Abstract: Provide is a braided wire manufacturing method and a braided wire manufacturing apparatus which can produce a braided wire accurately braided without distortion. In a braided wire manufacturing apparatus, a plurality of conductive wires fed from a wire feeding mechanism are braided into a braided wire from a convergence position after passing through a mesh hole die, and the braided wire after passing along a guide roller is wound up using a capstan unit. The guide roller has a guide width for horizontal direction restriction so that movement of the braided wire in the horizontal direction falls within a predetermined restriction width from an ideal center position. The guide roller is provided at a height of a braid pitch of the braided wire in the perpendicular direction from the convergence position as a second feature.

Abstract: A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3? end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3? end is DNA, comprising: (a) adding polydeoxyadenosine to the 3? DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5? side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b).

Abstract: Disclosed is a method for obtaining differentiated cells and/or differentiated cell products, including the steps of: introducing undifferentiated cells into a perfused organ or living tissue; subjecting the undifferentiated cells introduced to perfusion culture together with the organ or living tissue so as to allow the undifferentiated cells to differentiate, thereby obtaining differentiated cells and/or differentiated cell products; collecting a perfusion culture solution that contains the resulting differentiated cells and/or differentiated cell products; and obtaining the differentiated cells and/or differentiated cell products contained in the collected perfusion culture solution.

Abstract: Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v) %.

Abstract: An object is to provide a TCR closing system that enables not only bias-free analysis of TCR repertoires, but also collection of antigen-specific TCR ?/? cDNA pairs and evaluation of functions thereof. There is provided a method for producing a gene of T cell receptor (TCR) specific to an antigen A, which comprises 1) the step of stimulating a group of T cells including a T cell specific to an antigen A or one T cell specific to an antigen A under a condition effective for amplification of a TCR gene; 2) the step of identifying a T cell specific to an antigen A among the group of T cells including a T cell specific to the antigen A, and sorting one T cell specific to the antigen A into a vessel; and 3) the step of subjecting the one activated T cell specific to the antigen A in the vessel to PCR to amplify a gene of TCR specific to the antigen A.

Abstract: Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v) %.

Abstract: A mammalian cell suspension prevents pulmonary embolism formation during administration of mammalian cells, such as mammalian stem cells, through a blood vessel, and a preventive agent against pulmonary embolism formation during administration of mammalian cells through a blood vessel. suspending mammalian cells, such as mammalian stem cells, are suspended in a physiological aqueous solution containing trehalose or its derivative, or a salt thereof as an active ingredient to prepare a mammalian cell suspension for preventing pulmonary embolism formation during administration of the mammalian cells through a blood vessel, including the mammalian cells and trehalose or its derivative, or a salt thereof as active ingredients. Examples of the mammalian cells can include pancreatic islet cells, dendritic cells, natural killer cells, alpha/beta T cells, gamma/delta T cells, and cytotoxic T lymphocytes in addition to mammalian stem cells.

Abstract: The present invention provides an artificial kidney precursor containing a non-human mammalian metanephros separated out from a living body, wherein the metanephros has been subjected to freezing and thawing treatments outside a living body, and contains mammalian mesenchymal stem cells transferred outside a living body, and a method of production thereof.

Abstract: Provide is a braided wire manufacturing method and a braided wire manufacturing apparatus which can produce a braided wire accurately braided without distortion. In a braided wire manufacturing apparatus, a plurality of conductive wires fed from a wire feeding mechanism are braided into a braided wire from a convergence position after passing through a mesh hole die, and the braided wire after passing along a guide roller is wound up using a capstan unit. The guide roller has a guide width for horizontal direction restriction so that movement of the braided wire in the horizontal direction falls within a predetermined restriction width from an ideal center position. The guide roller is provided at a height of a braid pitch of the braided wire in the perpendicular direction from the convergence position as a second feature.

Abstract: An object of the present invention is to stimulate a T cell without using a peptide/MHC tetramer. In the present invention, the step of supplying an antigen peptide to a T cell having a T cell receptor (TCR) that can recognize the antigen peptide on cell surface to form a complex of a major histocompatibility complex (MHC) molecule on the cell surface of the T cell and the antigen peptide is used, and the T cell is stimulated through recognition by TCR of the antigen peptide as the MHC molecule-antigen peptide complex on the cell surface of the same T cell. Such a stimulating and activating method would be applicable to not only T cells, but also various cells. According to the present invention, an antigen-specific T cell can be identified without establishing any antigen-specific T cell strain, and without using such a reagent as MHC/peptide tetramer. That is, a cancer-specific T cell can be efficiently and conveniently identified.

