Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

Abstract: Provided herein are compositions for gene modification or editing and methods of using same to treat or prevent certain conditions. Specific compositions and methods capable of safely and effectively editing gene targets expressed in the liver to durably lower LDL-C thereby treating a leading cause of cardiovascular disease are disclosed.

Abstract: Disclosed herein are novel gene editing systems capable of being delivered to a subject intravenously through a lipid nano particle pharmaceutical formulation and producing durable in vivo editing of a target gene, such as ANGPTL3, with high on-target gene editing efficiency, reduced or low off-target effect, and no germline editing. The gene editing systems comprise a chemically modified guide nucleic acid sequence with a spacer having a specified arrangement of deoxyribonucleotides and ribonucleotides. The novel gene editing systems comprise mRNA that encodes the gene editor proteins, which may include a modified nickase component. Methods of disease treatments using the gene editing systems are also disclosed.

Abstract: Provided herein are compositions for gene modification or editing and methods of using same to treat or prevent certain conditions. Specific compositions and methods capable of safely and effectively editing gene targets expressed in the liver to durably lower LDL-C thereby treating a leading cause of cardiovascular disease are disclosed.

Abstract: Provided herein are compositions, methods of making the same, and methods for targeted delivery of therapeutic agents for modifying expression and function of target genes, e.g. proteins involved in lipid and cholesterol metabolism such as PCSK9.

Abstract: Disclosed herein are PEG-DPCA conjugates having multiple hydrophobic DPCA groups at one or both terminal ends of a PEG compound and compositions thereof and methods of using thereof for tissue regeneration and/or cellular repair (e.g., wound healing).

Abstract: A method of forming an etched part includes forming a substrate including a thermoset resin and etching a surface of the substrate. The thermoset resin includes a vat photopolymerization (VPP) thermoset resin and at least one of an etchable phase and etchable particles disposed within the VPP thermoset resin. The etching removes the etchable phase from the VPP thermoset resin at the surface of the substrate such that a plurality of micro-mechanical bonding sites are formed on an etched surface of the substrate.

Abstract: In some aspects, the techniques described herein relate to a passenger motor vehicle including a robotic arm configured for use in performing an additive manufacturing operation. A method is also disclosed.

Abstract: A method of additive manufacturing a part via vat photopolymerization (VPP) includes irradiating a first material through a first transparent wall of a first material tank and forming a first section of an nth layer of the part on a platform, positioning the platform with the first section of the nth layer in a second material tank with a distance between the first section of the nth layer and a second transparent wall, and irradiating a second material such that a second section of the nth layer of the part is formed on the platform adjacent to the first section of the nth layer. The method repeats forming first and second sections of additional layer until a predetermined number of total layers are formed. Positioning the first and second sections with a distance between the second and first transparent walls, respectively, inhibits crashing during forming of the part.

Abstract: A method of forming an etched part includes forming a substrate including a thermoset resin and etching a surface of the substrate. The thermoset resin includes a vat photopolymerization (VPP) thermoset resin and at least one of an etchable phase and etchable particles disposed within the VPP thermoset resin. The etching removes the etchable phase from the VPP thermoset resin at the surface of the substrate such that a plurality of micro-mechanical bonding sites are formed on an etched surface of the substrate.

Abstract: A thermoset resin for forming parts to be metal plated includes a vat photopolymerization (VPP) thermoset resin and an etchable phase disposed in the VPP thermoset resin. The etchable phase is etched from a surface of a part formed from the VPP thermoset resin such that a plurality of micro-mechanical locking sites is formed on the surface of the part. The etchable phase is at least one of organic particles, organic resins, inorganic particles, and copolymers of the VPP thermoset resin. For example, the etchable phase can be a polybutadiene phase and/or a mineral such as calcium carbonate.

