Abstract: Methods of evaluating a cellular sample for HER-2/neu expression are provided. Aspects of the methods include flow cytometrically obtaining fluorescence emission data from a cellular sample fluorescently labelled HER-2/neu specific binding member and employing the sample fluorescence emission data with standard fluorescence emission data to obtain a value of fluorescently labelled HER-2/neu specific binding members bound per cell.

Abstract: Provided are methods of amplifying nucleic acids. The methods include combining a nucleic acid sample, a known amount of one or more competitive internal standard nucleic acids, and one or more amplification primers adapted to amplify one or more nucleic acids of interest present in the nucleic acid sample and the one or more competitive internal standard nucleic acids. The nucleic acid sample, competitive internal standard nucleic acids, and amplification primers are combined in a reaction mixture under conditions sufficient to amplify the one or more nucleic acids of interest and the one or more competitive internal standard nucleic acids. Aspects of the present disclosure further include compositions and kits that find use in practicing embodiments of the methods.

Abstract: The present invention relates to methods of screening or isolating stable mutant binding proteins from a protein library of mutant proteins. The methods comprise culturing host cells under conditions suitable for protein expression. The collection of host cells comprise collectively a set of expression vectors, and this collection of expression vectors encodes the various members of a protein library of mutant binding proteins. The cultured cells, harboring the expression vectors, are contacted with a ligand, and differences in interaction, among the individual host cells, with the ligand are detected. The individual host cells displaying the desired interaction with the ligand are then separated from individual host cells that do not display the desired interaction with the ligand.