Patents by Inventor Elena Vitalievna Klyachko

Elena Vitalievna Klyachko has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for producing lower alkyl ester
    Patent number: 9708637
    Abstract: A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3?-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: July 18, 2017
    Assignee: AJINOMOTO CO., INC.
    Inventors: Rustem Saidovich Shakulov, Elena Vitalievna Klyachko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh
  • Method for Producing Amino Acids Using Bacterium of the Enterobacteriaceae Family
    Publication number: 20160265019
    Abstract: A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3?-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.
    Type: Application
    Filed: May 25, 2016
    Publication date: September 15, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Rustem Saidovich Shakulov, Elena Vitalievna Klyachko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh
  • L-amino acid producing bacterium
    Patent number: 9376695
    Abstract: A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3?-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: June 28, 2016
    Assignee: AJINOMOTO CO., INC.
    Inventors: Rustem Saidovich Shakulov, Elena Vitalievna Klyachko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh
  • Method for Producing L-Amino Acids Using Bacteriua of the Enterobacteriaceae Family
    Publication number: 20140287472
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Application
    Filed: June 11, 2014
    Publication date: September 25, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for producing L-amino acids using bacteria of the enterobacteriaceae family
    Patent number: 8785161
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Grant
    Filed: May 8, 2012
    Date of Patent: July 22, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for producing L-amino acids using bacteria of the enterobacteriaceae family
    Patent number: 8728774
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Grant
    Filed: February 22, 2011
    Date of Patent: May 20, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • METHOD FOR PRODUCING L-AMINO ACIDS USING BACTERIA OF THE ENTEROBACTERIACEAE FAMILY
    Publication number: 20120219996
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Application
    Filed: May 8, 2012
    Publication date: August 30, 2012
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Mutant phosphoribosylpyrophosphate synthetase and method for producing L-histidine
    Patent number: 8071339
    Abstract: The present invention relates to a mutant bacterial PRPP synthetase which is resistant to feedback by purine nucleotides, and a method for producing L-histidine using the bacterium of the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by use of the PRPP synthetase which is resistant to feedback by purine nucleotides, coded by the mutant prsA gene.
    Type: Grant
    Filed: February 15, 2008
    Date of Patent: December 6, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Elena Vitalievna Klyachko, Rustem Saidovich Shakulov, Yuri Ivanovich Kozlov
  • Method for producing L-amino acids by fermentation using bacteria having enhanced expression of xylose utilization genes
    Patent number: 8003367
    Abstract: A method for producing an L-amino acid, such as L-histidine, L-threonine, L-lysine, L-glutamic acid, and L-tryptophan, using bacterium belonging to the genus Escherichia which has increased expression of genes, such as those of the xylABFGHR locus, which encode the xylose utilization enzymes, is disclosed. The method includes cultivating the L-amino acid producing bacterium in a culture medium containing xylose, and collecting the L-amino acid from the culture medium.
    Type: Grant
    Filed: March 15, 2005
    Date of Patent: August 23, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Aleksey Nikolaevich Marchenko, Sergey Vladimirovich Benevolensky, Elena Vitalievna Klyachko, Yuri Ivanovich Kozlov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Method for producing L-amino acids by fermentation using bacteria having enhanced expression of xylose utilization genes
    Patent number: 8003368
    Abstract: A method for producing an L-amino acid, such as L-histidine, L-threonine, L-lysine, L-glutamic acid, and L-tryptophan, using bacterium belonging to the genus Escherichia which has increased expression of genes, such as those of the xylABFGHR locus, which encode the xylose utilization enzymes, is disclosed. The method includes cultivating the L-amino acid producing bacterium in a culture medium containing xylose, and collecting the L-amino acid from the culture medium.
    Type: Grant
    Filed: January 7, 2009
    Date of Patent: August 23, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Aleksey Nikolaevich Marchenko, Sergey Vladimirovich Benevolensky, Elena Vitalievna Klyachko, Yuri Ivanovich Kozlov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • METHOD FOR PRODUCING L-AMINO ACIDS USING BACTERIA OF THE ENTEROBACTERIACEAE FAMILY
    Publication number: 20110143403
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Application
    Filed: February 22, 2011
    Publication date: June 16, 2011
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for producing L-amino acids using bacteria of the Enterobacteriaceae family
    Patent number: 7915018
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Grant
    Filed: October 12, 2005
    Date of Patent: March 29, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for producing L-histidine using bacteria which has an enhanced gene produced
    Patent number: 7785860
    Abstract: A method is provided for producing L-histidine using bacterium of the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of the AICAR transformylase-IMP cyclohydrolase encoded by the purH gene.