Abstract: An additive article stabilizing method includes prior to polymerization, adding a radiation-activated stabilizing composition to a liquid resin, forming the article from the liquid resin, layer by layer, using radiation such that the stabilizing composition does not stabilize the liquid resin but the formed article, and neutralizing free radicals generated during a degradation process initiated by exposure of the article to additional radiation post-cure.

Abstract: The invention encompasses devices and methods that at least partially entrap the droplets and aerosols from a person undergoing an aerosol/droplet-generating procedure, thereby reducing a heath care worker's direct exposure to such health hazards. The device includes a planar cover structure impermeable to aerosols, droplets, fluids and gas. The planar cover structure providing a barrier between a health care worker and the body cavity or orifice of a patient. A hollow tubular extension forming an opening on the planar cover structure and the opening is configured to he positioned approximately on top of the body cavity or orifice of the patient. The opening configured to be reversibly closed via a closure mechanism.

Abstract: A method of additive manufacturing a part via vat photopolymerization (VPP) includes irradiating a first material through a first transparent wall of a first material tank and forming a first section of an nth layer of the part on a platform, positioning the platform with the first section of the nth layer in a second material tank with a distance between the first section of the nth layer and a second transparent wall, and irradiating a second material such that a second section of the nth layer of the part is formed on the platform adjacent to the first section of the nth layer. The method repeats forming first and second sections of additional layer until a predetermined number of total layers are formed. Positioning the first and second sections with a distance between the second and first transparent walls, respectively, inhibits crashing during forming of the part.

Abstract: A thermoset resin for forming parts to be metal plated includes a vat photopolymerization (VPP) thermoset resin and an etchable phase disposed in the VPP thermoset resin. The etchable phase is etched from a surface of a part formed from the VPP thermoset resin such that a plurality of micro-mechanical locking sites is formed on the surface of the part. The etchable phase is at least one of organic particles, organic resins, inorganic particles, and copolymers of the VPP thermoset resin. For example, the etchable phase can be a polybutadiene phase and/or a mineral such as calcium carbonate.

Abstract: A method of forming a part includes 3D printing a photopolymerizable resin and forming a preformed part and subsequently post-curing the preformed part with electron beams. The preformed part may be cured via UV curing. A section of the preformed part post-cured with electron beams may have a thickness of at least 1.0 centimeter, for example, at least 2.0 centimeters or at least 3.0 centimeters. An electron beam dosage to post-cure the preformed part may be between 10 kilogray (kGy) and 100 kGy. The preformed part may be 3D printed using stereolithography (SLA), digital light processing (DLP) or material jetting (MJ) and the photopolymerizable resin may include at least one of an acrylate functional polymer and a methacrylate functional polymer. In the alternative, or in addition to, the photopolymerizable resin may include at least one of a urethane, a polyester, and a polyether.

Abstract: An additive article stabilizing method includes prior to polymerization, adding a radiation-activated stabilizing composition to a liquid resin, forming the article from the liquid resin, layer by layer, using radiation such that the stabilizing composition does not stabilize the liquid resin but the formed article, and neutralizing free radicals generated during a degradation process initiated by exposure of the article to additional radiation post-cure.

Abstract: An engine component includes stratified layers that form an integral cylinder head and an internally cooled valve guide located in a cylinder head guide boss. The integral cylinder head further includes a coolant passage within a body of the valve guide that is in fluid communication with a cylinder head cooling jacket.

Abstract: An additive manufacturing method. The method includes mixing a reflective material including fibers with a photopolymer resin to form a resin mixture having a loading of the reflective material of at least 3 wt. %. The method further includes curing the resin mixture using a light source to form first and second individually cured layers and a bridge of a portion of the fibers between the two layers.

Abstract: An additive manufacturing method. The method includes mixing a reflective material including fibers with a photopolymer resin to form a resin mixture having a loading of the reflective material of at least 3 wt. %. The method further includes curing the resin mixture using a light source to form first and second individually cured layers and a bridge of a portion of the fibers between the two layers.