Abstract: A high-density distributed three-dimensional electrode device and an associated electroporation method is provided. The method includes applying an electric pulse of a first polarity to a first group of electrodes while simultaneously applying an electrical pulse of a second polarity to a remaining group of electrodes, and then applying an electric pulse of the first polarity on a second group of electrodes while simultaneously applying an electric pulse of the second polarity to the remaining groups of electrodes. The electrodes receiving the electric pulse of the first polarity being surrounded by the electrodes receiving the electric pulse of the second polarity, and the first polarity and the second polarity are opposite.

Abstract: Provided is a flow electroporation device, including a bracket and an electrode device, wherein the electrode device is planar electrode, and the bracket and the planar electrodes jointly restrict the channel for fluid flow; the material of the electrode device is one or several of following materials: titanium-plated platinum, platinum, rhodium-plated molybdenum, rhodium-plated ruthenium, iridium-plated niobium and platinum-plated iridium; the making process of the flow electroporation device is simple and the product can be manufactured in a standardized process. Compared to other similar devices, the single batch processing volume of cell suspension is larger, the transfection efficiency and the cell viability are higher, the stability is better, and the device is easy to operate.

Abstract: A flow electrotransfection device with a chamber for fluid to flow though, a liquid inlet channel for communicating with the chamber and a liquid outlet channel for communicating with the chamber, includes an electrode module for applying an electric field to fluid in the chamber, and further includes a fluid guiding structure for making flow velocities of fluid flowing through the central zone and the peripheral zone of the chamber tend to be consistent. The present disclosure solves the technical problem of relatively uneven the flow velocities of fluid at different zones in the flow electrotransfection chamber, improves the accuracy of the number of electric shock when the fluid flows though the chamber, thus improving the electrotransfection efficiency, and reduces the cell damage and even death from excessive times of electric shock, thereby improving cell viability.

Abstract: An enclosed centrifugation device is provided, including an integrated tube body that includes an upper part, a middle part, and a lower part, wherein the upper part is configured to have multiple openings each having an open state and a closed state. The openings that are used for sealed connection to external tubing, include the first opening serving as a liquid inlet and the second opening serving as a liquid outlet. Also provided is a method for segregating cells. The enclosed centrifugation device provided by the present invention can centrifuge cell suspension and realize rapid inlet, outlet, or replacement of liquid in an enclosed setting, which greatly reduces the risk of being contaminated for biological sample, and simultaneously meets the requirement by regulations that sample treatment by Class III medical devices must be fully enclosed.

Abstract: A cell clump dispersing device for dispersing cell clumps is provided, including a dispersion structure module that includes at least one structure for providing fluid flow shear force. Each structure for providing liquid flow shear force includes an inlet for cell clumps to be dispersed to enter the structure, and at least one outlet for dispersed cell clumps to flow out of the structure. A diameter of the outlet is smaller than that of the inlet, and an end of at least one of the structure is capable of being connected to an end of another of the structure.

Abstract: A high-density distributed three-dimensional electrode device and an associated electroporation method is provided. The method includes applying an electric pulse of a first polarity to a first group of electrodes while simultaneously applying an electrical pulse of a second polarity to a remaining group of electrodes, and then applying an electric pulse of the first polarity on a second group of electrodes while simultaneously applying an electric pulse of the second polarity to the remaining groups of electrodes. The electrodes receiving the electric pulse of the first polarity being surrounded by the electrodes receiving the electric pulse of the second polarity, and the first polarity and the second polarity are opposite.

Abstract: Disclosed herein is a high-density distributed three-dimensional electrode device and an associated electroporation method. The method includes applying an electric pulse of a first polarity to a first group of electrodes while simultaneously applying an electrical pulse of a second polarity to a remaining group of electrodes, and then applying an electric pulse of the first polarity on a second group of electrodes while simultaneously applying an electric pulse of the second polarity to the remaining groups of electrodes. The electrodes receiving the electric pulse of the first polarity being surrounded by the electrodes receiving the electric pulse of the second polarity, and the first polarity and the second polarity are opposite.

Abstract: The present invention discloses a flow electroporation device comprising a three-dimensional electrode device and a flow electroporation chamber, the three-dimensional electrode device comprising an electrode array and an electrode fixing assembly for fixing electrodes in the electrode array, the flow electroporation chamber comprising a cavity, an inlet, an outlet and an opening, the inlet, the outlet and the opening on the cavity, and the electrode array being inserted into the cavity through the opening. By means of the above-mentioned manner, the flow electroporation device of the present invention can achieve a high throughput cell treatment, and also can work under a low voltage condition, can avoid damages to cells brought by liquid flow, can reduce effects brought by heat and cathode effect induced by electric pulses, and can maintain an closed aseptic environment.

