Patents by Inventor Eevi LIPPONEN

Eevi LIPPONEN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • LARGE SCALE PEI-MEDIATED PLASMID TRANSFECTION
    Publication number: 20240247218
    Abstract: We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
    Type: Application
    Filed: September 1, 2023
    Publication date: July 25, 2024
    Inventors: Hanna P. Lesch, Joonas Malinen, Eevi Lipponen, Anniina Valkama, Hanna Leinonen
  • Large-scale PEI-mediated plasmid transfection
    Patent number: 11781102
    Abstract: We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: October 10, 2023
    Assignee: Trizell Ltd.
    Inventors: Hanna P. Lesch, Joonas Malinen, Eevi Lipponen, Anniina Valkama, Hanna Leinonen
  • Large-Scale PEI-Mediated Plasmid Transfection
    Publication number: 20200165557
    Abstract: We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
    Type: Application
    Filed: April 24, 2017
    Publication date: May 28, 2020
    Applicant: Trizell Ltd.
    Inventors: Hanna P. LESCH, Joonas MALINEN, Eevi LIPPONEN, Anniina VALKAMA, Hanna LEINONEN
  • Seeding An Adherent Cell Bioreactor With Non-Adherent Cells Increases Seeding Density Limit And Reduces Required Expansion Time
    Publication number: 20190276846
    Abstract: We have found a counter-intuitive way to improve the commercial-scale production of recombinant biological products in adherent-cell bioreactors, which reduces the risk of cell culture contamination, increases total yield and reduces the delay between seeding and harvest, thus minimizing expression product degradation, by inter alia inoculating an adherent culture bioreactor with suspension-adapted producer cells.
    Type: Application
    Filed: March 12, 2019
    Publication date: September 12, 2019
    Applicant: Trizell Ltd.
    Inventors: Eevi LIPPONEN, Hanna P. LESCH, Minna KARHINEN, Robert SHAW, Seppo YLA-HERTTUALA, Joonas MALINEN, Nigel PARKER
  • Seeding An Adherent Cell Bioreactor With Non-Adherent Cells Increases Seeding Density Limit And Reduces Required Expansion Time
    Publication number: 20170051309
    Abstract: We have found a counter-intuitive way to improve the commercial-scale production of recombinant biological products in adherent-cell bioreactors, which reduces the risk of cell culture contamination, increases total yield and reduces the delay between seeding and harvest, thus minimizing expression product degradation, by inter alia inoculating an adherent culture bioreactor with suspension-adapted producer cells
    Type: Application
    Filed: August 26, 2015
    Publication date: February 23, 2017
    Applicant: Trizell Ltd.
    Inventors: Hanna P. LESCH, Nigel PARKER, Minna KARHINEN, Robert SHAW, Seppo YLA-HERTTUALA, Jonas MALINEN, Eevi LIPPONEN