Patents by Inventor Edward
Marcotte
Edward Marcotte has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240125805Abstract: Provided herein are methods for analyzing polypeptides and polypeptide complexes. Methods of the present disclosure may be used to identify protein subunits present in a polypeptide, polypeptide complex, or aggregate. These methods may also be used to quantify the subunits (e.g. number of repeating units, protein monomers, repeating domains) in a polypeptide, polypeptide complex, or aggregate.Type: ApplicationFiled: February 24, 2022Publication date: April 18, 2024Inventors: Edward MARCOTTE, Eric ANSLYN, Jagannath SWAMINATHAN, Angela M. BARDO
-
Publication number: 20240002925Abstract: Provided herein are compositions, systems, methods, and kits for peptide analysis, including peptide sequencing. Aspects of the present disclosure provide bifunctional reagents which may selectively couple to amino acids and selectively couple to detectable species. Aspects of the present disclosure further provide methods for using these bifunctional reagents to sequence and analyze peptides.Type: ApplicationFiled: November 18, 2022Publication date: January 4, 2024Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Eric V. ANSLYN, Edward MARCOTTE, Cecil J. HOWARD, II, Jagannath SWAMINATHAN, Angela M. BARDO, Brendan FLOYD, Brandon HOSFORD, Le ZHANG, Emily Faith BABCOCK, Caroline M. HINSON
-
Publication number: 20230107647Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: July 7, 2022Publication date: April 6, 2023Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN
-
Publication number: 20230103041Abstract: The present disclosure provides methods of identifying and quantifying the peptides displayed by the major histocompatibility complex (MHC). Such methods may comprise the ability to determine the type, identity, and quantity of each peptide displayed by the MHC. In some embodiments, these methods may be used to develop an anti-cancer therapy or type the HLA of a patient. Also provided herein are compositions comprising peptides from the MHC which have been prepared for sequencing.Type: ApplicationFiled: October 27, 2022Publication date: March 30, 2023Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward MARCOTTE, Eric ANSLYN, Alexander BOULGAKOV, Angela M. BARDO, Siyuan Stella WANG, Jagannath SWAMINATHAN, Fan TU
-
Publication number: 20230076975Abstract: Described herein are methods for selectively cleaving the C-terminal amino acid of a peptide or protein. The methods described herein may be applicable for, for example, single-molecule peptide or protein sequencing.Type: ApplicationFiled: August 18, 2022Publication date: March 9, 2023Inventors: Eric V. ANSLYN, Edward MARCOTTE, Cecil J. HOWARD, II, Jagannath SWAMINATHAN, Angela M. BARDO, Caroline M. HINSON, Brendan FLOYD, Le ZHANG
-
Patent number: 11435358Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: GrantFiled: October 1, 2021Date of Patent: September 6, 2022Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan
-
Publication number: 20220163536Abstract: The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: July 23, 2021Publication date: May 26, 2022Inventors: Edward MARCOTTE, Jagannath SWAMINATHAN, Andrew ELLINGTON, Eric ANSLYN
-
Publication number: 20220091130Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: October 1, 2021Publication date: March 24, 2022Inventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN, Erik HERNANDEZ, Amber JOHNSON, Alexander BOULGAKOV, James L. BACHMAN, Helen SEIFERT
-
Publication number: 20210356473Abstract: Provided herein are rapid and reversible methods to non-specifically immobilize peptides and proteins irrespective of their sequence, as well as small molecules, on a solid support to allow for manipulations of and reactions with these molecules in a manner that does not require purification between steps, which increases sample yield and reduces the quantity of starting material required.Type: ApplicationFiled: October 4, 2019Publication date: November 18, 2021Inventors: Eric ANSLYN, Edward MARCOTTE, Cecil J. HOWARD, II, Jagannath SWAMINATHAN, Angela M. BARDO, Brendan M. FLOYD, James REUTHER
-
Patent number: 11162952Abstract: The disclosure concerns methods for identifying amino acids in peptides, including peptides including unnatural amino acids. Various aspects of the present disclosure provide compositions and methods for amino acid-type specific labeling, as well as methods for detecting the labels. Further disclosed herein are strategies for highly multiplexed, high-throughput peptide analysis.Type: GrantFiled: September 16, 2019Date of Patent: November 2, 2021Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan, Erik Hernandez, Amber Johnson, Alexander Boulgakov, James L. Bachman, Helen Seifert
