Abstract: A method for the determination and correction of background signals in a spectrum, consisting of signals of a plurality of spectral points, characterized by the steps of: Calculating at least three statistic or analytic functions of the signal values of the spectrum, attributing probabilities Pi(band) for the presence of bands to each point in each of the calculated functions: Adding the probabilities Pi(band) up to an overall probability ?Pi(band) from all calculated functions for each point; calculating a probability P(background) for the presence of background for each point in the spectrum from said overall probability ?Pi(band) according to P(background)=1??Pi(band) wherein negative values are set to zero; and calculating a fit of the signal values at all points of the original spectrum wherein the signal in each point is taken into account in the fit only with the respective probability for the presence of background P(background), and subtraction of the background function determined in such a way from

Abstract: A method for the spectral analysis of samples in a graphite tube, comprising the steps of: inserting a liquid sample into a graphite tube; drying the sample by heating the graphite tube; transferring the sample into a particle cloud by further heating up the graphite tube; and measuring one of the optical signals influenced or generated by the sample with a detector; wherein image sequences of the interior of the graphite tube are recorded with a two-dimensional camera having a plurality of image elements over selected periods of time during the spectral analysis; is characterized in that the images of the image sequences are automatically processed with image processing methods, wherein a reference image of the interior of the graphite tube is determined; and the condition of the graphite tube, of the sample and/or of a dosing means for inserting the sample into the graphite tube is determined by comparison of the images of the image sequences to the reference image.

Abstract: A method for the spectral analysis of samples in a graphite tube, comprising the steps of: inserting a liquid sample into a graphite tube; drying the sample by heating the graphite tube; transferring the sample into a particle cloud by further heating up the graphite tube; and measuring one of the optical signals influenced or generated by the sample with a detector; wherein image sequences of the interior of the graphite tube are recorded with a two-dimensional camera having a plurality of image elements over selected periods of time during the spectral analysis; is characterized in that the images of the image sequences are automatically processed with image processing methods, wherein a reference image of the interior of the graphite tube is determined; and the condition of the graphite tube, of the sample and/or of a dosing means for inserting the sample into the graphite tube is determined by comparison of the images of the image sequences to the reference image.

Abstract: A method for the determination and correction of background signals in a spectrum, consisting of signals of a plurality of spectral points, characterized by the steps of: Calculating at least three statistic or analytic functions of the signal values of the spectrum, attributing probabilities Pi(band) for the presence of bands to each point in each of the calculated functions: Adding the probabilities Pi(band) up to an overall probability ?Pi(band) from all calculated functions for each point; calculating a probability P(background) for the presence of background for each point in the spectrum from said overall probability ?Pi(band) according to P(background)=1??Pi(band) wherein negative values are set to zero; and calculating a fit of the signal values at all points of the original spectrum wherein the signal in each point is taken into account in the fit only with the respective probability for the presence of background P(background), and subtraction of the background function determined in such a way from

Abstract: The invention is directed to a method for the spectrometric determination of the oxygen saturation of blood in the presence of optical disturbance variables in which transmission measurements and reflection measurements are carried out in at least two wavelengths that are isosbestic for hemoglobin and oxyhemoglobin, and at least one other wavelength at which the extinction of hemoglobin and oxyhemoglobin differ. Corresponding auxiliary functions are defined in the measurement spectrum (M) and in the reference spectra of hemoglobin and oxyhemoglobin, at least two of the measurement values or two of the reference values for the isosbestic wavelengths lying on this auxiliary function. A corrected measurement spectrum (M?) is generated by means of the two auxiliary functions. The oxygen saturation is determined by comparing the changed data of this corrected measurement spectrum (M?) with the data of the reference spectra at the other wavelength.

Abstract: The invention is directed to a method for the spectrometric determination of the oxygen saturation of blood in the presence of optical disturbance variables in which transmission measurements and reflection measurements are carried out in at least two wavelengths that are isosbestic for hemoglobin and oxyhemoglobin, and at least one other wavelength at which the extinction of hemoglobin and oxyhemoglobin differ. Corresponding auxiliary functions are defined in the measurement spectrum (M) and in the reference spectra of hemoglobin and oxyhemoglobin, at least two of the measurement values or two of the reference values for the isosbestic wavelengths lying on this auxiliary function. A corrected measurement spectrum (M?) is generated by the two auxiliary functions. The oxygen saturation is determined by comparing the changed data of this corrected measurement spectrum (M?) with the data of the reference spectra at the other wavelength.

Abstract: The invention is directed to a method for the spectrometric determination of the oxygen saturation of blood in the presence of optical disturbance variables in which transmission measurements and reflection measurements are carried out in at least two wavelengths that are isosbestic for hemoglobin and oxyhemoglobin, and at least one other wavelength at which the extinction of hemoglobin and oxyhemoglobin differ. Corresponding auxiliary functions are defined in the measurement spectrum (M) and in the reference spectra of hemoglobin and oxyhemoglobin, at least two of the measurement values or two of the reference values for the isosbestic wavelengths lying on this auxiliary function. A corrected measurement spectrum (M?) is generated by the two auxiliary functions. The oxygen saturation is determined by comparing the changed data of this corrected measurement spectrum (M?) with the data of the reference spectra at the other wavelength.

Abstract: A method and apparatus for determining fluorophores on objects, especially on the living ocular fundus, which makes it possible to reliably distinguish at least partially overlapping fluorophores of objects in excitation and/or fluorescence spectra even if fluorescence intensities are very low and to select them for analysis, and optionally create a two-dimensional representation. The object (4), e.g., the ocular fundus for ophthalmological examinations, is illuminated point to point with a pulsed laser (1) and excited to autofluorescence with two-dimensional extension. The transient fluorescence light created after excitation by each laser pulse is detected in time-correlated individual photon counting (11). From the time behavior of the fluorescence light determined by individual photon counting for each site of autofluorescence, the fluorescence decay time constants are calculated, and conclusions are drawn therefrom regarding the excited fluorophores in the object (4).