Abstract: A problem to be solved is to perform an immunoassay method for BG in a biological sample having a sensitivity equivalent to and a reactivity similar to a Limulus amebocyte lysate reagent. The problem can be solved by an immunoassay method for ?-D-glucan in a biological sample, comprising assaying ?-D-glucan in the biological sample by using a monoclonal antibody binding to (1?3)-?-D-glucan having a degree of polymerization of 4.

Abstract: A problem to be solved is to perform an immunoassay method for BG in a biological sample simply operated and having a sensitivity equivalent to that of a Limulus reagent. The problem can be solved by combining a pretreatment of a biological samples with an alkaline solution and a use of anti-BG monoclonal antibody specifically reacting with BG.

Abstract: Provided is a method for separating and detecting various hemoglobins including abnormal hemoglobins and thalassemia markers. A method for analyzing hemoglobins, comprising separating hemoglobins in a sample by cation exchange chromatography, wherein an eluent having a pH of 8.1 or more and an osmotic pressure of 40 mOsm/kg or less is used for separation of the hemoglobins.

Abstract: It is intended to provide highly sensitive and specific, rapid, and convenient method for evaluating a risk of hepatocellular carcinoma. The method for evaluating a risk of hepatocellular carcinoma comprises: (1) amplifying bisulfite-treated DNA derived from a liver tissue of a subject, wherein the DNA comprises a CpG site of an exon region of MGRN1 gene; (2) subjecting the obtained amplification product to ion exchange chromatography; and (3) determining whether or not the DNA is DNA obtained from a subject having a high risk of development of hepatocellular carcinoma on the basis of a peak shape of a detection signal of the chromatography.