Abstract: In alternative embodiments, provided are immunohistochemistry (IHC) methods and kits for determining and scoring reproducibly the extent of expression of the protein tyrosine-protein kinase-like 7 (PTK7), also known as: colon carcinoma kinase 4 (CCK4); HER2 or receptor tyrosine-protein kinase erbB-2, or cluster of differentiation 340 (CD340); programmed death-ligand 1 (PD-L1), or cluster of differentiation 274 (CD274); B7 homolog 1 (B7-H1); and, Ki-67 or MKI67 (Marker of Proliferation Ki-67), in a tissue sample. In alternative embodiments, provided are methods and kits for diagnosing or selecting an individual eligible for treatment with a cancer or tumor therapeutic, or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein.