Abstract: An chimerical polypeptide that arrests proliferating cells in mitosis is provided. In general the polypeptide has an N-terminal transit peptide, such as HIV-1 Tat, and a C-terminal cell-cycle effector, such as a G2/M cyclin or a cytostatic factor. A polynucleotide under the control of a heterologous promoter that encodes a polypeptide that arrests cells in mitosis is also provided. The polynucleotide may, for example, encode a G2/M cyclin. Pharmaceutical compositions comprising the agent that inhibits transit through mitosis is provided. A method of treating patients suffering from a hyperplasia, such as cancer, psoriasis or benign prostate hyperplasia is provided.

Abstract: An chimerical polypeptide that arrests proliferating cells in mitosis is provided. In general the polypeptide has an N-terminal transit peptide, such as HIV-1 Tat, and a C-terminal cell-cycle effector, such as a G2/M cyclin or a cytostatic factor. A polynucleotide under the control of a heterologous promoter that encodes a polypeptide that arrests cells in mitosis is also provided. The polynucleotide may, for example, encode a G2/M cyclin. Pharmaceutical compositions comprising the agent that inhibits transit through mitosis is provided. A method of treating patients suffering from a hyperplasia, such as cancer, psoriasis or benign prostate hyperplasia is provided.

Abstract: Methods for deriving adult pluripotent stem cells from fully differentiated adult somatic cells by in vitro nuclear remodeling are provided. Cells cultured from a variety of tissue sources are treated in vitro to reverse the tissue specific epigenetic chromosomal changes associated with differentiation. Remodeled cells resemble embryonic stem cells by expressing telomerase and demonstrating pluripotency. The cells can be genetically modified to produce heterologous proteins or to correct for genetic defects. Methods for treating a human by implanting in vitro-derived adult pluripotent stem cells (“NUCREM™ cells”) and generating engineered tissues for implantation are also disclosed. Advantages to this invention include the non-use of embryos to obtain an unlimited supply of stem cells for therapy and the ability to generate autologous cells and tissues for therapeutic use.

Abstract: A new and improved apparatus is disclosed for reminding people to water a house plant. A user would hang a house plant from an eye hook located at the bottom of the bottom of the present invention, and an included readout would indicate when a user would need to water the house plant. The top half of the readout would be red and would not be visible when the houseplant would have sufficient water. The lower half of the readout would be green and would be visible through the bottom of the apparatus when the would have sufficient water. However, the top half of the readout would start to become visible through a transparent central portion of the apparatus when the houseplant would need to be watered. At the same time, the bottom half of the readout would be pulled into the apparatus and would gradually disappear as the need for water for the houseplant would increase.

Abstract: Methods for deriving adult pluripotent stem cells from fully differentiated adult somatic cells by in vitro nuclear remodeling are provided. Cells cultured from a variety of tissue sources are treated in vitro to reverse the tissue specific epigenetic chromosomal changes associated with differentiation. Remodeled cells resemble embryonic stem cells by expressing telomerase and demonstrating pluripotency. The cells can be genetically modified to produce heterologous proteins or to correct for genetic defects. Methods for treating a human by implanting in vitro-derived adult pluripotent stem cells (“NucREM™ cells”) and generating engineered tissues for implantation are also disclosed. Advantages to this invention include the non-use of embryos to obtain an unlimited supply of stem cells for therapy and the ability to generate autologous cells and tissues for therapeutic use.