Patents by Inventor Edwin Wei-Lung Lai

Edwin Wei-Lung Lai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
    Publication number: 20220226809
    Abstract: Methods of determining methylation of DNA are provided.
    Type: Application
    Filed: January 27, 2022
    Publication date: July 21, 2022
    Applicant: Cepheid
    Inventors: Edwin Wei-Lung Lai, Andrew Kohlway, Reuel Van Atta, Russell Higuchi, Alexander A. Gall, Kriszten Kocmond
  • Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
    Patent number: 11299726
    Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50° C. to about 100° C.; and recovering the nucleic acid from said lysis solution.
    Type: Grant
    Filed: January 23, 2019
    Date of Patent: April 12, 2022
    Assignee: CEPHEID
    Inventors: Edwin Wei-Lung Lai, Reuel Van Atta, Kenneth E. Ho
  • Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or mRNA expression levels in an automated reaction cartridge
    Patent number: 11260387
    Abstract: Methods of determining methylation of DNA are provided.
    Type: Grant
    Filed: December 12, 2017
    Date of Patent: March 1, 2022
    Assignee: CEPHEID
    Inventors: Edwin Wei-Lung Lai, Andrew Kohlway, Reuel Van Atta, Russell Higuchi, Alexander A. Gall, Kriszten Kocmond
  • Methods of Detecting Bladder Cancer
    Publication number: 20190382847
    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).
    Type: Application
    Filed: May 14, 2019
    Publication date: December 19, 2019
    Applicant: Cepheid
    Inventors: Leena McCann, Stacey Ellen Wallace, Edwin Wei-Lung Lai, Russell Higuchi
  • Methods of Detecting Bladder Cancer
    Publication number: 20190360050
    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
    Type: Application
    Filed: February 11, 2019
    Publication date: November 28, 2019
    Inventors: Russell Higuchi, Stacey Ellen Wallace, Edwin Wei-Lung Lai
  • METHODS FOR DNA AND RNA EXTRACTION FROM FIXED PARAFFIN-EMBEDDED TISSUE SAMPLES
    Publication number: 20190225959
    Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50° C. to about 100° C.; and recovering the nucleic acid from said lysis solution.
    Type: Application
    Filed: January 23, 2019
    Publication date: July 25, 2019
    Inventors: Edwin Wei-Lung Lai, Reuel Van Atta, Kenneth E. Ho
  • Methods of detecting bladder cancer
    Patent number: 10329622
    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).
    Type: Grant
    Filed: March 6, 2014
    Date of Patent: June 25, 2019
    Assignee: Cepheid
    Inventors: Leena McCann, Stacey Ellen Wallace, Edwin Wei-Lung Lai, Russell Higuchi
  • Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
    Patent number: 10233440
    Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 C to about 100 C; and recovering the nucleic acid from said lysis solution.
    Type: Grant
    Filed: September 26, 2013
    Date of Patent: March 19, 2019
    Assignee: CEPHEID
    Inventors: Edwin Wei-Lung Lai, Reuel Van Atta, Kenneth E. Ho
  • Compositions and methods for detecting markers associated with bladder cancer
    Patent number: 10202653
    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: February 12, 2019
    Assignee: Cepheid
    Inventors: Russell Higuchi, Stacey Ellen Wallace, Edwin Wei-Lung Lai
  • INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
    Publication number: 20180214864
    Abstract: Methods of determining methylation of DNA are provided.
    Type: Application
    Filed: December 12, 2017
    Publication date: August 2, 2018
    Inventors: EDWIN WEI-LUNG LAI, ANDREW KOHLWAY, REUEL VAN ATTA, RUSSELL HIGUCHI, ALEXANDER A. GALL, KRISZTEN KOCMOND
  • TWO-PRIMER PCR FOR MICRORNA MULTIPLEX ASSAY
    Publication number: 20150322510
    Abstract: The present invention provides method for amplifying a specific RNA molecule in a sample, the method comprising: (a) adding a poly(ribonucleotide) sequence to RNA molecules in the sample; (b) reverse transcribing the poly-adenylated RNA molecules using a reverse primer comprising a sequence that anneals to said poly(ribonucleotide) sequence; and (c) amplifying and detecting the cDNA molecule(s) using the same reverse primer and using a forward primer specific for the RNA molecule to be detected; wherein at least one of the forward and reverse primers comprises a hairpin primer. The invention also provides kits useful for practicing this method.
    Type: Application
    Filed: September 25, 2013
    Publication date: November 12, 2015
    Applicant: CEPHEID
    Inventors: Russell HIGUCHI, Edwin Wei-Lung LAI, Sergey LOKHOV
  • METHODS FOR DNA AND RNA EXTRACTION FROM FIXED PARAFFIN-EMBEDDED TISSUE SAMPLES
    Publication number: 20150252354
    Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 C to about 100 C; and recovering the nucleic acid from said lysis solution.
    Type: Application
    Filed: September 26, 2013
    Publication date: September 10, 2015
    Inventors: Edwin Wei-Lung Lai, Reuel Vanatta, Kenneth E. Ho
  • METHODS OF DETECTING BLADDER CANCER
    Publication number: 20150133325
    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
    Type: Application
    Filed: April 19, 2013
    Publication date: May 14, 2015
    Inventors: Russell Higuchi, Stacey Ellen Wallace, Edwin Wei-Lung Lai
  • METHODS OF DETECTING BLADDER CANCER
    Publication number: 20140309127
    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of detecting low grade bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting androgen receptor (AR) and/or uroplakin 1B (UPK1B).
    Type: Application
    Filed: March 6, 2014
    Publication date: October 16, 2014
    Applicant: Cepheid
    Inventors: Leena McCann, Stacey Ellen Wallace, Edwin Wei-Lung Lai, Russell Higuchi