Abstract: The present invention provides bio-nanoparticles (BioNPs) for delivering an active agent into hematopoietic stem & progenitor cells (HSPCs). Each BioNP comprises a core and a biological membrane covering the core, which comprises the active agent and a polymer. The biological membrane comprises a phospholipid bilayer and one or more surface proteins of a megakaryocyte (Mk). The active agent remains active after being delivered into the HSPC. Also provided are methods for preparing the BioNPs and uses of the BioNPs for targeted delivery of an active agent into HSPCs and/or treating or preventing a disease or condition in a subject in need thereof.

Abstract: A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.

Abstract: The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.

Abstract: The present invention provides a method for increasing production of a metabolite by a non-naturally occurring methylotroph, comprising growing the non-naturally occurring methylotroph in a medium comprising methanol. Expression of one or more native genes in the non-naturally occurring methylotroph is changed. Also provided are the non-naturally occurring methylotroph and preparation thereof.

Abstract: Syntrophic co-cultures containing at least two microorganisms, wherein (a) at least one of the microorganisms is a solventogen able to metabolize biomass to produce metabolic byproduct(s) therefrom, (b) at least one of the microorganisms is a microorganism different from the solventogen, wherein the microorganism different from the primary solventogen depends on the metabolites and/or the metabolism of the primary solventogen for survival and growth and is able to fix or metabolize the metabolic byproducts produced by the solventogen to produce metabolic byproduct(s) therefrom, and (c) the solventogen is able to metabolize the metabolic byproduct(s) produced by the microorganism different from the solventogen to produce further metabolic byproducts, such as liquid fuels and commodity chemicals, as well as methods for using these syntrophic co-cultures to produce such products via fermentation, are disclosed.

Abstract: A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.

Abstract: The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.

Abstract: A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.

Abstract: A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.

Abstract: The present invention relates to a recombinant E. coli exhibiting a complex phenotype, comprising one or more RNA polymerase subunit genes, one or more functional genes, and, optionally, one or more transcription factors from a heterologous prokaryote. Also provided are methods for screening such a recombinant E. coli.

Abstract: The present invention relates to methods and compositions for engineering sporulating bacterial cells, particularly a cell of the class Clostridia. In particular, the present invention relates to the generation of sporulation deficient bacteria for the generation of industrial superior phenotypes.

Abstract: The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.

Abstract: The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species.

Abstract: The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species.

Abstract: The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.

Abstract: The present invention relates to a recombinant E. coli exhibiting a complex phenotype, comprising one or more RNA polymerase subunit genes, one or more functional genes, and, optionally, one or more transcription factors from a heterologous prokaryote. Also provided are methods for screening such a recombinant E. coli.

Abstract: The present invention relates to a recombinant E. coli exhibiting a complex phenotype, comprising three or more heterologous DNA fragments integrated into a chromosome of the recombinant E. coli bacterium. Also provided are methods for screening such a recombinant E. coli.

Abstract: Expression of the SpoIIE gene in a solventogenic Clostridium cell is silenced and sporulation is abolished. The cell exhibits increased production of a solvent, such as butanol, relative to a wild-type solventogenic Clostridium cell and can be used for industrial-scale production of a chemical product. A method includes silencing the SpoIIE gene of the Clostridium cell via a homologous recombination method in which a resolvase gene is expressed. Another method includes increasing solvent production in bacterial cells by inoculating the cells with an inoculum of exponentially growing cells, wherein expression of a sporulation gene in the bacterial cells is inhibited or silenced and the cells of the inoculum are in a post-exponential phase of growth prior to inoculation.

Abstract: The present invention relates to metabolic engineering issues related to flux determinism in core primary-metabolism pathways. In particular, the present invention relates to alcohol (e.g., butanol) production and selectivity, and related methods thereof.

Abstract: The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species.