Abstract: Disclosed are reel-to-reel coating systems that can be used to form graphene-coated tapes. The coating systems can be modular, self-contained, and sized to allow for benchtop fabrication of coated tape substrates for use in imaging applications. The reels of the coating system can be removable, and adapted to be re-attached to an in-situ scanning electron microscope reel-to-reel imaging system, a reel-to-reel system for sectioning resin-embedded cells and/or tissues with an ultramicrotome, or any combination thereof. In this way, the coater can serve as part of an integrated system facilitating efficient, serial sample processing and imaging.

Abstract: The disclosure describes assemblies and systems for use in reel-to-reel imaging of ultrathin samples. The assemblies and the systems disclosed herein are adapted for use with a plurality of detectors and are configured for use in a variety of electron microscopes. Also, methods of using such assemblies and systems are disclosed.

Abstract: A plurality of accessory components for use in respective reel to reel sectioning, serial sectioning, and array tomography sectioning described herein incorporate mechanical and electro-mechanical engineering to provide accessory tools for attachment to and use with a microtome, as shown in several of the figures of this disclosure.

Abstract: Disclosed are articles comprising substrate and graphene coating that are configured to support a sample for electron or optical microscopy. Also disclosed are methods of making the same and methods of using the same in imaging technology.

Abstract: Disclosed are compositions and methods for the conductive fixation of organic material, including biological samples. The compositions and methods described herein can address the problems of charging and sample damage caused by electron beam-sample interactions within an electron microscope.