Patents by Inventor Edward B. Watson, III

Edward B. Watson, III has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Processes for purification of recombinant proteins
    Patent number: 7741053
    Abstract: Processes for the detection and purification of peptides and proteins comprising a metal ion-affinity peptide having a high affinity for coordinated metals and also being highly antigenic are described. Antibodies for use in the processes are also described.
    Type: Grant
    Filed: May 13, 2005
    Date of Patent: June 22, 2010
    Assignee: Sigma-Aldrich Co.
    Inventors: Richard J. Mehigh, Eliezer Kopf, Efrat Reem, Edward B. Watson, III
  • Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
    Patent number: 7438806
    Abstract: An affinity matrix for use in affinity based molecular pull down and immunoprecipitation procedures. The affinity matrix comprises a polymeric support, a dye attached to a fraction of the polymeric support to enable optical detection of the polymeric support, and an affinity ligand other than the dye attached to a fraction of the polymeric support for the capture of a molecule. Also provided is a method for the isolation of a biomolecule from an aqueous solution. The method comprises combining the aqueous solution with an affinity matrix comprising a polymeric support and separating the affinity matrix from the aqueous solution. A dye is attached to a fraction of the polymeric support which enables optical detection and monitoring of the affinity matrix and, accordingly, reduces the likelihood of the loss of affinity matrix during the separation step. In addition, an affinity ligand other than the dye is also attached to a fraction of the polymeric support for the capture of the biomolecule.
    Type: Grant
    Filed: November 2, 2006
    Date of Patent: October 21, 2008
    Assignee: Sigma-Aldrich Co.
    Inventors: Edward B. Watson, III, Kenneth E. Heuermann, John G. Dapron
  • Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
    Patent number: 7163633
    Abstract: An affinity matrix for use in affinity based molecular pull down and immunoprecipitation procedures. The affinity matrix comprises a polymeric support, a dye attached to a fraction of the polymeric support to enable optical detection of the polymeric support, and an affinity ligand other than the dye attached to a fraction of the polymeric support for the capture of a molecule. Also provided is a method for the isolation of a biomolecule from an aqueous solution. The method comprises combining the aqueous solution with an affinity matrix comprising a polymeric support and separating the affinity matrix from the aqueous solution. A dye is attached to a fraction of the polymeric support which enables optical detection and monitoring of the affinity matrix and, accordingly, reduces the likelihood of the loss of affinity matrix during the separation step. In addition, an affinity ligand other than the dye is also attached to a fraction of the polymeric support for the capture of the biomolecule.
    Type: Grant
    Filed: October 1, 2004
    Date of Patent: January 16, 2007
    Assignee: Sigma-Aldrich Co.
    Inventors: Edward B. Watson, III, Kenneth E. Heuermann, John G. Dapron
  • Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
    Patent number: 6887377
    Abstract: An affinity matrix for use in affinity based molecular pull down and immunoprecipitation procedures. The affinity matrix comprises a polymeric support, a dye attached to a fraction of the polymeric support to enable optical detection of the polymeric support, and an affinity ligand other than the dye attached to a fraction of the polymeric support for the capture of a molecule. Also provided is a method for the isolation of a biomolecule from an aqueous solution. The method comprises combining the aqueous solution with an affinity matrix comprising a polymeric support and separating the affinity matrix from the aqueous solution. A dye is attached to a fraction of the polymeric support which enables optical detection and monitoring of the affinity matrix and, accordingly, reduces the likelihood of the loss of affinity matrix during the separation step. In addition, an affinity ligand other than the dye is also attached to a fraction of the polymeric support for the capture of the biomolecule.
    Type: Grant
    Filed: January 31, 2002
    Date of Patent: May 3, 2005
    Assignee: Sigma-Aldrich Co.
    Inventors: Edward B. Watson, III, Kenneth E. Heuermann, John G. Dapron