Abstract: The invention relates to the purification of bispecific antibodies carrying a different specificity for each binding site of the immunoglobulin molecule from a mixture of monospecific antibodies. The bispecific antibodies are composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other a Lambda constant domain. This invention in particular relates to the isolation of these bispecific antibodies from mixtures that contain monospecific antibodies having two Kappa light chains or portions thereof and monospecific antibodies having two Lambda light chains or portions thereof. The invention also provides the methods of efficiently purifying these bispecific antibodies.

Abstract: The invention relates to a process for the production of chromatography columns or capillaries containing sorbents of monolithic mouldings which can remain directly in their gelation mould after production. This is achieved by the process according to the invention, in which the gelation mould is repeatedly filled with the monomer sol.

Abstract: The invention relates to polyamides which are derivatized with polymerizable double bonds and are obtainable by reaction of the amino groups of the polyamide with an amino-reactive compound which contains a polymerizable double bond, or by reaction of the carboxyl groups of the polyamide with a carboxyl group-reactive compound which contains a polymerizable double bond. Examples of amino-reactive compounds which contain a polymerizable double bond are the anhydrides of ethylenically unsaturated carboxylic acids, such as acrylic acid chloride or anhydride, methacrylic acid chloride or anhydride, or vinylazlactone derivatives. An example of a carboxyl group-reactive compound is allylamine.These derivatized polyamides can be converted into polymer-modified base polymers (typically block polymers) with improved properties. These polymer-modified base polymers are suitable as adsorbents for chromatography and as carriers for enzymes.