Abstract: A dental floss holding assembly comprising a pair of holders, one each for being held in a hand while dental floss suspended under tension therebetween is manipulated in dental interstices. Each of the pair of holders comprises a compact, hand-holdable member for being held in the palm of the hand, the member having positioned thereon an outwardly-extending disk defining a floss-retaining groove between the member and the disk for securing one wrapped end of the length of floss therearound. In another embodiment, a box holding a supply of floss is provided with a disk around which the floss is wrapped to hold in one hand.