Abstract: The disclosure features novel lipids and compositions involving the same. Lipid nanoparticles (e.g., empty LNPs or loaded LNPs) include a novel lipid as well as additional lipids such as phospholipids, structural lipids, and PEG lipids. Lipid nanoparticles (e.g., empty LNPs or loaded LNPs) further including therapeutic and/or prophylactics such as RNA are useful in the delivery of therapeutic and/or prophylactics to mammalian cells or organs to, for example, regulate polypeptide, protein, or gene expression.

Abstract: An electronic gaming machine and a method are provided which generates outcome presentations from a Class 2 or a central determination game outcome. A database stores RNG seeds that correspond to various presentations. To generate the outcome presentations, the RNG seeds are retrieved from the database and random numbers are generated from the RNG seeds. These random numbers are then used to generate the outcome presentations for the Class 2 or central determination game outcome. The RNG seeds may be stored as a series of RNG seeds which may be individually accessed to eliminate certain outcomes or sort certain outcomes prior to presentation.

Abstract: The disclosure features novel lipids and compositions involving the same. Nanoparticle compositions include a novel lipid as well as additional lipids such as phospholipids, structural lipids, and PEG lipids. Nanoparticle compositions further including therapeutic and/or prophylactics such as RNA are useful in the delivery of therapeutic and/or prophylactics to mammalian cells or organs to, for example, regulate polypeptide, protein, or gene expression.

Abstract: An electronic gaming machine and a method are provided which generates outcome presentations from a Class 2 or a central determination game outcome. A database stores RNG seeds that correspond to various presentations. To generate the outcome presentations, the RNG seeds are retrieved from the database and random numbers are generated from the RNG seeds. These random numbers are then used to generate the outcome presentations for the Class 2 or central determination game outcome. The RNG seeds may be stored as a series of RNG seeds which may be individually accessed to eliminate certain outcomes or sort certain outcomes prior to presentation.

Abstract: A system includes a display device including a display surface configured to display a graphic and including an opening. The system also includes a peripheral device mounted within the opening of the display surface, and a processor configured to execute instructions stored in a memory device, which when executed, cause the processor to at least: display the graphic on the display surface of the display device adjacent to the peripheral device, whereby the graphic is displayed at least partially in association with the peripheral device.

Abstract: A system includes a display device including a display surface configured to display a graphic and including an opening. The system also includes a peripheral device mounted within the opening of the display surface, and a processor configured to execute instructions stored in a memory device, which when executed, cause the processor to at least: display the graphic on the display surface of the display device adjacent to the peripheral device, whereby the graphic is displayed at least partially in association with the peripheral device.

Abstract: This disclosure relates to messenger RNA (mRNA) therapy for the treatment of methylmalonic acidemia. mRNAs for use in the invention, when administered in vivo, encode methylmalonyl-CoA mutase. mRNA therapies of the disclosure increase and/or restore deficient levels of MUT expression and/or activity in subjects.

Abstract: This disclosure relates to ionizable amino lipid-based lipid nanoparticles for delivery of mRNA encoding propionyl-CoA carboxylase alpha (PCCA) and propionyl-CoA carboxylase beta (PCCB). Lipid nanoparticle/mRNA therapies of the invention increase and/or restore deficient levels of PCCA and/or PCCB expression and/or activity in subjects and are useful for the treatment of propionic academia.

Abstract: This disclosure relates to ionizable amino lipid-based lipid nanoparticles for delivery of mRNA encoding glucose-6-phosphatase. Lipid nanoparticle/mRNA therapies of the invention increase and/or restore deficient levels of glucose-6-phosphatase expression and/or activity in subjects and are useful for the treatment of glycogen storage disease type 1a (GSD-Ia). Lipid nanoparticle/mRNA therapies of the invention increase glucose production and reduce the abnormal accumulation of glycogen and glucose-6-phosphate associated with GSD-Ia.

Abstract: This disclosure relates to ionizable amino lipid-based lipid nanoparticles for delivery of mRNA encoding phenylalanine hydroxylase.

Abstract: This disclosure relates to mRNA therapy for the treatment of ornithine transcarbamylase deficiency (OTCD). mRNAs for use in the invention, when administered in vivo, encode human ornithine transcarbamylase (OTC). mRNA therapies of the disclosure increase and/or restore deficient levels of OTC expression and/or activity in subjects. mRNA therapies of the disclosure further decrease levels of toxic ammonia associated with deficient OTC activity in subjects.

