Patents by Inventor Eleanor A. Miele

Eleanor A. Miele has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Autocatalytic replication of recombinant RNA
    Patent number: 5620870
    Abstract: This invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis and a heterologous sequence of interest inserted at a specific site in the internal region of the recombinant molecule. Such recombinant RNA molecules are capable of serving as a template for the synthesis of complementary single-stranded molecules by RNA-directed RNA polymerase. The product molecules so formed are also capable of serving as a template for the synthesis of additional copies of the original recombinant RNA molecule. In a preferred embodiment of the invention Q.beta. replicase is used as the RNA-directed RNA polymerase.
    Type: Grant
    Filed: December 5, 1994
    Date of Patent: April 15, 1997
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Fred R. Kramer, Eleanor A. Miele, Donald R. Mills
  • Cell-free method for synthesizing a protein
    Patent number: 5602001
    Abstract: This invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis-and a heterologous sequence of interest inserted at a specific site in the internal region of the recombinant molecule. Such recombinant RNA molecules are capable of serving as a template for the synthesis of complementary single-stranded molecules by RNA-directed RNA polymerase. The product molecules so formed are also capable of serving as a template for the synthesis of additional copies of the original recombinant RNA molecule. In a preferred embodiment of the invention Q.beta. replicase is used as the RNA-directed RNA polymerase. The invention also concerns methods of selectively cleaving RNA molecules at specific sites, methods of constructing recombinant RNA molecules, and methods for the autocatalytic in vitro replication of recombinant RNA molecules.
    Type: Grant
    Filed: April 29, 1994
    Date of Patent: February 11, 1997
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Fred R. Kramer, Eleanor A. Miele, Donald R. Mills
  • Autocatalytic replication of recombinant RNA
    Patent number: 4786600
    Abstract: This invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis and a heterologous sequence of interest inserted at a specific site in the internal region of the recombinant molecule. Such recombinant RNA molecules are capable of serving as a template for the synthesis of complementary single-stranded molecules by RNA-directed RNA polymerase. The product molecules so formed are also capable of serving as a template for the synthesis of additional copies of the original recombinant RNA molecule. In a preferred embodiment of the invention Q.beta. replicase is used as the RNA-directed RNA polymerase.
    Type: Grant
    Filed: May 25, 1984
    Date of Patent: November 22, 1988
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Fred R. Kramer, Eleanor A. Miele, Donald R. Mills