-
Patent number: 11105812Abstract: The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: GrantFiled: March 16, 2017Date of Patent: August 31, 2021Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward Marcotte, Jagannath Swaminathan, Andrew Ellington, Eric V. Anslyn
-
Publication number: 20210215707Abstract: The present disclosure provides methods of identifying and quantifying the peptides displayed by the major histocompatibility complex (MHC). Such methods may comprise the ability to determine the type, identity, and quantity of each peptide displayed by the MHC. In some embodiments, these methods may be used to develop an anti-cancer therapy or type the HLA of a patient. Also provided herein are compositions comprising peptides from the MHC which have been prepared for sequencing.Type: ApplicationFiled: August 14, 2019Publication date: July 15, 2021Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward MARCOTTE, Eric ANSLYN, Alexander BOULGAKOV, Angela M. BARDO, Siyuan Stella WANG, Jagannath SWAMINATHAN, Fan TU
-
Publication number: 20210215706Abstract: The present disclosure provides methods of selectively label an amino acid residue on a peptide by replacing a post translational modification with a labeling moiety and sequencing the peptide to obtain the location of the amino acid residue and the identity of the post translational modification. In some aspects, the disclosure also provides methods of identifying the position, quantity, the identity of a post translational modification, or any combination thereof, in peptides which may be used for therapeutic purposes.Type: ApplicationFiled: January 22, 2021Publication date: July 15, 2021Applicant: Board of Regents, The University of Texas SystemInventors: Edward MARCOTTE, Eric ANSLYN, Jagannath SWAMINATHAN, Angela M. BARDO, Caroline M. HINSON, Cecil HOWARD, Brendan FLOYD
-
Publication number: 20210132076Abstract: The present disclosure provides methods for molecular neighborhood detection of molecules, such as by iterative proximity ligation or split-and-pool methods for obtaining positional information.Type: ApplicationFiled: January 11, 2021Publication date: May 6, 2021Applicant: Board of Regents, The University of Texas SystemInventors: Edward MARCOTTE, Jagannath SWAMINATHAN, Andrew ELLINGTON, Alexander BOULGAKOV, Jon LAURENT, Raghav SHROFF, Erhu XIONG, Sanchita BHADRA, Brendan FLOYD, Eric ANSLYN
-
Publication number: 20210072251Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with one or more unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: December 10, 2019Publication date: March 11, 2021Inventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN, Erik HERNANDEZ, Amber JOHNSON, Alexander BOULGAKOV, James L. BACHMAN, Helen SEIFERT
-
Publication number: 20210018511Abstract: The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: March 16, 2017Publication date: January 21, 2021Inventors: Edward Marcotte, Jagannath Swaminathan, Andrew Ellington, Eric Anslyn
-
Publication number: 20200124613Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with one or more unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: December 10, 2019Publication date: April 23, 2020Inventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN, Erik HERNANDEZ, Amber JOHNSON, Alexander BOULGAKOV, James L. BACHMAN, Helen SEIFERT
-
Patent number: 10545153Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with one or more unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: GrantFiled: September 15, 2015Date of Patent: January 28, 2020Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan, Erik Hernandez, Amber Johnson, Alexander Boulgakov, James L. Bachman, Helen Seifert
-
Publication number: 20200018768Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: September 16, 2019Publication date: January 16, 2020Inventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN, Erik HERNANDEZ, Amber JOHNSON, Alexander BOULGAKOV, James L. BACHMAN, Helen SEIFERT
-
Publication number: 20170276686Abstract: The present invention relates to the field of identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level. The present invention also relates to methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: September 15, 2015Publication date: September 28, 2017Inventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan, Erik Hernandez, Amber Johnson, Alexander Boulgakov, James L. Bachman, Helen Seifert