Abstract: Automated analysis of particles in liquid samples using a laser-scanning confocal microscope. More specifically, embodiments described herein enable a novel method and apparatus for focusing a laser-scanning microscope using the reflection of the laser beam off of the vessel containing the liquid sample.

Abstract: A waste disposal docking system for a vehicle includes a septic tank hose to attach to and extend from a septic tank hose connection fitting of the vehicle; a coiled metal extension device secured to a housing attached to the vehicle and engaged with an end of the septic tank hose; and a control system having a motor engaged with the coiled metal extension device to extend and contract the coiled metal extension device; the control system with the motor extend and contract the septic tank hose to and from a sewer cleanout station.

Abstract: A method of assaying biological content of a sample, termed “microsphere-based binding assay”, using microspheres made of polystyrene or other materials to capture and detect in a sample one or more biomarkers that may be present in the biological sample. The method is able to measure various antibodies associated with infection or vaccine response. Microspheres can be coated with capture antigens and exposed to multiple sets of biomarkers. The biomarkers can be fluorescently active or fluorescently labeled. The method analyzes the fluorescent profile using scanning cytometry. Increased safety, reliability and efficiency is achieved over prior art methods.

Abstract: A system for filtering light using a spectrometer enhanced with spectral filters using an array of independent photodetectors to measure the fluorescent or scattered light signal. A system comprising a light source, an illuminated sample, a light spectrum device, a collimator lens, a plurality of spectral filters each having a varying and selected light transmission spectrum and a plurality of photodetectors wherein each photodetector is oriented to a spectral filter. A scanning cytometer for measuring fluorescence and light scattering from an illuminated portion of the sample comprising a first light source, a scanner scanning in two axes, a fluorescence detector, an objective lens, an optically translucent medium through which a sample may be illuminated and a confocal apparatus positioned distally from the light source and sample and through which light signals from the sample are transmitted to a fluorescence detector.

Abstract: A method of making sensitive measurements of quantity of target analytes dissolved in liquid and attached to microsphere particles wherein the analyte coated particles are labeled with a fluorescent marker. The solution is placed in a light transparent container having a planar bottom and the particles allowed to settle. Illuminating a sample with a light source and creating a camera image. Evaluating the image for presence of particles within the sample and focus sharpness. Repositioning an objective lens or sample as required. Illuminating a portion of one particle within the sample with a light source having a specified wavelength and measuring the scattered light and emitted fluorescent light utilizing a confocal apparatus with at least one photodetector having specified photosensitive characteristics. Measurement of light characteristics includes laser scanning conformal cytometer. The method includes using a mathematical function derived standard curve to infer the concentration of the analyte.

Abstract: An optical analysis apparatus, including: a sample delivery system from which a liquid sample may be delivered in operation; a flow cell defining a channel through which, in operation, the delivered liquid sample may flow at a controllable rate, the channel including an optical analysis region; an illumination source focused on a portion of the optical analysis region that, in operation, illuminates a single particle at a time in a stream of the sample wider than the single particle; a detector that, in operation, detects light resulting from the illumination of the sample and outputting a signal representative of the detected light; and an analysis system receiving the representative signal.

Abstract: A system for filtering light using a spectrometer enhanced with spectral filters using an array of independent photodetectors to measure the fluorescent or scattered light signal. A system comprising a light source, an illuminated sample, a light spectrum device, a collimator lens, a plurality of spectral filters each having a varying and selected light transmission spectrum and a plurality of photodetectors wherein each photodetector is oriented to a spectral filter. A scanning cytometer for measuring fluorescence and light scattering from an illuminated portion of the sample comprising a first light source, a scanner scanning in two axes, a fluorescence detector, an objective lens, an optically translucent medium through which a sample may be illuminated and a confocal apparatus positioned distally from the light source and sample and through which light signals from the sample are transmitted to a fluorescence detector.

Abstract: In processes and systems utilizing a compressor to fill a vessel with gas, for example, in the filling of the fuel tank of a vehicle that operates on compressed natural gas (CNG), a number of advantages are realized by establishing independent control over the flow rate of gas discharged from the compressor, such as on the basis of the compressor discharge pressure, utilizing feedback from one or more gas pressure sensors. In representative embodiments, a detected or actual measured compressor discharge pressure can serve as a process variable, in a feedback control loop for regulating the output of the compressor.

Abstract: An optical analysis apparatus, including: a sample delivery system from which a liquid sample may be delivered in operation; a flow cell defining a channel through which, in operation, the delivered liquid sample may flow at a controllable rate, the channel including an optical analysis region; an illumination source focused on a portion of the optical analysis region that, in operation, illuminates a single particle at a time in a stream of the sample wider than the single particle; a detector that, in operation, detects light resulting from the illumination of the sample and outputting a signal representative of the detected light; and an analysis system receiving the representative signal.

