Abstract: This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant density, and by affinity purification.

Abstract: The present invention is directed, in general, to a chemical mechanical polishing pad comprising a closed-cell thermoplastic foam polishing body. The polishing body comprises an ethylene vinyl acetate block copolymer. The ethylene vinyl acetate block copolymer comprises a vinyl acetate content ranging from about 1 to about 18 wt %. The closed-cell thermoplastic foam polishing body also comprises filler particles comprising an average size ranging from about 1 to about 20 microns. Other aspects of the invention comprise a method for manufacturing the above-described chemical mechanical polishing pad and chemical mechanical polishing apparatus comprising the chemical mechanical polishing pad.

Abstract: This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector comprising a heterologous (i.e. non-AAV) transgene of interest, and a helper virus such as an adenovirus. The AAV vector preparations produced are generally replication incompetent but are capable of mediating delivery of a transgene of interest (such as a therapeutic gene) to any of a wide variety of tissues and cells. The AAV vector preparations produced according to this invention are also substantially free of helper virus as well as helper viral and cellular proteins and other contaminants. The invention described herein provides methods of producing rAAV particles by culturing producer cells under conditions, such as temperature and pH, that promote release of virus.

Abstract: This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant density, and by affinity purification.

Abstract: The present invention provides a brush seal having upstream and downstream plates that sandwich therebetween a plurality of bristles that sealingly contact a rotating member. The downstream plate includes primary and secondary backing rings with a flexible connection extending between the two rings so as to permit relative radial movement therebetween.

Abstract: A multiple stage brush seal having a controlled distribution of pressure drops across each stage of the seal is provided. The pressure drop across each stage is controlled by one or a combination of the following structural arrangements: increased clearance gaps, reduced bristle packing, and venting holes in the seal stage backing plate.

Abstract: A bearing assembly includes a split inner race having first and second inner race halves joined together at a split line. A plurality of center feed passages are provided at the split line, and at least one radial pilot passage is provided in one of the inner race halves and axially spaced from the center feed passages. A bifurcated distribution manifold is disposed at the split line and includes a center distribution manifold disposed in flow communication with the center feed passages, and a pilot distribution manifold disposed in flow communication with solely the pilot passage. The bifurcated distribution manifold ensures the independent channeling of lubricating oil to both the center feed passages and the pilot passage under centrifugal force upon rotation of the inner race.

Abstract: A dry rolling element bearing assembly for rotatably mounting a flight vehicle engine shaft to a housing. Rolling elements (such as ball bearings) are positioned between inner and outer races. Stop devices axial position the inner and outer races. A mounting device positions the inner race radially outward from the shaft so as to create a first annular air space between them. The mount has a flexible portion and has a support ring portion which contacts the outer surface of the inner race with a radial interference fit. A similar mounting device positions the outer race radially inward from the housing. Air directing devices direct cooling air impingingly against bearing surfaces.