Abstract: This invention relates to the inhibition and down-regulation of survivin expression. The invention provides methods and antisense oligonucleotides for inhibiting or down-regulating survivin expression in cells and promoting apoptosis and cell necrosis.

Abstract: Disclosed is a composition comprising at least two synthetic, cooperative oligonucleotides, each comprising a region complementary to one of tandem, non-overlapping regions of a target single-stranded nucleic acid, and each further comprising a non-nucleotidic binding partner at a terminus of each of the oligonucleotides, such that the binding partners can interact with each other to form a stable complex. Also disclosed are dimeric structures, ternary complexes, pharmaceutical formulations, and methods utilizing the cooperative oligonucleotides of the invention.

Abstract: Disclosed are synthetic oligonucleotides complementary to nucleic acids encoding epidermal growth factor and methods of their use.

Abstract: The invention relates to the therapeutic use of oligonucleotides or oligonucleotide analogs as immunostimulatory agents in immunotherapy applications. The invention provides methods for enhancing the immune response caused by immunostimulatory oligonucleotide compounds.

Abstract: The present invention comprises a new class of oligonucleobases (e.g., oligonucleotides), which we call “pseudo-cyclic oligonucleotides” (PCOs). PCOs contain two oligonucleotide segments attached through their 3′-3′ or 5′-5′ ends. One of the segments (the “functional segment”) of the PCO has some functionality (e.g., an antisense oligonucleotide complementary to a target mRNA). Another segment (the “protective segment”) is complementary to the 3′- or 5′-terminal end of the functional segment (depending on the end through which it is attached to the functional segment). As a result of complementarity between the functional and protective segment segments, PCOs form intramolecular pseudo-cyclic structures in the absence of the target nucleic acids (e.g., RNA).

Abstract: Disclosed is a composition comprising at least two synthetic, cooperative oligonucleotides, each comprising a region complementary to one of tandem, non-overlapping regions of a target single-stranded nucleic acid, and each further comprising a dimerization domain at a terminus of each of the oligonucleotides, the dimerization domains of the oligonucleotides being complementary to each other. Also disclosed are duplex structures, ternary complexes, pharmaceutical formulations, and methods utilizing the cooperative oligonucleotides of the invention.

Abstract: The present invention provides novel compounds and methods for purifying oligonucleotides. The compounds according to the invention are multimeric oligonucleotides comprising a multimerization domain for inducing multimeric oligonucleotide aggregation, a hybridization domain that is complementary to a target oligonucleotide whose isolation is desired, and a linker domain connecting the multimerization domain and the hybridization domain. Other compounds of the invention comprise dendrimers having oligonucleotides with hybridization domains linked thereto.The methods of the invention comprise contacting the compounds of the invention with a solution containing a target oligonucleotide whose purification is desired. The target oligonucleotide hybridizes to the hybridization domain of the inventive compounds, thereby forming an aggregate. Synthetic failure sequences (N-1, N-2, etc.

Abstract: The present invention provides a novel class of oligonucleotides useful for antisense purposes. The oligonucleotides of the invention comprise both deoxyribonucleotides with "natural" 3'-5' internucleotide linkages and ribonucleotides with 2'-5' internucleotide linkages. Because of their conformation structure, oligonucleotides according to the invention possess uniform intra-phosphate distances throughout the oligonucleotide chain, allowing them to bind efficiently to complementary DNA and RNA with "natural" 3'-5' internucleotide linkages. The oligonucleotides according to the invention advantageously exhibit diminished immune stimulation and significantly reduced effect on both complement and coagulation as compared to 3'-5' oligonucleotides.

Abstract: The invention provides novel oligonucleotides that form a duplex with a target nucleic acid and then form a triplex with the duplex formed between the oligonucleotide and the target nucleic acid.