Patents by Inventor Ece Alpaslan

Ece Alpaslan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions and methods for detecting pyrophosphate products of enzyme reactions using pyridylazoaniline dyes
    Patent number: 11512342
    Abstract: Provided herein is a composition comprising an enzyme that releases pyrophosphate from a substrate and a dye of Formula 1. A method for detecting pyrophosphate is also provided. A kit comprising a polymerase that releases pyrophosphate by hydrolysis of nucleoside triphosphates during nucleic acid replication, a divalent manganese salt, and the dye are also provided. The present composition, method and kits provide a way to detect and/or quantify substrates or products of enzyme reacted substrates associated with the release pyrophosphate (e.g., nucleic acid amplification reactions and other reactions that hydrolyze ATP) via a distinct color change without substantially affecting the sensitivity and/or specificity of the reaction.
    Type: Grant
    Filed: May 4, 2022
    Date of Patent: November 29, 2022
    Assignee: New England Biolabs, Inc.
    Inventors: Nathan Tanner, Ivan R. Correa, Jr., Yinhua Zhang, Ece Alpaslan