Patents by Inventor Eli A. Hefner

Eli A. Hefner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200270677
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Application
    Filed: March 30, 2020
    Publication date: August 27, 2020
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: John F. REGAN, Dianna MAAR, Dawne N. SHELTON, Tina C. LEGLER, Samantha COOPER, Wei YANG, Eli A. HEFNER, Niels KLITGORD
  • Publication number: 20200209229
    Abstract: Described are methods and compositions for sequential multiplex detection of target analytes in a sample. The method comprises contacting the sample comprising analytes immobilized on a solid support with binding agents that specifically bind an analyte in the sample, wherein each of the binding agents binds a different analyte and is attached to a single-stranded nucleic acid molecule comprising a unique sequence. The sample is then contacted with a labeled complementary nucleic acid molecule that binds the single-stranded nucleic acid molecule attached to one binding agent. The signal from the label is detected, and then reduced or eliminated. The sample can be simultaneously contacted with a second labeled complementary nucleic acid molecule that binds a different binding agent, and the signal from the second label is detected. The process is repeated for each additional analyte in the sample, thereby sequentially detecting the presence of the analytes in the sample.
    Type: Application
    Filed: December 20, 2019
    Publication date: July 2, 2020
    Inventors: William STRONG, Eli HEFNER
  • Patent number: 10604789
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: March 31, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Publication number: 20190284610
    Abstract: Compositions, kits, and methods are provided for the normalization of a quantitative polymerase chain reaction (PCR) amplification. Also provided are compositions, kits, and methods for multiplexing qPCR amplification of two or more target nucleic acids in the same well.
    Type: Application
    Filed: March 14, 2019
    Publication date: September 19, 2019
    Inventors: Eli HEFNER, Yann JOUVENOT
  • Publication number: 20190119736
    Abstract: Methods of, and compositions for, analysis using digital amplification assays with unconventional/inverse changes in photoluminescence to indicate the presence of one or more targets. In an exemplary method, isolated volumes may be formed, only a subset of which contain a target. Each volume may include a probe having a label, a sink having a quencher and configured to hybridize with the probe to quench the label, and a separator configured to hybridize with the probe and/or the sink to block hybridization of the sink with the probe. An amplification reaction may be performed in the volumes to generate an amplicon corresponding to the target. The separator may hybridize with the amplicon, and may be extended or degraded by the amplification reaction. Photoluminescence of the label may be detected from the volumes, and the photoluminescence of target-positive volumes may be less than that of target-negative volumes.
    Type: Application
    Filed: October 12, 2018
    Publication date: April 25, 2019
    Inventors: Eli A. HEFNER, Dianna MAAR
  • Patent number: 10066256
    Abstract: Methods, compositions, and kits are provided for quantifying a number or frequency of double stranded breaks in the genome of a cell or in the genomes of a population of cells.
    Type: Grant
    Filed: March 17, 2016
    Date of Patent: September 4, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yann Jouvenot, Nick Heredia, Viresh Patel, Samantha Cooper, Jen Berman, Eli Hefner
  • Patent number: 9850517
    Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 ?m or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: December 26, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Tom McCollum, Paul Patt, Frank Shen, Daniel Y. Chu, Don Flory, Mike Griffin, Xin Heng, Eli Hefner
  • Publication number: 20170166957
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Application
    Filed: January 20, 2017
    Publication date: June 15, 2017
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Patent number: 9556475
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Grant
    Filed: August 12, 2014
    Date of Patent: January 31, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Publication number: 20160273023
    Abstract: Methods, compositions, and kits are provided for quantifying a number or frequency of double stranded breaks in the genome of a cell or in the genomes of a population of cells.
    Type: Application
    Filed: March 17, 2016
    Publication date: September 22, 2016
    Inventors: Yann Jouvenot, Nick Heredia, Viresh Patel, Samantha Cooper, Jen Berman, Eli Hefner
  • Patent number: 9354155
    Abstract: An automated system captures multiple digital images of a sample, from different viewing areas of the sample. The images are analyzed and a test result may be reported based on the count of cells in all of the digital images. The system may be especially applicable to the testing of samples having low cell concentrations, as the variability between tests may be reduced as compared with a system that bases its test results on only a single image of the sample. The system may count the cells in a first digital image of the sample, and may capture or analyze one or more additional digital images only when it is recognized that additional measurements are needed to ensure adequate accuracy and repeatability of the test.
    Type: Grant
    Filed: May 30, 2012
    Date of Patent: May 31, 2016
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Daniel Chu, Eli A. Hefner, Yann Jouvenot
  • Publication number: 20150148250
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Application
    Filed: August 12, 2014
    Publication date: May 28, 2015
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Publication number: 20140162266
    Abstract: This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference). In some cases, the method involves combining a query nucleic acid sample with a diluting nucleic acid sample and measuring the relative copy number of a target sequence compared with a reference sequence in the combined sample.
    Type: Application
    Filed: December 5, 2013
    Publication date: June 12, 2014
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Niels Klitgord, John Regan, Svilen Tzonev, Eli Hefner, Jennifer R. Berman
  • Publication number: 20140024107
    Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000000 pixels each having an area of 2×2 ?m or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.
    Type: Application
    Filed: September 27, 2013
    Publication date: January 23, 2014
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Tom McCOLLUM, Paul PATT, Frank SHEN, Daniel Y. CHU, Don FLORY, Mike GRIFFIN, Xin HENG, Eli HEFNER
  • Patent number: 8570370
    Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 ?m or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.
    Type: Grant
    Filed: August 27, 2010
    Date of Patent: October 29, 2013
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Tom McCollum, Paul Patt, Frank Shen, Daniel Y. Chu, Don Flory, Mike Griffin, Xin Heng, Eli Hefner
  • Publication number: 20120314092
    Abstract: An automated system captures multiple digital images of a sample, from different viewing areas of the sample. The images are analyzed and a test result may be reported based on the count of cells in all of the digital images. The system may be especially applicable to the testing of samples having low cell concentrations, as the variability between tests may be reduced as compared with a system that bases its test results on only a single image of the sample. The system may count the cells in a first digital image of the sample, and may capture or analyze one or more additional digital images only when it is recognized that additional measurements are needed to ensure adequate accuracy and repeatability of the test.
    Type: Application
    Filed: May 30, 2012
    Publication date: December 13, 2012
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Daniel Chu, Eli A. Hefner, Yann Jouvenot
  • Publication number: 20110211058
    Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 ?m or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.
    Type: Application
    Filed: August 27, 2010
    Publication date: September 1, 2011
    Applicant: BIO-RAD LABORATORIES, INC.
    Inventors: Tom McCollum, Paul Patt, Frank Shen, Daniel Y. Chu, Don Flory, Mike Griffin, Xin Heng, Eli Hefner