Abstract: Compositions and methods for treating aggregation-associated diseases are disclosed. In particular, compositions comprising the nascent polypeptide-associated complex (NAC) and the apical domain of CCT1 as well as peptide fragments thereof and fusion proteins containing NAC and CCT1 peptides can be used to suppress pathological protein aggregation and are useful for treatment of diseases associated with polyQ aggregation, amyloid beta aggregation, and alpha-synuclein aggregation.

Abstract: The invention relates to a method and a prokaryotic cell for identifying a compound, which interferes with ribosome biogenesis, assembly and/or degradation. The cell expresses a first fusion protein comprising a first ribosomal protein, an amino acid linker and a first fluorescent protein, and a second fusion protein comprising a second ribosomal protein, an amino acid linker and a second fluorescent protein. The invention further relates to a gene construct comprising a first element encoding a first ribosomal protein fused to a first fluorescent protein by an amino acid linker, and a second element encoding a second ribosomal protein fused to a second fluorescent protein by an amino acid linker.

Abstract: The invention relates to a method and a prokaryotic cell for identifying a compound, which interferes with ribosome biogenesis, assembly and/or degradation. The cell expresses a first fusion protein comprising a first ribosomal protein, an amino acid linker and a first fluorescent protein, and a second fusion protein comprising a second ribosomal protein, an amino acid linker and a second fluorescent protein. The invention further relates to a gene construct comprising a first element encoding a first ribosomal protein fused to a first fluorescent protein by an amino acid linker, and a second element encoding a second ribosomal protein fused to a second fluorescent protein by an amino acid linker.

Abstract: There are provided methods for the expression of a recombinant protein of interest, said methods comprising, in additional to various additional steps: a) culturing a host cell which expresses: i) one or more genes encoding the recombinant protein(s) of interest; ii) at least two genes encoding proteins selected from the group consisting of the chaperone proteins GroEL, GRoES, Dnak, DnaJ, GRpe, ClpB and their homologs (for example, Hsp104, Ydj1 and Ssa1 in yeast); under conditions suitable for protein expression; and separating said recombinant protein of interest from the host cell culture. Also provided are methods for increasing the degree of refolding of a recombinant protein of interest by ading a composition containing a chaperone protein to a preparation of the recombinant protein of interest in vitro.