Abstract: The invention provides isolated pyruvate dehydrogenase kinase nucleic acids and their encoded polypeptides. The present invention provides methods and compositions relating to altering pyruvate dehydrogenase kinase levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

Abstract: This invention relates to an isolated nucleic acid fragment encoding an acyltransferase, more specifically a phospholipid:diacylglycerol acyltransferase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the phospholipid:diacylglycerol acyltransferase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the phospholipid:diacylglycerol acyltransferase in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding an acyltransferase, more specifically a phospholipid:diacylglycerol acyltransferase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the phospholipid:diacylglycerol acyltransferase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the phospholipid:diacylglycerol acyltransferase in a transformed host cell.

Abstract: The preparation and use of nucleic acid fragments encoding plant fatty acid modifying enzymes associated with modification of the delta-9 position of fatty acids, in particular, formation of conjugated double bonds are disclosed. Chimeric genes incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. The preparation and use of nucleic acid fragments encoding plant fatty acid modifying enzymes associated with formation of a trans delta-12 double bond also are disclosed. Chimeric genes incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles.

Abstract: This invention relates to isolated polynucleotides encoding chlorophyllases. The invention also relates to the construction of recombinant DNA fragments comprising all or a portion of the isolated chlorophyllase-encoding polynucleotides, in sense or antisense orientation, wherein expression of the recombinant DNA fragment results in production of altered levels of chlorophyllase in a transformed host cell.

Abstract: The invention provides isolated nucleic acids and their encoded polypeptides that alter tocol content in seeds. The invention further provides expression cassettes, host cells and transformed plants containing the nucleic acids. The present invention further provides methods for altering tocol content in seeds.

Abstract: This invention relates to an isolated nucleic acid fragment encoding an acyltransferase, more specifically a phospholipid:diacylglycerol acyltransferase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the phospholipid:diacylglycerol acyltransferase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the phospholipid:diacylglycerol acyltransferase in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a gene involved in petroselinic acid biosynthesis. The invention also relates to the construction of a chimeric gene encoding all or a portion of the gene involved in petroselinic acid biosynthesis, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the gene involved in petroselinic acid biosynthesis in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a delta-6 desaturase or sphingolipid desaturase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the delta-6 desaturase or sphingolipid desaturase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the delta-6 desaturase or sphingolipid desaturase in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding an enzyme involved in lipid biosynthesis. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme involved in lipid biosynthesis, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme involved in lipid biosynthesis in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a triacylglycerol lipase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the triacylglycerol lipase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the triacylglycerol lipase in a transformed host cell.

Abstract: An isolated nucleic acid fragment encoding a cytochrome P450 enzyme associated with the synthesis of plant &Dgr;12-epoxy fatty acids is disclosed. The invention also relates to making a chimeric construct encoding all or a portion of the cytochrome P450 enzymes associated with the synthesis of plant &Dgr;12-epoxy fatty acids, in sense or antisense orientation, wherein expression of the chimeric construct results in production of altered levels of the cytochrome P450 enzymes associated with the synthesis of plant &Dgr;12-epoxy fatty acids in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a diacylglycerol acyltransferase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the diacylglycerol acyltransferase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the diacylglycerol acyltransferase in a transformed host cell.

Abstract: The invention provides isolated nucleic acids and their encoded polypeptides that alter tocol content in seeds. The invention further provides expression cassettes, host cells and transformed plants containing the nucleic acids. The present invention further provides methods for altering tocol content in seeds.

Abstract: The present invention relates to a method for producing mutants of a fatty acid desaturase having a substantially increased activity towards fatty acid substrates with chains containing fewer than 18 carbons relative to an unmutagenized precursor desaturase having an 18 carbon atom chain length substrate specificity. The method involves inducing one or more mutations in the nucleic acid sequence encoding the precursor desaturase, transforming the mutated sequence into an unsaturated fatty acid auxotroph cell such as MH13 E. coli, culturing the cells in the absence of supplemental unsaturated fatty acids, thereby selecting for recipient cells which have received and which express a mutant fatty acid desaturase with an elevated specificity for fatty acid substrates having chain lengths of less than 18 carbon atoms. A variety of mutants having 16 or fewer carbon atom chain length substrate specificities are produced by this method.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a lipase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the lipase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the lipase in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding an SSE1 protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the SSE1 protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the SSE1 protein in a transformed host cell. The present invention also relates to methods using the SSE1 protein in modulating formation of storage organelles and storage compounds in seeds, and in discovering compounds with potential herbicidal activity.

Abstract: This invention relates to isolated nucleic acid fragments encoding lipoxygenases. The invention also relates to the construction of recombinant DNA constructs encoding all or a portion of the lipoxygenase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the lipoxygenase in a transformed host cell.

Abstract: An isolated nucleic acid fragment encoding a cytochrome P450 enzyme associated with the synthesis of plant &Dgr;12-epoxy fatty acids is disclosed. The invention also relates to making a chimeric construct encoding all or a portion of the cytochrome P450 enzymes associated with the synthesis of plant &Dgr;12-epoxy fatty acids, in sense or antisense orientation, wherein expression of the chimeric construct results in production of altered levels of the cytochrome P450 enzymes associated with the synthesis of plant &Dgr;12-epoxy fatty acids in a transformed host cell.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a proteinase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the proteinase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the proteinase in a transformed host cell.