Abstract: A method of forming a configurable substrate includes depositing a volume of trans-cyclooctene on a configurable substrate of a fluidic device, and forming a bioorthogonal tethered protein on the configurable substrate. The bioorthogonal tethered protein may be formed by attaching a tetrazine-modified protein or tetrazine-modified functional protein fragment to the trans-cyclooctene, wherein the tetrazine-modified protein or tetrazine-modified functional protein fragment is configured to bind to a target analyte.

Abstract: Examples are directed to rapid detection test (RDT) devices, apparatuses, and methods of forming RDT devices and/or apparatuses that include detection particles preloaded in a test region. An example RDT apparatus includes a substrate, and a test region disposed on a first portion of the substrate. The test region including: a first set of detection particles that exhibit a first detectable label, and a set of capture agents configured to bind to a target analyte in a biological sample, each of the set of capture agents including a first ligand configured to bind to the target analyte. The RDT apparatus further includes a second set of detection particles that exhibit a second detectable label, wherein each of the second set of detection particles includes a label protein including a second ligand configured to bind to the target analyte.