Patents by Inventor Edyta Krzymanska-Olejnik

Edyta Krzymanska-Olejnik has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20180274004
    Abstract: Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe is Listeria.
    Type: Application
    Filed: January 10, 2018
    Publication date: September 27, 2018
    Inventors: Michael Sandor Koeris, Michael Cappillino, Edyta Krzymanska-Olejnik, Daniel Robert Brownell, Robert Patrick Shivers, Jayson L. Bowers, Timothy Kuan-Ta Lu
  • Patent number: 10039795
    Abstract: Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is Listeria.
    Type: Grant
    Filed: May 8, 2015
    Date of Patent: August 7, 2018
    Assignee: Institute for Environmental Health, Inc.
    Inventors: Michael Sandor Koeris, Jayson L. Bowers, Daniel Robert Brownell, Edyta Krzymanska-Olejnik, Robert Patrick Shivers, Michael Cappillino
  • Patent number: 9828625
    Abstract: Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: November 28, 2017
    Assignee: Phage Diagnostics, Inc.
    Inventors: Michael Sandor Koeris, Julie Thomason, Michael Cappillino, Robert Patrick Shivers, Daniel Robert Brownell, Jayson L. Bowers, Timothy Kuan Ta Lu, Edyta Krzymanska-Olejnik
  • Publication number: 20150344930
    Abstract: Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is Listeria.
    Type: Application
    Filed: May 8, 2015
    Publication date: December 3, 2015
    Inventors: Michael Sandor KOERIS, Jayson L. BOWERS, Daniel Robert BROWNELL, Edyta KRZYMANSKA-OLEJNIK, Robert Patrick SHIVERS, Michael CAPPILLINO
  • Publication number: 20150004595
    Abstract: Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
    Type: Application
    Filed: June 19, 2014
    Publication date: January 1, 2015
    Inventors: Michael Sandor KOERIS, Julie THOMASON, Michael CAPPILLINO, Robert Patrick SHIVERS, Daniel Robert BROWNELL, Jayson L. BOWERS, Timothy Kuan Ta LU, Edyta KRZYMANSKA-OLEJNIK
  • Publication number: 20120040340
    Abstract: The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.
    Type: Application
    Filed: October 27, 2011
    Publication date: February 16, 2012
    Applicant: HELICOS BIOSCIENCES CORPORATION
    Inventors: J. William Efcavitch, Suhaib Siddiqi, Philip R. Buzby, Judith Mitchell, Edyta Krzymanska-Olejnik, Subramanian Marappan, Xiaopeng Bai, Atanu Roy, Mirna Jarosz, Jayson Bowers
  • Patent number: 8114973
    Abstract: The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.
    Type: Grant
    Filed: October 2, 2008
    Date of Patent: February 14, 2012
    Assignee: Helicos Biosciences Corporation
    Inventors: Suhaib Siddiqi, Subramanian Marappan, Judith Mitchell, Edyta Krzymanska-Olejnik, Atanu Roy, Xiaopeng Bai
  • Patent number: 8071755
    Abstract: The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: December 6, 2011
    Assignee: Helicos Biosciences Corporation
    Inventors: J. William Efcavitch, Suhaib Siddiqi, Philip R. Buzby, Judith Mitchell, Edyta Krzymanska-Olejnik, Subramanian Marappan, Xiaopeng Bai, Atanu Roy, Mirna Jarosz, Jayson Bowers
  • Publication number: 20090263868
    Abstract: Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable marker nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.
    Type: Application
    Filed: June 16, 2009
    Publication date: October 22, 2009
    Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Kenneth J. Rothschild
  • Publication number: 20090186771
    Abstract: The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.
    Type: Application
    Filed: January 15, 2009
    Publication date: July 23, 2009
    Applicant: Helicos BioSciences Corporation
    Inventors: Suhaib M. Siddiqi, Edyta Krzymanska-Olejnik, Herman Antonio Orgueira, Xiaopeng Bai
  • Publication number: 20090186351
    Abstract: The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.
    Type: Application
    Filed: October 2, 2008
    Publication date: July 23, 2009
    Applicant: HELICOS BIOSCIENCES CORPORATION
    Inventors: Suhaib Siddiqi, Subramanian Marappan, Judith Mitchell, Edyta Krzymanska-Olejnik, Atanu Roy, Xiaopeng Bai
  • Patent number: 7547530
    Abstract: Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.
    Type: Grant
    Filed: February 9, 2006
    Date of Patent: June 16, 2009
    Assignee: AmberGen, Inc.
    Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Kenneth J. Rothschild
  • Patent number: 7524941
    Abstract: The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.
    Type: Grant
    Filed: September 11, 2007
    Date of Patent: April 28, 2009
    Assignee: Ambergen, Inc.
    Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Sergey Mamaev, Kenneth J. Rothschild
  • Publication number: 20090061437
    Abstract: The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.
    Type: Application
    Filed: April 4, 2008
    Publication date: March 5, 2009
    Applicant: HELICOS BIOSCIENCES CORPORATION
    Inventors: J. William Efcavitch, Suhaib Siddiqi, Philip R. Buzby, Judith Mitchell, Edyta Krzymanska-Olejnik, Subramanian Marappan, Xiaopeng Bai, Atanu Roy, Mirna Jarosz, Jayson Bowers
  • Publication number: 20080287710
    Abstract: The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.
    Type: Application
    Filed: September 11, 2007
    Publication date: November 20, 2008
    Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Sergey Mamaev, Kenneth J. Rothschild
  • Publication number: 20080103053
    Abstract: The invention provides a family of tethered nucleotide analogs useful in sequencing nucleic acids containing a homopolymer region comprising, for example, two or more base repeats, and to sequencing methods using such tethered nucleotide analogs.
    Type: Application
    Filed: May 14, 2007
    Publication date: May 1, 2008
    Applicant: Helicos BioSciences Corporation
    Inventors: Suhaib Siddiqi, Hernan Orgueira, Edyta Krzymanska-Olejnik, Subramanian Marappan, Philip R. Buzby, Atanu Roy
  • Publication number: 20080026380
    Abstract: The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.
    Type: Application
    Filed: July 31, 2006
    Publication date: January 31, 2008
    Inventors: Suhaib M. Siddiqi, Edyta Krzymanska-Olejnik, Herman Antonio Orgueira, Xiaopeng Bai
  • Publication number: 20080026381
    Abstract: The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.
    Type: Application
    Filed: July 31, 2006
    Publication date: January 31, 2008
    Inventors: Suhaib M. Siddiqi, Edyta Krzymanska-Olejnik, Herman Antonio Orgueira, Xiaopeng Bai
  • Publication number: 20080026379
    Abstract: The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.
    Type: Application
    Filed: July 31, 2006
    Publication date: January 31, 2008
    Inventors: Suhaib M. Siddiqi, Edyta Krzymanska-Olejnik, Herman Antonio Orgueira, Xiaopeng Bai
  • Patent number: 7288372
    Abstract: The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.
    Type: Grant
    Filed: January 16, 2003
    Date of Patent: October 30, 2007
    Assignee: Ambergen, Inc.
    Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Sergey Mamaev, Kenneth J. Rothschild