. The system also includes a temperature regulation mechanism, locking actuators, and multi-spectral imaging capabilities for enhanced processing accuracy.

Abstract: There are many inventions described and illustrated herein. In one aspect, the present invention is directed to a portable fuel cell power and management system (for example, hydrogen and/or methanol based systems), components and/or elements thereof, as well as techniques for controlling and/or operating such systems. The fuel cell power management system (and method of controlling and/or operating same) actively monitors, manages and/or controls one or more operating parameter(s) of the fuel cell system. For example, the system monitors, manages and/or controls the consumption and/or the rate of consumption of fuel by the system, and in response thereto, may provide and/or alert the user to amount of fuel remaining, consumed, the rate of consumption and/or the time (or estimation thereof) remaining until all of the fuel is spent. In this way, the user may schedule or plan accordingly.

Abstract: There are many inventions described and illustrated herein. In one aspect, the present invention is directed to a portable fuel cell power and management system (for example, hydrogen and/or methanol based systems), components and/or elements thereof, as well as techniques for controlling and/or operating such systems. The fuel cell power management system (and method of controlling and/or operating same) actively monitors, manages and/or controls one or more operating parameter(s) of the fuel cell system. For example, the system monitors, manages and/or controls the consumption and/or the rate of consumption of fuel by the system, and in response thereto, may provide and/or alert the user to amount of fuel remaining, consumed, the rate of consumption and/or the time (or estimation thereof) remaining until all of the fuel is spent. In this way, the user may schedule or plan accordingly.

Abstract: There are many inventions described and illustrated herein. In one aspect, the present invention is directed to a portable fuel cell power and management system (for example, hydrogen and/or methanol based systems), components and/or elements thereof, as well as techniques for controlling and/or operating such systems. The fuel cell power management system (and method of controlling and/or operating same) actively monitors, manages and/or controls one or more operating parameter(s) of the fuel cell system. For example, the system monitors, manages and/or controls the consumption and/or the rate of consumption of fuel by the system, and in response thereto, may provide and/or alert the user to amount of fuel remaining, consumed, the rate of consumption and/or the time (or estimation thereof) remaining until all of the fuel is spent. In this way, the user may schedule or plan accordingly.

Abstract: There are many inventions described and illustrated herein. In one aspect, the present invention is directed to a portable fuel cell power and management system (for example, hydrogen and/or methanol based systems), components and/or elements thereof, as well as techniques for controlling and/or operating such systems. The fuel cell power management system (and method of controlling and/or operating same) actively monitors, manages and/or controls one or more operating parameter(s) of the fuel cell system. For example, the system monitors, manages and/or controls the consumption and/or the rate of consumption of fuel by the system, and in response thereto, may provide and/or alert the user to amount of fuel remaining, consumed, the rate of consumption and/or the time (or estimation thereof) remaining until all of the fuel is spent. In this way, the user may schedule or plan accordingly.

Abstract: There are many inventions described and illustrated herein. In one aspect, the present invention is directed to a portable fuel cell power and management system (for example, hydrogen and/or methanol based systems), components and/or elements thereof, as well as techniques for controlling and/or operating such systems. The fuel cell power management system (and method of controlling and/or operating same) actively monitors, manages and/or controls one or more operating parameter(s) of the fuel cell system. For example, the system monitors, manages and/or controls the consumption and/or the rate of consumption of fuel by the system, and in response thereto, may provide and/or alert the user to amount of fuel remaining, consumed, the rate of consumption and/or the time (or estimation thereof) remaining until all of the fuel is spent. In this way, the user may schedule or plan accordingly.

Abstract: There are many inventions described and illustrated herein. In one aspect, the present invention is directed to a portable fuel cell power and management system (for example, hydrogen and/or methanol based systems), components and/or elements thereof, as well as techniques for controlling and/or operating such systems. The fuel cell power management system (and method of controlling and/or operating same) actively monitors, manages and/or controls one or more operating parameter(s) of the fuel cell system. For example, the system monitors, manages and/or controls the consumption and/or the rate of consumption of fuel by the system, and in response thereto, may provide and/or alert the user to amount of fuel remaining, consumed, the rate of consumption and/or the time (or estimation thereof remaining until all of the fuel is spent. In this way, the user may schedule or plan accordingly.

Abstract: There are many inventions described and illustrated herein. In one aspect, the present invention is directed to a portable fuel cell power and management system (for example, hydrogen and/or methanol based systems), components and/or elements thereof, as well as techniques for controlling and/or operating such systems. The fuel cell power management system (and method of controlling and/or operating same) actively monitors, manages and/or controls one or more operating parameter(s) of the fuel cell system. For example, the system monitors, manages and/or controls the consumption and/or the rate of consumption of fuel by the system, and in response thereto, may provide and/or alert the user to amount of fuel remaining, consumed, the rate of consumption and/or the time (or estimation thereof remaining until all of the fuel is spent. In this way, the user may schedule or plan accordingly.

Abstract: An electronic assembly that incorporates a heat sink. The subassembly includes an integrated circuit package that is mounted to a substrate. The substrate is mounted to a spacer block which includes a pin field that contains a plurality of pins. The heat sink is coupled to the integrated circuit package by a clip that wraps around the sink and is attached to the spacer block.

Abstract: The present invention relates to electronic packaging for PC Cards, and more particularly to attaching the PC Card covers to the printed circuit board and frame. The purpose of the present invention is to provide a PC card assembly comprising of a printed circuit board coupled to the frame and attached to a bottom cover and a top cover by tabs in the bottom cover. The present invention also provides a method of assembling the PC Cards such that the tabs in the bottom cover are inserted into the through openings in the frame. These inserted tabs are then bonded to the top cover, creating a very rigid assembly and providing a very fast manufacturing process throughput.