Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

Abstract: Methods, systems and apparatus are provided for quantifying the amount of at least a first cell free RNA (cfRNA) present in a liquid biopsy obtained from an individual subject. The first cfRNA may encode a protein that functions in the clearance of xenobiotic compounds from the body of the subject. Quantification of the amount of the first cfRNA is normalised to the individual and permits the construction of more accurate virtual models that facilitate improved personalised medicine, dosage regimens and clinical trials.

Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

Abstract: The present invention relates to functionally closed systems for mixing and delivering microbubble pharmaceuticals. Described is a device comprising a rigid outer layer and a flexible bladder wherein mixing occurs using external mixing sources. Also included is a system for dispensing the pharmaceuticals to its end use after mixing. Methods for mixing and dispensing microbubble pharmaceuticals are also described.

Abstract: The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

Abstract: The present invention relates to functionally closed systems for mixing and delivering microbubble pharmaceuticals. Described is a device comprising a rigid outer layer and a flexible bladder wherein mixing occurs using external mixing sources. Also included is a system for dispensing the pharmaceuticals to its end use after mixing. Methods for mixing and dispensing microbubble pharmaceuticals are also described.

Abstract: A shaft assembly includes a metallic portion and a ceramic portion. In a disclosed example, a ceramic shaft has a generally conical end that is at least partially received within a generally conical recess in one end of a metallic shaft. The recess has a first portion that has an inner surface that is received directly against a corresponding outer surface on a section of the ceramic shaft conical portion. A second portion of the recess is radially spaced from the ceramic shaft and provides a controlled gap within which a braze alloy is maintained. In one example, the second portion has an axial length that is more than twice a radial depth of the second portion. In another example, a transition surface between the first and second portions of the recess has a linear profile.

Abstract: The present invention relates to methods and compositions for delivery of a compound, most preferably a polynucleotide, into the cytoplasm of a cell by means of a microparticle, fabricated from a pH-sensitive hydrogel in collapsed phase, and having a size and physical characteristics compatible with uptake via a clathrin-coated pit on the cell surface. The trigger pH at which the hydrogel expands is lower than the physiological pH of the extracellular environment, allowing the microparticle, which comprises the compound to be delivered, to maintain its compact size prior to cellular uptake, thereby providing the additional benefit of protecting the comprised compound from degradation. This protective feature is particularly beneficial in embodiments in which a polynucleotide or a peptide is being delivered. Following uptake via a clathrin-coated pit, the microparticle then enters the intracellular endocytic pathway, where it is subjected to a progressive decrease in pH.

Abstract: Improved prop-fan stability is achieved by providing each blade of the prop-fan with a leading edge which, outwardly, from a location thereon at the mid-span of the blade, occupy generally a single plane.

Abstract: A structural composite panel comprises a pair of filament-reinforced composite facing sheets and a ruffled, corrugated core secured between the facing sheets. The core has corrugations each with an axis having periodic oscillations in plane of the core and with the axis generally normal to filament direction.

Abstract: A large wind turbine rotor blade having a filament wound composite spar securely mounted to a support structure in a manner by which blade loads are transmitted via redundant load paths. Concentric inner and outer metallic adapter sleeves are bonded to the inboard end of the blade spar, and the adapter sleeves and blade spar are joined by a first series of shear bolts and nuts which pass radially through the spar. A second series of radially extending bolts and nuts join the inner and outer sleeves slightly inboard of the end of the spar. The end of the spar is of slightly reduced diameter forming a conical surface which interacts with the inner and outer sleeves to produce a positive lock when the blade is subject to centrifugal loads. The outer sleeve is adapted to be connected to the support structure by conventional techniques such as by a plurality of bolts which extend axially into the butt end of the outer sleeve.

Abstract: A fan blade having a breakaway leading edge positioned on the spar and shell that constitute the main structure of the blade, the leading edge being adapted to break away, in part at least, upon being struck by a large foreign object, with the shell providing an acceptable leading edge after the breakaway.

Abstract: A filament-reinforced composite article is fabricated from monolayer boron fiber tapes and laminates of titanium. The tapes which have the boron fibers attached to an aluminum foil are positioned in a multi-ply layup with interleaved titanium laminates in the form of foils or screening. The layup is subjected to heat and pressure in a diffusion bonding process to compact the layup materials and bond the fibers in the matrix of aluminum and titanium. The titanium in the matrix improves the impact load resistance of the article by improving ductility and impact strength.