Abstract: The present invention discloses a flow electroporation device comprising a three-dimensional electrode device and a flow electroporation chamber, the three-dimensional electrode device comprising an electrode array and an electrode fixing assembly for fixing electrodes in the electrode array, the flow electroporation chamber comprising a cavity, an inlet, an outlet and an opening, the inlet, the outlet and the opening on the cavity, and the electrode array being inserted into the cavity through the opening. By means of the above-mentioned manner, the flow electroporation device of the present invention can achieve a high throughput cell treatment, and also can work under a low voltage condition, can avoid damages to cells brought by liquid flow, can reduce effects brought by heat and cathode effect induced by electric pulses, and can maintain an closed aseptic environment.

Abstract: Provided is a flow electroporation device, including a bracket and an electrode device, wherein the electrode device is planar electrode, and the bracket and the planar electrodes jointly restrict the channel for fluid flow; the material of the electrode device is one or several of following materials: titanium-plated platinum, platinum, rhodium-plated molybdenum, rhodium-plated ruthenium, iridium-plated niobium and platinum-plated iridium; the making process of the flow electroporation device is simple and the product can be manufactured in a standardized process. Compared to other similar devices, the single batch processing volume of cell suspension is larger, the transfection efficiency and the cell viability are higher, the stability is better, and the device is easy to operate.

Abstract: The present invention discloses a flow electroporation device comprising a three-dimensional electrode device and a flow electroporation chamber, the three-dimensional electrode device comprising an electrode array and an electrode fixing assembly for fixing electrodes in the electrode array, the flow electroporation chamber comprising a cavity, an inlet, an outlet and an opening, the inlet, the outlet and the opening on the cavity, and the electrode array being inserted into the cavity through the opening By means of the above-mentioned manner, the flow electroporation device of the present invention can achieve a high throughput cell treatment, and also can work under a low voltage condition, can avoid damages to cells brought by liquid flow, can reduce effects brought by heat and cathode effect induced by electric pulses, and can maintain an closed aseptic environment.

Abstract: Disclosed herein is a high-density distributed three-dimensional electrode device and an associated electroporation method. The method includes applying an electric pulse of a first polarity to a first group of electrodes while simultaneously applying an electrical pulse of a second polarity to a remaining group of electrodes, and then applying an electric pulse of the first polarity on a second group of electrodes while simultaneously applying an electric pulse of the second polarity to the remaining groups of electrodes. The electrodes receiving the electric pulse of the first polarity being surrounded by the electrodes receiving the electric pulse of the second polarity, and the first polarity and the second polarity are opposite.

Abstract: An electroporating drug delivering device using hollow needle electrode includes a drug delivering head, a control console as well as a control line and a tube for connecting the drug delivering head and the control console. The drug delivering head includes a universal joint, a camera, an illuminating lamp, a molecular drug delivery structure, an electric connector, a grille, a hollow needle electrode array, and a stretchable structure. The control console includes a display for observing images, a rocker for controlling the universal joint, and a remote starting unit for controlling the generation of electric pulses. The control console is connected with the electric connector inside of the drug delivering head by the line arranged inside of the tube. The electroporating drug-administration device enhances the drug utilization efficiency and reduces the treatment time, and can accurately adjust the intensity and region of the electric field.

Abstract: An electroporating drug delivering device using hollow needle electrode includes a drug delivering head, a control console as well as a control line and a tube for connecting the drug delivering head and the control console. The drug delivering head includes a universal joint, a camera, an illuminating lamp, a molecular drug delivery structure, an electric connector, a grille, a hollow needle electrode array, and a stretchable structure. The control console includes a display for observing images, a rocker for controlling the universal joint, and a remote starting unit for controlling the generation of electric pulses. The control console is connected with the electric connector inside of the drug delivering head by the line arranged inside of the tube. The electroporating drug-administration device enhances the drug utilization efficiency and reduces the treatment time, and can accurately adjust the intensity and region of the electric field.

Abstract: The present invention discloses a flow electroporation device comprising a three-dimensional electrode device and a flow electroporation chamber, the three-dimensional electrode device comprising an electrode array and an electrode fixing assembly for fixing electrodes in the electrode array, the flow electroporation chamber comprising a cavity, an inlet, an outlet and an opening, the inlet, the outlet and the opening on the cavity, and the electrode array being inserted into the cavity through the opening By means of the above-mentioned manner, the flow electroporation device of the present invention can achieve a high throughput cell treatment, and also can work under a low voltage condition, can avoid damages to cells brought by liquid flow, can reduce effects brought by heat and cathode effect induced by electric pulses, and can maintain an closed aseptic environment.

Abstract: A high-density distributed three-dimensional electrode device, comprising an electrode array and an electrode fixing assembly on which the electrode array is fixed, the electrode array comprising a plurality of electrodes respectively applied with an electric pulse of a first polarity and an electric pulse of a second polarity according to a time period, wherein the electrodes corresponding to the electric pulse of the second polarity are distributed around the electrodes corresponding to the electric pulse of the first polarity.