Abstract: Systems, devices and methods for integrating world-to-chip interfaces with digital microfluidics for bacterial transformation and enzymatic assays are described herein. The devices include a microfluidic device having a first plate comprising a cell culture region for maintaining a cell culture and a reservoir for storing reagents to induce at least a portion of the cell culture or to mix with other reagents and a second plate spaced apart from the first plate, the second plate defining one or more openings extending through the second plate. The device may include a reagent well coupled to the second plate. The reagent well may be configured to refill the reservoir on the first plate with liquid reagent via the one or more openings of the second plate. The device may also include a thermoelectric module (TEM) coupled to the first plate or the second plate for managing temperature control of the device.