Abstract: Embodiments described herein generally relate to assemblies, systems, and methods for cell culture and production of biologics. Disclosed herein are multi-purpose assemblies that facilitate combination of perfusion processes and harvest processes. The multi-purpose assemblies and systems comprising the same simplify and streamline the biomanufacturing process, at least by improving automation capabilities, reducing physical footprint, reducing risk of contamination, and reducing consumable use rates. Also disclosed herein are methods of using the aforementioned assemblies and systems.