Abstract: A method for producing diamond grits for use in a wafer slicing system includes adjusting an initial diamond size distribution until an intermediate diamond size distribution is generated. The intermediate diamond size distribution has a corresponding simulated penetration thickness value less than or equal a predetermined penetration thickness value, and penetration thickness is a parameter proportional to a depth of subsurface damage that would occur when slicing an ingot using a diamond coated wire having an associated diamond size distribution. The method may include adjusting the intermediate diamond size distribution until a final diamond size distribution is generated, wherein the final diamond size distribution has a maximum diamond grit size that is substantially equal to a predetermined maximum diamond grit size, and manufacturing the diamond coated wire such that the diamond coated wire has a plurality of diamond grits that fit the final diamond size distribution.

Abstract: A method for designing a diamond coated wire for use in a wafer slicing system includes adjusting an initial diamond size distribution until an intermediate diamond size distribution is generated, wherein the intermediate diamond size distribution has a corresponding simulated penetration thickness value less than or equal a predetermined penetration thickness value, and wherein penetration thickness is a parameter proportional to a depth of subsurface damage that would occur when slicing an ingot using a diamond coated wire having an associated diamond size distribution. The method may include adjusting the intermediate diamond size distribution until a final diamond size distribution is generated, wherein the final diamond size distribution has a maximum diamond grit size that is substantially equal to a predetermined maximum diamond grit size, and manufacturing the diamond coated wire such that the diamond coated wire has a plurality of diamond grits that fit the final diamond size distribution.

Abstract: In processes and systems utilizing a compressor to fill a vessel with gas, for example, in the filling of the fuel tank of a vehicle that operates on compressed natural gas (CNG), a number of advantages are realized by establishing independent control over the flow rate of gas discharged from the compressor, such as on the basis of the compressor discharge pressure, utilizing feedback from one or more gas pressure sensors. In representative embodiments, a detected or actual measured compressor discharge pressure can serve as a process variable, in a feedback control loop for regulating the output of the compressor.

Abstract: In processes and systems utilizing a compressor to fill a vessel with gas, for example, in the filling of the fuel tank of a vehicle that operates on compressed natural gas (CNG), a number of advantages are realized by establishing independent control over the flow rate of gas discharged from the compressor, such as on the basis of the compressor discharge pressure, utilizing feedback from one or more gas pressure sensors. In representative embodiments, a detected or actual measured compressor discharge pressure can serve as a process variable, in a feedback control loop for regulating the output of the compressor.

Abstract: A method for designing a diamond coated wire for use in a wafer slicing system includes adjusting an initial diamond size distribution until an intermediate diamond size distribution is generated, wherein the intermediate diamond size distribution has a corresponding simulated penetration thickness value less than or equal a predetermined penetration thickness value, and wherein penetration thickness is a parameter proportional to a depth of subsurface damage that would occur when slicing an ingot using a diamond coated wire having an associated diamond size distribution. The method may include adjusting the intermediate diamond size distribution until a final diamond size distribution is generated, wherein the final diamond size distribution has a maximum diamond grit size that is substantially equal to a predetermined maximum diamond grit size, and manufacturing the diamond coated wire such that the diamond coated wire has a plurality of diamond grits that fit the final diamond size distribution.

Abstract: In processes and systems utilizing a compressor to fill a vessel with gas, for example, in the filling of the fuel tank of a vehicle that operates on compressed natural gas (CNG), a number of advantages are realized by establishing independent control over the flow rate of gas discharged from the compressor, such as on the basis of the compressor discharge pressure, utilizing feedback from one or more gas pressure sensors. In representative embodiments, a detected or actual measured compressor discharge pressure can serve as a process variable, in a feedback control loop for regulating the output of the compressor.

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

Abstract: Methods for altering one or more parameters of a measurement system are provided. One method includes analyzing a sample using the system to generate values from classification channels of the system for a population of particles in the sample. The method also includes identifying a region in a classification space in which the values for the populations are located. In addition, the method includes determining an optimized classification region for the population using one or more properties of the region. The optimized classification region contains a predetermined percentage of the values for the population. The optimized classification region is used for classification of particles in additional samples.

Abstract: Methods, data structures, and systems for classifying particles are provided. In particular, the methods and systems are configured to acquire a first set of data corresponding to measurable parameters of a microparticle and identify a location of a look-up table to which the first set of data corresponds, wherein the look-up table is framed by values associated with at least one of the measurable parameters. Furthermore, the methods and systems are configured to determine whether the first set of data fits one or more predefined algorithms respectively indicative of a different microparticle classification associated with the identified location of the look-up table. The methods and systems are further configured to classifying the microparticle within at least one predefined categorization based upon the determination of whether the first set of data fits the one or more predefined algorithms.

Abstract: Methods for altering one or more parameters of a measurement system are provided. One method includes analyzing a sample using the system to generate values from classification channels of the system for a population of particles in the sample. The method also includes identifying a region in a classification space in which the values for the populations are located. In addition, the method includes determining an optimized classification region for the population using one or more properties of the region. The optimized classification region contains a predetermined percentage of the values for the population. The optimized classification region is used for classification of particles in additional samples.