Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.

Abstract: Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.

Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.

Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.

Abstract: An online business method and system enables donors or parents or guardians of donors to order and purchase stem-cells extracted from biological tissue sampled from the donor, such as, for example, cord-blood stem cells of a newborn baby, wherein the ordering process interfaces directly with the attending medical services, and the service steps include collection, extraction, preservation, containment, packaging, delivery and storage of the stem cells in a storage medium that can be cost-effectively maintained in a storage facility or by the donor, parent or guardian at home. In one embodiment, preservation is by freeze-drying, containment is in a vacuum vial, and storage is at room temperature.

Abstract: The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, up-regulated or down-regulated.

Abstract: Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes

Abstract: An online business method and system enables donors or parents or guardians of donors to order and purchase stem-cells extracted from biological tissue sampled from the donor, such as, for example, cord-blood stem cells of a newborn baby, wherein the ordering process interfaces directly with the attending medical services, and the service steps include collection, extraction, preservation, containment, packaging, delivery and storage of the stem cells in a storage medium that can be cost-effectively maintained in a storage facility or by the donor, parent or guardian at home. In one embodiment, preservation is by freeze-drying, containment is in a vacuum vial, and storage is at room temperature.

Abstract: An online business method and system enables donors or parents or guardians of donors to order and purchase stem-cells from biological tissue sampled from the donor, such as, for example, cord-blood stem cells of a newborn baby, wherein the ordering process interfaces directly with the attending medical services, and the service steps include collection, extraction, preservation, containment, packaging, delivery and storage of the stem cells in a storage medium that can be cost-effectively maintained by the donor, parent or guardian at home or in a custodial location. In one embodiment, preservation is by freeze-drying, containment is in a vacuum vial, and storage is at room temperature.

Abstract: An online business method and system enables donors or parents or guardians of donors to order and purchase stem-cells from biological tissue sampled from the donor, such as, for example, cord-blood stem cells of a newborn baby, wherein the ordering process interfaces directly with the attending medical services, and the service steps include collection, extraction, preservation, containment, packaging, delivery and storage of the stem cells in a storage medium that can be cost-effectively maintained by the donor, parent or guardian at home or in a custodial location. In one embodiment, preservation is by freeze-drying, containment is in a vacuum vial, and storage is at room temperature.