Abstract: It is provided a method for evaluating damage of an organ tissue, in particular ischemic damage/injury of an organ tissue. The method includes the steps of measuring the concentration of at least one marker molecule in the perfusate of the organ tissue, where the measured concentration of the at least one marker molecule in the perfusate is used in at least one computer based prediction algorithm for generating at least one success score. The success score has been previously defined based on at least one parameter value of at least one pre-defined parameter. The at least one parameter value is determined after a transplantation of the organ tissue; and wherein based on the at least one success score at least one signal and/or at least one set of data is generated for facilitating the decision, if the organ tissue is suitable for transplantation or not.

Abstract: A perfusion loop assembly for ex vivo liver perfusion includes a pump providing perfusion fluid through a line branching at a branching point into a first branch line and a second branch line. The first branch line provides a first portion of the perfusion fluid to the hepatic artery of the liver, the first branch line coupled with a gas exchanger, where the first branch line includes a flow rate sensor and/or a pressure sensor. The second branch line provides a second portion of the perfusion fluid to the portal vein of the liver; the second branch line includes a valve for controlling flow of perfusion fluid into the portal vein. The second branch line includes a flow rate sensor and/or a pressure sensor. A liver chamber assembly holds the liver ex vivo, and an outlet line for the perfusion fluid connects the liver chamber assembly and the pump.