Abstract: Methods and systems for evaluating biological dataset profiles relating to inflammatory cardiovascular conditions are provided, where datasets comprising information for multiple cellular parameters are compared and identified, and used in the evaluation of candidate pharmacologic agents for suitability as therapeutic agents.

Abstract: Methods and systems for evaluating biological dataset profiles relating to inflammatory cardiovascular conditions are provided, where datasets comprising information for multiple cellular parameters are compared and identified, and used in the evaluation of candidate pharmacologic agents for suitability as therapeutic agents.

Abstract: A method of screening biologically active agent based on the analysis of complex biological responses in culture. Methods for selecting cells and culture conditions for such screens are provided, as well as the identification of an optimized set of discrete parameters to be measured, and the use of biomap analysis for rapid identification and characterization of drug candidates, genetic sequences acting pathways, and the like. A feature of the invention is simultaneous screening of a large number of cellular pathways, and the rapid identification of compounds that cause cellular responses.

Abstract: A method of screening biologically active agent based on the analysis of complex biological responses in culture. Methods for selecting cells and culture conditions for such screens are provided, as well as the identification of an optimized set of discrete parameters to be measured, and the use of biomap analysis for rapid identification and characterization of drug candidates, genetic sequences acting pathways, and the like. A feature of the invention is simultaneous screening of a large number of cellular pathways, and the rapid identification of compounds that cause cellular responses.

Abstract: The involvement of an expression product in a cell in a pathway is determined by genetically modifying the cell, incubating the cell with predetermined factors in induce a physiological state and measuring parameters affected by the pathway. Changes in the levels of the parameters as a result of the presence of the expressed product indicate that the expression product is involved with the pathway.

Abstract: Methods and systems for evaluating biological dataset profiles relating to asthma and other atopic conditions are provided, where datasets comprising information for multiple cellular parameters are compared and identified, and used in the evaluation of candidate pharmacologic agents for suitability as therapeutic agents.

Abstract: The genes in a signaling pathway can be identified by over or under-expressing each member of a set of genes to be grouped in signaling pathways and measuring parameters under stimulatory conditions. Genes in a common signaling pathway give similar parameter profiles under such conditions. Moreover, the interactions between signaling pathways and the genes and gene products that mediate those interactions can be determined by measuring the response of those pathways to stimuli under conditions that reflect a complex cellular environment. The order in which genes and their products transmit signals through signaling pathways can be determined by assaying the effects of activators and inhibitors on those genes and gene products, and the mechanism of action of a compound of interest can be determined by comparing parameter profiles generated by the compound of interest to those generated with compounds of known mechanism of action.

Abstract: Compositions and methods are provided for the classification of biologically active agents according to their effect on human biology, through the use of complex, primary human cell-based disease models in scalable assay formats. The systems of the invention utilize the simultaneous activation of multiple signaling pathways to generate and identify patterns of expression of physiologically important cell surface and secreted molecules. Combinations of multiple cell types may be utilized. Systems encompassing multiple cell types not only respond to perturbations of each cell type's intracellular signaling networks, but also to inhibition of pathways of communication between cells. Readout information may be combined in multi-system analysis, where the profiles obtained from multiple systems are combined in order to provide enhanced resolution for agent classification.