Abstract: The present invention describes a method for treating a biological material, said method comprising exposing the biological material to vacuum impregnation or pressure impregnation, preferably vacuum impregnation, in an aqueous impregnation solution comprising at least one surfactant.

Abstract: The present invention describes a method for treating a biological object being a cutting, root, sprout, budwood, root-stock, forest plant, fruit, vegetable, green leave, bulb, seed, or berry, said method comprising — arranging the biological object in an aqueous impregnation solution so that at least a portion of the biological object is immersed in the impregnation solution, but where at least another part of the biological object is kept free from aqueous impregnation solution; and — applying vacuum impregnation or pressure impregnation, preferably vacuum impregnation, to the aqueous impregnation solution when said at least portion of the biological object is immersed into the aqueous impregnation solution.

Abstract: The present invention describes a method for treating a plant material, said method comprising the following steps: —exposing the plant material to vacuum impregnation in an aqueous solution; —applying a pulsed electrical field (PEF) treatment to the plant material in the aqueous solution before, simultaneously as or after exposing the plant material to vacuum impregnation in an aqueous solution; —applying a drying step to the PEF treated plant material for removing water/moisture from surfaces of the plant material before packing the treated plant material; said method also comprising an active step for preventing microbial contamination of the aqueous solution.

Abstract: A method for extending shelf life of a biological soft tissue of an object includes introducing one or more cell protecting agents into the extracellular and intracellular space of the biological soft tissue; by impregnating the biological soft tissue by applying a vacuum to the biological soft tissue and then releasing the vacuum, wherein the step of applying a vacuum is performed while the biological soft tissue is immersed in an impregnation solution containing said one or more cell protecting agents, and by gradually decreasing the pressure from atmospheric pressure to a minimum pressure in a pressure falling step during a time of from 1 second to 15 minutes, then optionally keeping the minimum pressure in a pressure holding step during a certain time, and then rising the pressure to atmospheric pressure again in a pressure rising step during a time of from 1 second to 15 minutes.