Patents by Inventor Edward J. Bylina
Edward J. Bylina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9243236Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organizms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: GrantFiled: May 5, 2014Date of Patent: January 26, 2016Assignee: BP Corporation North America Inc.Inventors: Edward J. Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Publication number: 20140323356Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: ApplicationFiled: May 5, 2014Publication date: October 30, 2014Applicant: BP Corporation North America Inc.Inventors: Edward J. Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Patent number: 8715993Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: GrantFiled: July 13, 2012Date of Patent: May 6, 2014Assignee: BP Corporation North America Inc.Inventors: Edward J. Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Publication number: 20120276078Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: ApplicationFiled: July 13, 2012Publication date: November 1, 2012Applicant: BP Corporation North America Inc.Inventors: Edward J. Bylina, Ronald Swanson, Eric Mathur, David E. Lam
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Publication number: 20120141453Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: ApplicationFiled: February 15, 2012Publication date: June 7, 2012Applicant: BP Corporation North America Inc.Inventors: Edward J. Bylina, Ronald Swanson, Eric Mathur, David E. Lam
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Patent number: 8119383Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: GrantFiled: August 20, 2010Date of Patent: February 21, 2012Assignee: BP Corporation North America Inc.Inventors: Edward J. Bylina, Ronald Swanson, Eric Mathur, David E. Lam
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Publication number: 20100311845Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: ApplicationFiled: August 20, 2010Publication date: December 9, 2010Inventors: EDWARD J. BYLINA, RONALD SWANSON, ERIC MATHUR, DAVID E. LAM
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Patent number: 7807433Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: GrantFiled: October 2, 2007Date of Patent: October 5, 2010Assignee: Verenium CorporationInventors: Edward J. Bylina, Ronald Swanson, Eric Mathur, David E. Lam
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Publication number: 20090142326Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: ApplicationFiled: October 2, 2007Publication date: June 4, 2009Inventors: Edward J. Bylina, Ronald Swanson, Eric Mathur, David E. Lam
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Patent number: 7379601Abstract: Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources.Type: GrantFiled: May 17, 2007Date of Patent: May 27, 2008Assignee: Kairos-Scientific Inc.Inventors: Mary Mei-Iing Yang, Edward J. Bylina, William Joseph Coleman, Michael Robert Dilworth, Steven J. Robles, Douglas Charles Youvan
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Patent number: 7294498Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: GrantFiled: April 9, 2002Date of Patent: November 13, 2007Assignee: Verenium CorporationInventors: Edward J. Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Patent number: 6834122Abstract: Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources.Type: GrantFiled: January 22, 2001Date of Patent: December 21, 2004Assignee: Kairos Scientific, Inc.Inventors: Mary M. Yang, Edward J. Bylina, William J. Coleman, Michael R. Dilworth, Steven J. Robles, Douglas C. Youvan
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Patent number: 6661909Abstract: Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red).Type: GrantFiled: July 23, 2001Date of Patent: December 9, 2003Assignee: Kairos Scientific, Inc.Inventors: Dougalas C. Youvan, Christopher M. Silva, Edward J. Bylina, William J. Coleman, Michael R. Dilworth, Mary M. Yang
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Patent number: 6498026Abstract: The present invention provides mutant genes encoding variant galactose oxidase enzymes, termed vGOs; constructs and recombinant host cells incorporating the genes; the vGO polypeptides encoded by the genes; antibodies to the polypeptides; and methods of making and using all of the foregoing.Type: GrantFiled: February 14, 2001Date of Patent: December 24, 2002Assignee: Hercules IncorporatedInventors: Simon Delagrave, Anthony M. Maffia, III, Dennis J. Murphy, Jennifer L. Rittenhouse Pruss, Edward J. Bylina, William J. Coleman
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Patent number: 6472163Abstract: Improvements in calorimetric assays, surfaces for arraying microcolonies and instrument hardware for screening mutagenized enzymes and proteins in a solid phase format are presented. These improvements permit new enzyme activities to be screened. New filter membrane materials and formats for arraying the microcolonies provide higher throughput, better solvent resistance and ease of handling. Modifications to the instrument heating and illumination systems provide improved temperature control and a more compact, folded light path.Type: GrantFiled: March 7, 2000Date of Patent: October 29, 2002Assignee: Kairos Scientific, Inc.Inventors: William J. Coleman, Edward J. Bylina, Douglas C. Youvan
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Publication number: 20020155550Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: ApplicationFiled: April 9, 2002Publication date: October 24, 2002Inventors: Edward J. Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Patent number: 6456734Abstract: Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red).Type: GrantFiled: June 5, 1998Date of Patent: September 24, 2002Assignee: Kairos Scientific, Inc.Inventors: Douglas C. Youvan, Christopher M. Silva, Edward J. Bylina, William J. Coleman, Michael R. Dilworth, Mary M. Yang
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Publication number: 20020118870Abstract: Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red).Type: ApplicationFiled: July 23, 2001Publication date: August 29, 2002Inventors: Dougalas C. Youvan, Christopher M. Silva, Edward J. Bylina, William J. Coleman, Michael R. Dilworth, Mary M. Yang
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Patent number: 6368844Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.Type: GrantFiled: August 13, 1998Date of Patent: April 9, 2002Assignee: Diversa CorporationInventor: Edward J. Bylina
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Publication number: 20020025537Abstract: The invention relates generally to the area of drug development and more specifically to screening novel compounds for antimicrobial activity. Methods are described for generating mutagenized peptide libraries expressed in colonies of cells. A fluorescent live/dead cell assay combined with a digital imaging spectrophotometer provides a high-throughput solid-phase screening method for colonies or arrayed synthetic libraries. The assay enables screening of antimicrobial peptide activity in at least 105-106 colonies per experiment. These methods generate and identify antimicrobial compounds.Type: ApplicationFiled: July 19, 2001Publication date: February 28, 2002Inventors: Edward J. Bylina, William J. Coleman, Douglas C. Youvan