Abstract: An electronic gaming machine and a method are provided which generates outcome presentations from a Class 2 or a central determination game outcome. A database stores RNG seeds that correspond to various presentations. To generate the outcome presentations, the RNG seeds are retrieved from the database and random numbers are generated from the RNG seeds. These random numbers are then used to generate the outcome presentations for the Class 2 or central determination game outcome. The RNG seeds may be stored as a series of RNG seeds which may be individually accessed to eliminate certain outcomes or sort certain outcomes prior to presentation.

Abstract: An electronic gaming machine and a method are provided which generates outcome presentations from a Class 2 or a central determination game outcome. A database stores RNG seeds that correspond to various presentations. To generate the outcome presentations, the RNG seeds are retrieved from the database and random numbers are generated from the RNG seeds. These random numbers are then used to generate the outcome presentations for the Class 2 or central determination game outcome. The RNG seeds may be stored as a series of RNG seeds which may be individually accessed to eliminate certain outcomes or sort certain outcomes prior to presentation.

Abstract: The disclosure features novel lipids and compositions involving the same. Lipid nanoparticles (e.g., empty LNPs or loaded LNPs) include a novel lipid as well as additional lipids such as phospholipids, structural lipids, and PEG lipids. Lipid nanoparticles (e.g., empty LNPs or loaded LNPs) further including therapeutic and/or prophylactics such as RNA are useful in the delivery of therapeutic and/or prophylactics to mammalian cells or organs to, for example, regulate polypeptide, protein, or gene expression.

Abstract: The disclosure features novel lipids and compositions involving the same. Lipid nanoparticles (e.g., empty LNPs or loaded LNPs) include a novel lipid as well as additional lipids such as phospholipids, structural lipids, and PEG lipids. Lipid nanoparticles (e.g., empty LNPs or loaded LNPs) further including therapeutic and/or prophylactics such as RNA are useful in the delivery of therapeutic and/or prophylactics to mammalian cells or organs to, for example, regulate polypeptide, protein, or gene expression.

Abstract: An electronic gaming machine and a method are provided which generates outcome presentations from a Class 2 or a central determination game outcome. A database stores RNG seeds that correspond to various presentations. To generate the outcome presentations, the RNG seeds are retrieved from the database and random numbers are generated from the RNG seeds. These random numbers are then used to generate the outcome presentations for the Class 2 or central determination game outcome. The RNG seeds may be stored as a series of RNG seeds which may be individually accessed to eliminate certain outcomes or sort certain outcomes prior to presentation.

Abstract: The disclosure features target cell delivery lipid nanoparticle (LNP) compositions that allow for enhanced delivery of agents, e.g., nucleic acids, such as therapeutic and/or prophylactic RNAs, to target cells, in particular liver cells and/or splenic cells. The LNPs comprise an effective amount of a target cell delivery potentiating lipid such that delivery of an agent by a target cell target cell delivery LNP is enhanced as compared to an LNP lacking the target cell delivery potentiating agent. Methods of using the target cell target cell delivery LNPs for delivery of agents, e.g., nucleic acid delivery, for protein expression, and for modulating target cell activity are also disclosed.

Abstract: An electronic gaming machine and a method are provided which generates outcome presentations from a Class 2 or a central determination game outcome. A database stores RNG seeds that correspond to various presentations. To generate the outcome presentations, the RNG seeds are retrieved from the database and random numbers are generated from the RNG seeds. These random numbers are then used to generate the outcome presentations for the Class 2 or central determination game outcome. The RNG seeds may be stored as a series of RNG seeds which may be individually accessed to eliminate certain outcomes or sort certain outcomes prior to presentation.

Abstract: An electronic gaming machine and a method are provided which generates outcome presentations from a Class 2 or a central determination game outcome. A database stores RNG seeds that correspond to various presentations. To generate the outcome presentations, the RNG seeds are retrieved from the database and random numbers are generated from the RNG seeds. These random numbers are then used to generate the outcome presentations for the Class 2 or central determination game outcome. The RNG seeds may be stored as a series of RNG seeds which may be individually accessed to eliminate certain outcomes or sort certain outcomes prior to presentation.

Abstract: A system includes a display device including a display surface configured to display a graphic and including an opening. The system also includes a peripheral device mounted within the opening of the display surface, and a processor configured to execute instructions stored in a memory device, which when executed, cause the processor to at least: display the graphic on the display surface of the display device adjacent to the peripheral device, whereby the graphic is displayed at least partially in association with the peripheral device.