    Type: Grant
    Filed: August 27, 2004
    Date of Patent: August 31, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Elena Vitalievna Klyachko, Rustem Saidovich Shakulov, Yuri Ivanovich Kozlov
  • Method for producing a non-aromatic L-amino acid using a bacterium of the family having expression of the csrA gene attenuated
    Patent number: 7771976
    Abstract: The present invention provides a method for producing a non-aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the csrA gene.
    Type: Grant
    Filed: July 31, 2007
    Date of Patent: August 10, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Andrey Yurievich Gulevich, Danila Vadimovich Zimenkov, Elena Vitalievna Klyachko, Tatyana Viktorovna Leonova, Yury Ivanovich Koslov, Vitaly Grigorievich Paraskevov, legal representative
  • Mutant Phosphoribosylpyrophosphate Synthetase and Method for Producing L-Histidine
    Publication number: 20090275089
    Abstract: The present invention relates to a mutant bacterial PRPP synthetase which is resistant to feedback by purine nucleotides, and a method for producing L-histidine using the bacterium of the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by use of the PRPP synthetase which is resistant to feedback by purine nucleotides, coded by the mutant prsA gene.
    Type: Application
    Filed: February 15, 2008
    Publication date: November 5, 2009
    Inventors: Elena Vitalievna Klyachko, Rustem Saidovich Shakulov, Yuri Ivanovich Kozlov
  • Method for Producing a Non-Aromatic L-Amino Acid Using a Bacterium of the Enterobacteriaceae Family Having Expression of the csrA Gene Attenuated
    Publication number: 20090239266
    Abstract: The present invention provides a method for producing a non-aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the csrA gene.
    Type: Application
    Filed: July 31, 2007
    Publication date: September 24, 2009
    Inventors: Andrey Yurievich Gulevich, Danila Vadimovich ZIMENKOV, Elena Vitalievna KLYACHKO, Tatyana Viktorovna LEONOVA, Yury Ivanovich Kozlov, Vitaly Grigorievich PARASKEVOV
  • METHOD FOR PRODUCING AMINO ACIDS USING BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY
    Publication number: 20090137010
    Abstract: A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3?-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.
    Type: Application
    Filed: September 26, 2008
    Publication date: May 28, 2009
    Inventors: Rustem Saidovich Shakulov, Elena Vitalievna Klyachko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh
  • METHOD FOR PRODUCING L-AMINO ACIDS BY FERMENTATION USING BACTERIA HAVING ENHANCED EXPRESSION OF XYLOSE UTILIZATION GENES
    Publication number: 20090117623
    Abstract: A method for producing an L-amino acid, such as L-histidine, L-threonine, L-lysine, L-glutamic acid, and L-tryptophan, using bacterium belonging to the genus Escherichia which has increased expression of genes, such as those of the xylABFGHR locus, which encode the xylose utilization enzymes, is disclosed. The method includes cultivating the L-amino acid producing bacterium in a culture medium containing xylose, and collecting the L-amino acid from the culture medium.
    Type: Application
    Filed: January 7, 2009
    Publication date: May 7, 2009
    Inventors: Aleksey Nikolaevich Marchenko, Sergey Vladimirovich Benevolensky, Elena Vitalievna Klyachko, Yuri Ivanovich Kozlov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Method for producing L-histidine using bacteria of Enterobacteriaceae family
    Patent number: 7399618
    Abstract: A method is provided for producing L-histidine using bacterium of the Enterobacteriaceae family, wherein the L-amino acid productivity of the bacterium is enhanced by enhancing an activity of the transaldolase encoded by the talB gene.
    Type: Grant
    Filed: April 4, 2007
    Date of Patent: July 15, 2008
    Assignee: Ajinomoto Co., Inc.
    Inventors: Elena Vitalievna Klyachko, Rustam Saidovich Shakulov, Yuri Ivanovich Kozlov
  • Bacteria of the family having enhanced transaldolase activity
    Patent number: 7300786
    Abstract: A method is provided for producing L-histidine using bacterium of the Enterobacteriaceae family, wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of the transaldolase encoded by the talb gene.
    Type: Grant
    Filed: July 16, 2004
    Date of Patent: November 27, 2007
    Assignee: Ajinomoto Co., Inc.
    Inventors: Elena Vitalievna Klyachko, Rustam Saidovich Shakulov, Yuri Ivanovich Kozlov