Abstract: A capstan device for taking up a braided wire in a braided wire manufacturing apparatus for braiding a plurality of wires to manufacture the braided wire. The capstan device includes a capstan roller having a tapered outer peripheral surface and provided with a flange portion on an end at a small diameter side thereof, and a guide member. The guide member has a first guide surface provided to be turned toward a large diameter side of the capstan roller in an axial direction of the capstan roller in a position on a larger diameter side than a portion having the smallest diameter in the tapered outer peripheral surface, and a second guide surface provided to be protruded from the first guide surface at an outer peripheral side of the tapered outer peripheral surface.

Abstract: The present invention provides a method for evaluating preservative effect of a tissue preservation solution, comprising immersing a mammalian tissue introduced with a luminescence or fluorescence labeling gene in the tissue preservation solution, measuring a luminescence or fluorescence level by the labeling gene in the tissue after immersion, and evaluating the preservative effect of the tissue preservation solution based on the luminescence or fluorescence level.

Abstract: A softening apparatus includes a cooling liquid reservoir for holding a cooling liquid, a first conducting sheave provided outside of the cooling liquid reservoir for applying a voltage to a wire while guiding the wire, and a second conducting sheave provided in the cooling liquid reservoir for letting a current flow through the wire while guiding the wire as the wire is fed via the first conducting sheave. The softening apparatus is also provided with a liquid level detector for detecting a liquid level of the cooling liquid in the cooling liquid reservoir. A heating path length of the wire between the first conducting sheave and the second conducting sheave is controlled based on a result of detection by the liquid level detector.

Abstract: An all-solid-state cell contains a combination of an electrode active material and a solid electrolyte, and has a plate-shaped fired solid electrolyte body of a ceramic containing a solid electrolyte, a first electrode layer (e.g. a positive electrode) integrally formed on one surface of the fired solid electrolyte body by mixing and firing an electrode active material and a solid electrolyte, and a second electrode layer (e.g. a negative electrode) integrally formed on the other surface of the fired solid electrolyte body by mixing and firing an electrode active material and a solid electrolyte. The solid electrolyte materials added to the first electrode layer and the second electrode layer comprise an amorphous polyanion compound.

Abstract: An all-solid-state cell has a fired solid electrolyte body, a first electrode layer integrally formed on one surface of the fired solid electrolyte body by mixing and firing an electrode active material and a solid electrolyte, and a second electrode layer integrally formed on the other surface of the fired solid electrolyte body by mixing and firing an electrode active material and a solid electrolyte. The first and the second electrode layers are formed by mixing and firing the electrode active material and the amorphous solid electrolyte, which satisfy the relation Ty>Tz (wherein Ty is a temperature at which the capacity of the electrode active material is lowered by reaction between the electrode active material and the solid electrolyte material, and Tz is a temperature at which the solid electrolyte material is shrunk by firing).

Abstract: The purpose of the invention is to provide means with which it is possible to efficiently select a vector to which a foreign gene has been introduced when a foreign gene is to be introduced by homologous recombination to a vector having multiple sequences homologous with one another. The vector comprises, in succession, a replication origin, a sequence A, a marker gene X, two sequences C and D for introducing a foreign gene by homologous recombination, and a sequence B homologous with sequence A. The two sequences C and D are directly or indirectly adjacent to one another. The vector is used for introducing a foreign gene between the two adjacent sequences C and D.

Abstract: A multi unit controller has a plurality of base units each of which includes a control unit connector, a base unit connector through which the base units juxtaposed to each other are connected to each other, and a plurality of control units connected to the base units respectively through the respective control unit connector, wherein the base units juxtaposed vertically.

Abstract: Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v)%.

Abstract: A processing unit is connected to another processing unit through a system bus composed of serial signal communication line and synchronization signal communication line to be able to communicate therewith. When an operation unit detects abnormal state in the processing unit, the operation unit supplies notification of detection of the abnormal state to synchronization unit. The synchronization unit transmits the received detection notification of abnormal state to the other processing unit through the synchronization signal communication line. Conversion unit receives parallel communication data from the operation unit through important signal line instead of general signal line and converts the received parallel signal into serial signal to be transmitted to the other processing unit through the serial signal communication line, thereby soundness among processing units connected to the system bus is ensured when the system bus is configured to attain serial communication.

Abstract: A laser oscillator control device includes a controller having a transmitter section and a receiver section; a laser oscillator having a transmitter section and a receiver section and communicating with the controller via a communication line; wherein the laser oscillator control device outputs a control signal from the controller to the laser oscillator, based on a status signal indicating operational state of the laser oscillator sent from the laser oscillator to the controller. The controller has an alternating signal transmitter circuit that generates an alternating signal that changes at a predetermined period, and sends this alternating signal to the laser oscillator, and the laser oscillator has a return signal transmitter circuit which generates a return signal that changes periodically in correspondence to the alternating signal from the controller, and sends this return signal to the controller.